VIB Bioinformatics Core

Provider of Bioinformatics and software training, plus informatics services and resource management support.

VIB Bioinformatics Core https://tess.elixir-europe.org/content_providers/bitsvib Provider of Bioinformatics and software training, plus informatics services and resource management support. /system/content_providers/images/000/000/042/original/bioinformatics_core_cmyk_pos.jpg?1539150443
Showing 48 materials out of 48. View all results.
A tour of machine learning - classification

Machine learning has become ubiquitous in biotechnology (as in many other fields), fueled largely by the increasing availability and amount of data. Learning algorithms can figure out how to perform important tasks by generalizing examples. Typical applications are diagnoses/prognoses,...

Scientific topics: Machine learning

A tour of machine learning - classification https://tess.elixir-europe.org/materials/a-tour-of-machine-learning-classification Machine learning has become ubiquitous in biotechnology (as in many other fields), fueled largely by the increasing availability and amount of data. Learning algorithms can figure out how to perform important tasks by generalizing examples. Typical applications are diagnoses/prognoses, gene/protein annotation, drug design, image recognition, text mining and many others. However, building successful machine learning models requires a substantial amount of “black art” that is hard to find in textbooks. This course is an interactive Jupyter Notebook (Python) that will teach you how to build successful machine learning models. No background in machine learning is assumed, just a keen interest. Machine learning Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22
RNA-Seq analysis for differential expression

We first perform quality control of the sequence reads to detect biases or leftover adaptors. We then map the reads to the reference genome with use of a transcript database model. We perform a detailed QC analysis of the mapping results to again detect potential problems. The mapping data is...

Scientific topics: RNA-Seq, Gene expression

RNA-Seq analysis for differential expression https://tess.elixir-europe.org/materials/rna-seq-analysis-for-differential-expression We first perform quality control of the sequence reads to detect biases or leftover adaptors. We then map the reads to the reference genome with use of a transcript database model. We perform a detailed QC analysis of the mapping results to again detect potential problems. The mapping data is adjusted to compensate for artifacts like duplicates. The mappings are used to obtain transcript counts usable for differential expression. The count tables are merged and used to compute differential expression using several programs. We START a typical functional analysis of the obtained results similar to what is done for microarray data in order to exemplify handy tools and commercial alternatives. The code pieces used during the training are made available through our Wiki as well as detailed results and can be copied and adapted for own user needs with minimal edits. Key results have been saved to our server and can be downloaded to fully reproduce the training. RNA-Seq Gene expression Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22 2017-10-09
Downstream processing of RNA-Seq data in GenePattern

- Run RNA-seq downstream processing analyses in GenePattern, such as clustering, classification, GSEA... - Use GenePattern to save workflows, ensure reproducibility and share methodologies with colleagues and reviewers - Use the GenePattern Notebook environment to create documents that interleave...

Scientific topics: RNA-Seq

Downstream processing of RNA-Seq data in GenePattern https://tess.elixir-europe.org/materials/downstream-processing-of-rna-seq-data-in-genepattern - Run RNA-seq downstream processing analyses in GenePattern, such as clustering, classification, GSEA... - Use GenePattern to save workflows, ensure reproducibility and share methodologies with colleagues and reviewers - Use the GenePattern Notebook environment to create documents that interleave formatted text, graphics and other multimedia, executable code, and GenePattern analyses, creating a single "research narrative" that puts scientific discussion and analyses in the same place Summary The workshop will cover the use of GenePattern for downstream processing of high-throughput mRNA sequencing (RNA-Seq) data produced by Illumina sequencing machines.  This workshop is intended for non-programming biologists. 1. Running Analyses in GenePattern 2. Clustering 3. Prediction and Clasification 4. Working with analysis workflows: GenePattern pipelines 5. Working with Samples and Features 6. The GenePattern Notebook environment 7. Related tools: IGV, GSEA 8. Open Q&A The data files, slides and exercises will be made available after the workshop.   RNA-Seq Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Cloning with SnapGene

You will acquire the basic techniques that are used in recombinant DNA technology, particularly restriction enzymes, modification enzymes, polymerase chain reaction (PCR), plasmids (different vectors, mechanism to select and detect), site directed mutagenesis, heterologous gene expression in...

