This Toolkit collates a range of impact-related materials, including training documents and materials, worked examples, factsheets, lists of indicators, key publications, case studies, and presentation slides. These have been created and/or brought together using a bottom-up approach that tries to reflect the varied needs of Nodes in ELIXIR member countries. The primary audiences of this Toolkit are those in ELIXIR (e.g. operators, developers and managers of bioinformatics resources) wishing to demonstrate and communicate the performance, impact, and ultimately public value of their research infrastructure, to their particular set of funders and stakeholders. Sibling research infrastructures in the life sciences and beyond may also find the Toolkit useful.