Scientific topics: Gene expression analysis

Cloning with SnapGene https://tess.elixir-europe.org/materials/cloning-with-snapgene You will acquire the basic techniques that are used in recombinant DNA technology, particularly restriction enzymes, modification enzymes, polymerase chain reaction (PCR), plasmids (different vectors, mechanism to select and detect), site directed mutagenesis, heterologous gene expression in prokaryotes/eukaryotes. You will be able to use a cloning program like CLC Bio Main Workbench or SnapGene. Gene expression analysis Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Hands-on introduction to ChIP-Seq analysis

This training gives an introduction to ChIP-seq data analysis, covering the processing steps starting from the reads to the peaks. Among all possible downstream analyses, the practical aspect will focus on motif analyses. A particular emphasis will be put on deciding which downstream analyses to...

Scientific topics: Mapping, ChIP-seq

Hands-on introduction to ChIP-Seq analysis https://tess.elixir-europe.org/materials/hands-on-introduction-to-chip-seq-analysis This training gives an introduction to ChIP-seq data analysis, covering the processing steps starting from the reads to the peaks. Among all possible downstream analyses, the practical aspect will focus on motif analyses. A particular emphasis will be put on deciding which downstream analyses to perform depending on the biological question. This training does not cover all methods available today. It does not aim at bringing users to a professional NGS analyst level but provides enough information to allow biologists understand what DNA sequencing practically is and to communicate with NGS experts for more in-depth needs. Mapping ChIP-seq Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22 2017-10-09
Comparative genomics in eukaryotes

This course presents a comprehensive overview of the principles and applications of comparative genomics in eukaryotes. The course combines lectures with hands-on exercises.

Comparative genomics in eukaryotes https://tess.elixir-europe.org/materials/comparative-genomics-in-eukaryotes This course presents a comprehensive overview of the principles and applications of comparative genomics in eukaryotes. The course combines lectures with hands-on exercises. Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Analysis of public microarray data sets

This training will give you an overview of what GEO has to offer. Several experiments will be analyzed using simple tools to obtain differential gene lists. An introduction to downstream tools dedicated to functional enrichment will close the session.

Scientific topics: Expression data visualisation

Analysis of public microarray data sets https://tess.elixir-europe.org/materials/analysis-of-public-microarray-data-sets This training will give you an overview of what GEO has to offer. Several experiments will be analyzed using simple tools to obtain differential gene lists. An introduction to downstream tools dedicated to functional enrichment will close the session. Expression data visualisation Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Analyzing biological networks with Ingenuity Pathway Analysis

IPA is software that allows researchers to analyze, integrate and understand data derived from experiments that generate a list of relevant genes, proteins or chemicals. The software relies on the Ingenuity Knowledge Base, a thoroughly curated database of high quality information on biological...

Analyzing biological networks with Ingenuity Pathway Analysis https://tess.elixir-europe.org/materials/analyzing-biological-networks-with-ingenuity-pathway-analysis IPA is software that allows researchers to analyze, integrate and understand data derived from experiments that generate a list of relevant genes, proteins or chemicals. The software relies on the Ingenuity Knowledge Base, a thoroughly curated database of high quality information on biological interactions and functional annotation. The database is limited to information on human, mouse and rat. Life Science Researchers PhD students post-docs 2016-04-22 2016-06-15
Building bioinformatics workflows

This course presents a comprehensive overview of the principles and applications of scientific workflow systems. The course combines lectures with hands-on exercises in which the participants learn to apply these principles to real biomedical datasets. The course will be based on freely available...

Building bioinformatics workflows https://tess.elixir-europe.org/materials/building-bioinformatics-workflows This course presents a comprehensive overview of the principles and applications of scientific workflow systems. The course combines lectures with hands-on exercises in which the participants learn to apply these principles to real biomedical datasets. The course will be based on freely available open source software and more specifically on Galaxy and KNIME. Life Science Researchers PhD students post-docs beginner bioinformaticians 2016-04-22 2016-06-15
Data Carpentry Workshop - Data Management beyond Excel

The main ambition of this workshop is that researchers will be able to handle their data in a more reproducible and efficient way. They should be able to retrieve, view, manipulate, process and manage their own and other one's data. The following topics will be covered during the workshop: ...

Data Carpentry Workshop - Data Management beyond Excel https://tess.elixir-europe.org/materials/data-carpentry-workshop-data-management-beyond-excel The main ambition of this workshop is that researchers will be able to handle their data in a more reproducible and efficient way. They should be able to retrieve, view, manipulate, process and manage their own and other one's data. The following topics will be covered during the workshop: How to use spreadsheet programs (e.g. Excel) more efficiently: what you can and can't do Getting data out of spreadsheets and into more powerful tools (e.g. R) Using databases, managing and querying data in SQL Workflows and automating repetitive tasks, including using command line PhD students post-docs Life Science Researchers 2016-04-22 2016-09-13
Data manipulation in OpenRefine

Most statistical and data mining tools assume that the data files are in a specific format, often completely different from the format that is created by the instrument that generates the data. Moreover, most of these tools only provide limited functionality for getting the raw data files in the...

Data manipulation in OpenRefine https://tess.elixir-europe.org/materials/data-manipulation-in-openrefine Most statistical and data mining tools assume that the data files are in a specific format, often completely different from the format that is created by the instrument that generates the data. Moreover, most of these tools only provide limited functionality for getting the raw data files in the correct format. OpenRefine is a powerful tool for cleaning up messy data and transforming it in the required format.  Life Science Researchers PhD students post-docs 2016-04-22 2016-06-15
Introductory session on programming with Bioperl

On the first day, we will refresh Perl syntax and types of variables. This basic knowledge will serve as a starting point to explain the use of subroutines, and how to make a module of your subroutine. On the second day we will prepare for Bioperl by explaining how you can reuse Perl code....

Introductory session on programming with Bioperl https://tess.elixir-europe.org/materials/introductory-session-on-programming-with-bioperl On the first day, we will refresh Perl syntax and types of variables. This basic knowledge will serve as a starting point to explain the use of subroutines, and how to make a module of your subroutine. On the second day we will prepare for Bioperl by explaining how you can reuse Perl code. Object-oriented programming will only be explained to the extent necessary for using Bioperl. We will write our first simple program in Bioperl and explore places where you can find information on the Bioperl modules. On the third day we will practice Bioperl further in real-life examples like sequence processing and applications that are defined by the participants. By the end of the session, you will understand Bioperl, and have already some first programming skills in Bioperl, which will allow you to practice further at an accelerated speed in order to becoming proficient in Bioperl. Life Science Researchers PhD students post-docs beginner bioinformaticians 2016-04-22 2016-06-15
Introduction to network biology

This course presents a comprehensive overview of the principles and applications of studying molecular networks. The course combines lectures with hands-on exercises in which the participants learn to apply these principles to real data. The course will be based on freely available data and...

Introduction to network biology https://tess.elixir-europe.org/materials/introduction-to-network-biology This course presents a comprehensive overview of the principles and applications of studying molecular networks. The course combines lectures with hands-on exercises in which the participants learn to apply these principles to real data. The course will be based on freely available data and software. In particular, special attention will go to the use of Cytoscape and its extensions. PhD students Life Science Researchers post-docs 2016-04-22 2017-04-14
Text mining and curation

This training will explain the basics of text mining, presenting the opportunities as well as the challenges of automatically extracting information from the literature. Further, the course will demonstrate how text mining data can be incorporated into a variety of applications and research...

Scientific topics: Data submission, annotation and curation, Natural language processing

Text mining and curation https://tess.elixir-europe.org/materials/text-mining-and-curation This training will explain the basics of text mining, presenting the opportunities as well as the challenges of automatically extracting information from the literature. Further, the course will demonstrate how text mining data can be incorporated into a variety of applications and research projects, covering not only human biology but also (other) animals, plants, bacteria, fungi and viruses. To ensure high quality of the textual data, the second day of the workshop will focus on manual evaluation and curation of the automatically retrieved information. Data submission, annotation and curation Natural language processing Life Science Researchers PhD students post-docs 2016-04-22 2017-04-14
Electronic Lab Notebook Introduction - Leuven

The Training consists of two parts: Introduction Access ways to your notebook, ELN installation instructions and house rules, where to find documentation and get support Hands-on-demo Creating notebooks/experiments, importing and exporting (MS Office) files, sharing data, using templates,...

Electronic Lab Notebook Introduction - Leuven https://tess.elixir-europe.org/materials/electronic-lab-notebook-introduction-leuven The Training consists of two parts: Introduction Access ways to your notebook, ELN installation instructions and house rules, where to find documentation and get support Hands-on-demo Creating notebooks/experiments, importing and exporting (MS Office) files, sharing data, using templates, advanced searching, printing, tips and tricks Life Science Researchers PhD students post-docs 2016-04-22 2016-06-15
Initiation GIMP and Inkscape - Leuven

Introduction Bitmap vs vector images Image resolution PPI and DPI File formats VIB guidelines on image editing GIMP prepare images for publication Installation and documentation User Interface Import image files Image Properties Selection tools and options Transforming...

Initiation GIMP and Inkscape - Leuven https://tess.elixir-europe.org/materials/initiation-gimp-and-inkscape-leuven Introduction Bitmap vs vector images Image resolution PPI and DPI File formats VIB guidelines on image editing GIMP prepare images for publication Installation and documentation User Interface Import image files Image Properties Selection tools and options Transforming options Scaling and cropping Using Layers Contrast and brightness Undo/redo Image print size Saving/Exporting images Exercises Inkscape annotate plots and images, create figures (diagrams, schemes, ...), design posters Installation and documentation User interface Import image/vector files Drawing lines Creating paths Drawing objects Fill and stroke Aligning and distributing objects/nodes Z-order Adding text and text options Path effects Live path effects Diagrams Exporting images Exercises Life Science Researchers PhD students post-docs 2016-04-22 2016-06-15
Custom training: Ingenuity Pathway Analysis

The aim of this training is to teach you how to use Ingenuity Pathway Analysis (IPA) software on your own data. This can be for various applications: Creating and modifying biological networks e.g. which genes are involved in a certain disease Mapping data to biological processes,...

Scientific topics: Microarray experiment, Proteomics, Protein interactions, Metabolomics

Custom training: Ingenuity Pathway Analysis https://tess.elixir-europe.org/materials/custom-training-ingenuity-pathway-analysis The aim of this training is to teach you how to use Ingenuity Pathway Analysis (IPA) software on your own data. This can be for various applications: Creating and modifying biological networks e.g. which genes are involved in a certain disease Mapping data to biological processes, networks and pathways mapping a list of genes/proteins/chemicals mapping quantitative data e.g. microarray/proteomics/metabolomics data High quality visualisation of biological networks and pathways Assessing the biological significance of pathways in certain physiological conditions e.g. detecting overrepresented pathways in diseases Microarray experiment Proteomics Protein interactions Metabolomics Life Science Researchers post-docs PhD students 2016-04-22 2016-09-26
Metagenomics

This training will start with the presentation of a 16S pipeline (psbweb05.psb.ugent.be/lotus/) in a linux environment, using a minimal amount of linux commands. This will enable the preprocessing of the data going from raw reads to taxonomic tables and phylogenetic trees. The 2nd part of the...

Metagenomics https://tess.elixir-europe.org/materials/metagenomics This training will start with the presentation of a 16S pipeline (psbweb05.psb.ugent.be/lotus/) in a linux environment, using a minimal amount of linux commands. This will enable the preprocessing of the data going from raw reads to taxonomic tables and phylogenetic trees. The 2nd part of the training will give an overview of numerical ecology and takes part entierly in R. post-docs PhD students beginner bioinformaticians Life Science Researchers 2016-04-22 2016-08-31
Electronic Lab Notebook Introduction - Ghent

The Training consists of two parts: Introduction Access ways to your notebook, ELN installation instructions and house rules, where to find documentation and get support Hands-on-demo Creating notebooks/experiments, importing and exporting (MS Office) files, sharing data, using templates,...

Electronic Lab Notebook Introduction - Ghent https://tess.elixir-europe.org/materials/electronic-lab-notebook-introduction The Training consists of two parts: Introduction Access ways to your notebook, ELN installation instructions and house rules, where to find documentation and get support Hands-on-demo Creating notebooks/experiments, importing and exporting (MS Office) files, sharing data, using templates, advanced searching, printing, tips and tricks Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Custom training: OpenRefine

The aim of this training is to help you use OpenRefine on your own data. This can contain subjects like: load data files, import and parse data files from a URL clean data files: rename columns, transform and edit cells, split multivalue cells... transform data from one format to another:...

Scientific topics: Data quality management

Custom training: OpenRefine https://tess.elixir-europe.org/materials/custom-training-openrefine The aim of this training is to help you use OpenRefine on your own data. This can contain subjects like: load data files, import and parse data files from a URL clean data files: rename columns, transform and edit cells, split multivalue cells... transform data from one format to another: e.g. merge data from two files using  a column that is common to both data files, create new rows based on columns and vice versa... export cleaned, transformed data data manipulation pipelines data dashboards Data quality management Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Basic bioinformatics concepts, databases and tools

During this 3-day introduction to bioinformatics, BITS will take you along a guided tour that covers all basic aspects of biological sequence analysis. This workshop is an ideal opportunity to get familiar with bioinformatics and its many faces! At the end, you should be able to start tackling...

Scientific topics: Sequence analysis

Basic bioinformatics concepts, databases and tools https://tess.elixir-europe.org/materials/basic-bioinformatics-concepts-databases-and-tools During this 3-day introduction to bioinformatics, BITS will take you along a guided tour that covers all basic aspects of biological sequence analysis. This workshop is an ideal opportunity to get familiar with bioinformatics and its many faces! At the end, you should be able to start tackling any basic bioinformatics problem: how to obtain relevant information about your gene, you know where to get the required sequence data, manipulate the sequences, and how basic software and algorithms function so you can fine-tune it in order the get the most out of your analysis. Sequence analysis Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Basics of databases and MySQL

This hands-on training provides a comprehensive introduction to relational databases and the use of the MySQL relational database management system. MySQL is the leading free management system for relational databases. The training shows you how to use MySQL to efficiently store your data in...

Basics of databases and MySQL https://tess.elixir-europe.org/materials/basics-of-databases-and-mysql This hands-on training provides a comprehensive introduction to relational databases and the use of the MySQL relational database management system. MySQL is the leading free management system for relational databases. The training shows you how to use MySQL to efficiently store your data in databases and manage both data and access to the data. The training gives an overview of the SQL syntax that allows you to access and manipulate the data. Additionally, the use of Python to automatically access MySQL databases is explained. Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22 2017-10-09
Introduction to the analysis of NGS data

This training is a prerequisite introduction to a series of trainings on the analysis of NGS data for different applications: 'Hands-on introduction to NGS variant analysis', 'RNA-Seq analysis for differential expression', and 'Hands-on introduction to ChIP-Seq analysis'. If you want to follow...

Scientific topics: Workflows, Sequence assembly

Introduction to the analysis of NGS data https://tess.elixir-europe.org/materials/introduction-to-the-analysis-of-ngs-data This training is a prerequisite introduction to a series of trainings on the analysis of NGS data for different applications: 'Hands-on introduction to NGS variant analysis', 'RNA-Seq analysis for differential expression', and 'Hands-on introduction to ChIP-Seq analysis'. If you want to follow one of these trainings, you have to follow this introduction. The training will give you all the background that you will need in the later NGS trainings. Workflows Sequence assembly Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Basic statistics in R

This training gives an introduction to the use of the statistical software language R. R is a language for data analysis and graphics. This introduction to R is aimed at beginners. The training covers data handling, graphics, mathematical functions and some statistical techniques. R is for free...

Keywords: Statistical calculation

Basic statistics in R https://tess.elixir-europe.org/materials/basic-statistics-in-r This training gives an introduction to the use of the statistical software language R. R is a language for data analysis and graphics. This introduction to R is aimed at beginners. The training covers data handling, graphics, mathematical functions and some statistical techniques. R is for free and for more information you can visit the site at the CRAN web site. This training is an introduction to the use of R and RStudio and stops at very basic analyses (t-tests and non-parametric equivalents). A full overview of statistical analyses in R including regression, ANOVA will be given in the follow-up training Basic statistics in R, part II Statistical calculation Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Introduction to CLC Bio Main Workbench

In this hands-on training you will learn how to use the CLC Bio Main Workbench for basic and advanced sequence analysis. The software supports a large number of DNA, RNA, and protein sequence analyses and microarray analysis. Since BITS provides a VIB-wide license to CLC Bio Main Workbench, this...

Scientific topics: Expression data visualisation, Local alignment, Global alignment

Introduction to CLC Bio Main Workbench https://tess.elixir-europe.org/materials/introduction-to-clc-bio-main-workbench In this hands-on training you will learn how to use the CLC Bio Main Workbench for basic and advanced sequence analysis. The software supports a large number of DNA, RNA, and protein sequence analyses and microarray analysis. Since BITS provides a VIB-wide license to CLC Bio Main Workbench, this training is targeted specifically to VIB scientists. Expression data visualisation Local alignment Global alignment Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
UCSC Genome Browser

The UCSC Genome Browser provides a way to examine data from many genomes and includes extensive annotation tracks for various data types such as known UCSC (and Ensembl) genes, predicted genes, SNPs, miRNAs, comparative multi-species analysis and much more. During this training you will learn how...

UCSC Genome Browser https://tess.elixir-europe.org/materials/ucsc-genome-browser The UCSC Genome Browser provides a way to examine data from many genomes and includes extensive annotation tracks for various data types such as known UCSC (and Ensembl) genes, predicted genes, SNPs, miRNAs, comparative multi-species analysis and much more. During this training you will learn how to navigate this vital tool for your research. Required skills No specific prior knowledge is required.  BITS provides 15 laptops for their training sessions. Depending on the number of participants (max 20), it is possible that you have to share the laptop with one other participant but you can also choose to bring your own laptop for this training session. Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Protein Structure Analysis

This training session will provide the basics of protein structure determination and how this information is stored in databases. We will explore and search in online databases containing protein structure information. With the aid of the Yasara View program we will visualize the structure....

Scientific topics: Protein structure analysis

Protein Structure Analysis https://tess.elixir-europe.org/materials/protein-structure-analysis This training session will provide the basics of protein structure determination and how this information is stored in databases. We will explore and search in online databases containing protein structure information. With the aid of the Yasara View program we will visualize the structure. Different hands-on exercises will allow you to compare the structure of homologues, to predict a structural model of proteins without any structure information and to find homologuous structures. We will use the FoldX program to quantify various interactions in the structures. Protein structure analysis Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
GATK Best practices for variant discovery

ÔÇïIn the course of this workshop, we highlight key functionalities such as  GVCF workflow for joint variant discovery in cohorts RNAseq-specific processing somatic variant discovery using MuTect2.  capabilities of the upcoming GATK version 4, including a new workflow for CNV discovery the use...

Scientific topics: RNA-Seq

GATK Best practices for variant discovery https://tess.elixir-europe.org/materials/gatk-best-practices-for-variant-discovery ÔÇïIn the course of this workshop, we highlight key functionalities such as  GVCF workflow for joint variant discovery in cohorts RNAseq-specific processing somatic variant discovery using MuTect2.  capabilities of the upcoming GATK version 4, including a new workflow for CNV discovery the use of pipelining tools to assemble and execute GATK workflows. RNA-Seq Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22 2017-10-09
Analysis of qPCR data using qbase+

This hands-on training provides an introduction to the analysis of qPCR data using Biogazelle's qbase+ software. Although this software allows to do various types of analyses (gene expression, copy number analysis, miRNA profiling), we will focus in the training on gene expression analysis....

Scientific topics: PCR experiment, Gene expression analysis, Gene expression

Analysis of qPCR data using qbase+ https://tess.elixir-europe.org/materials/analysis-of-qpcr-data-using-qbase This hands-on training provides an introduction to the analysis of qPCR data using Biogazelle's qbase+ software. Although this software allows to do various types of analyses (gene expression, copy number analysis, miRNA profiling), we will focus in the training on gene expression analysis. qbase+ software is freely distributed by BITS to VIB scientists. Therefore the training will be limited to VIB scientists. PCR experiment Gene expression analysis Gene expression Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Introduction to Affymetrix microarray analysis

Following this training is a prerequisite to the training on the analysis of Affymetrix microarray data using free Affymetrix software. If you want to follow this training, you have to follow this introduction first. If you want to follow the Genevestigator training or the training on the...

Scientific topics: Expression data visualisation, Gene expression

Introduction to Affymetrix microarray analysis https://tess.elixir-europe.org/materials/introduction-to-affymetrix-microarray-analysis Following this training is a prerequisite to the training on the analysis of Affymetrix microarray data using free Affymetrix software. If you want to follow this training, you have to follow this introduction first. If you want to follow the Genevestigator training or the training on the analysis of public microarray data sets and you have no experience with microarrays, you can also follow this introduction or consult the training material of this introduction. Expression data visualisation Gene expression Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Basic statistics theory

This training gives an introduction to the use of statistics for basic analyses of life sciences data. This training is a prerequisite introduction to a series of hands-on trainings on the statistical analysis of life sciences data: 'Basic statistics in R' and 'Basic statistics in Graphpad...

Basic statistics theory https://tess.elixir-europe.org/materials/basic-statistics-theory This training gives an introduction to the use of statistics for basic analyses of life sciences data. This training is a prerequisite introduction to a series of hands-on trainings on the statistical analysis of life sciences data: 'Basic statistics in R' and 'Basic statistics in Graphpad Prism'. If you want to follow one of these trainings, you have to follow this introduction. Ít will give you all the theoretical background that you need. Those that already have a strong statistical background may register directly for the 'Basic statistics in R, part II' training. Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Introduction to gene regulation

This course presents a comprehensive overview of the principles and applications of studying eukaryotic gene regulation. The course combines lectures with hands-on exercises in which the participants learn to apply these principles to real data. The course will be based on freely available data...

Scientific topics: Molecular biology, Gene regulation, Gene expression

Introduction to gene regulation https://tess.elixir-europe.org/materials/introduction-to-gene-regulation This course presents a comprehensive overview of the principles and applications of studying eukaryotic gene regulation. The course combines lectures with hands-on exercises in which the participants learn to apply these principles to real data. The course will be based on freely available data and software. Molecular biology Gene regulation Gene expression Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Analysis of public microarray data using Genevestigator

The morning session will give you an overview of the possibilities of Genevestigator, a search engine of curated and normalized publicly available gene expression data. The interface allows you to visualize spatio-temporal (tissue - developmental stage) and perturbational gene expression...

Scientific topics: Gene expression analysis, Gene expression, Biomarkers

Analysis of public microarray data using Genevestigator https://tess.elixir-europe.org/materials/analysis-of-public-microarray-data-using-genevestigator The morning session will give you an overview of the possibilities of Genevestigator, a search engine of curated and normalized publicly available gene expression data. The interface allows you to visualize spatio-temporal (tissue - developmental stage) and perturbational gene expression information of many species in a user-friendly graphical way. The afternoon session will focus on the practical applications in a hands-on tutorial on Genevestigator followed by concrete examples of software that can be used to further interpret the results of your Genevestigator searches, like Ingenuity Pathway Analysis and DAVID. Finally, you will be able to work on the questions that you want to answer in your own research under the supervision of the trainer. Participants are therefore encouraged to submit a description of the objectives of the analysis they want to perform. Gene expression analysis Gene expression Biomarkers Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Gentle hands-on introduction to Perl programming

This course is organised over two full days. With this extensive hands-on session, we introduce you Perl. The first day,we will cover the basics of Perl programming: what is a variable, different types, how to assign values to variable, what can you do with them, strings, arrays and hashes. Logic...

Gentle hands-on introduction to Perl programming https://tess.elixir-europe.org/materials/gentle-hands-on-introduction-to-perl-programming This course is organised over two full days. With this extensive hands-on session, we introduce you Perl. The first day,we will cover the basics of Perl programming: what is a variable, different types, how to assign values to variable, what can you do with them, strings, arrays and hashes. Logic statements will be discussed, Everything will be demonstrated with step by step exercises. At the end of the day, you will be send home with some challenges to do yourself and additional learning material. As a homework, you may write a program to analyze your own data. On the second day of the training, we will look to the solutions of the homework and explain the results of the tasks. We will dig a little deeper in good programming hygiene, and create some more complex scripts using regexes and reading in files. By the end of the day, a firm basis for perl programming should be established to allow further self-study. 2016-04-22 2017-10-09
Gentle hands-on introduction to Python programming

This course is organised over two full days. With the help of plenty hands-on exercises, you will get introduced into the different types of variables in python, the peculiarities of python and good programming habits. This course will provide you an ideal stepping stone for further developing...

Scientific topics: Software engineering

Gentle hands-on introduction to Python programming https://tess.elixir-europe.org/materials/gentle-hands-on-introduction-to-python-programming This course is organised over two full days. With the help of plenty hands-on exercises, you will get introduced into the different types of variables in python, the peculiarities of python and good programming habits. This course will provide you an ideal stepping stone for further developing programming skills in Python. Software engineering Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22 2017-10-09
Introduction to Linux for bioinformatics

This training is intended for all Linux novices who want to learn the basics of Linux without getting too much technical details. This hands-on training will show you how to effectively use Linux, a free operating system. Bioinformatics depends heavily on Linux-based computers and software. A lot...

Introduction to Linux for bioinformatics https://tess.elixir-europe.org/materials/introduction-to-linux-for-bioinformatics This training is intended for all Linux novices who want to learn the basics of Linux without getting too much technical details. This hands-on training will show you how to effectively use Linux, a free operating system. Bioinformatics depends heavily on Linux-based computers and software. A lot of good scientific software is written specifically for Linux/Unix. Additionally, Linux has most popular programming languages (e.g.Python, Perl, C) already installed and ready to use! Data management happens swiftly with the dedicated text manipulation tools and file system properties. This training will teach you all you need to know to swiftly start using Linux, be it on the server of your department, or on your own computer (even if you're already running Windows or Mac OS). Prior to the training session, you will receive a poll to ask you your personal goals by following this training sesion. Depending on the outcome, the schedule will be adjusted. Life Science Researchers PhD students beginner bioinformaticians 2016-04-22 2017-10-09
Basic statistics in R, part II

This training builds further on the Basic statistics in R training, showing you how to do statistical analyses in R. Where the Basic statistics in R training is an introduction to the use of R and RStudio and stops at very basic analyses (t-tests and non-parametric equivalents), this training...

Basic statistics in R, part II https://tess.elixir-europe.org/materials/basic-statistics-in-r-part-ii This training builds further on the Basic statistics in R training, showing you how to do statistical analyses in R. Where the Basic statistics in R training is an introduction to the use of R and RStudio and stops at very basic analyses (t-tests and non-parametric equivalents), this training gives you a full overview of statistical analyses in R including regression, ANOVA... Emphasis will be placed on practical applications. To this end, theory will be complemented with hands-on exercises in R, a free software environment for statistical computing and graphics. Topics include distributions, plots, confidence intervals, hypothesis testing... Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22 2017-10-09
Analyzing flow cytometry data using FlowJo

Flowjo version10 is a newly designed version of the famous Flowjo software. In contrast with previous versions (iOS only), version 10 is now a multi-platform software tool (Windows, iOS, Linux). It now has a very intuitive user interface designed to analyze your data in a very practical and fast...

Analyzing flow cytometry data using FlowJo https://tess.elixir-europe.org/materials/analyzing-flow-cytometry-data-using-flowjo Flowjo version10 is a newly designed version of the famous Flowjo software. In contrast with previous versions (iOS only), version 10 is now a multi-platform software tool (Windows, iOS, Linux). It now has a very intuitive user interface designed to analyze your data in a very practical and fast way. The training will be focused on getting familiar with all the tools available within the software. Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Hands-on introduction to NGS variant analysis

This training gives an introduction to the use of popular NGS analysis software packages at the command line. It reviews several exchangeable tools and provide hints to evaluate quality and content of DNA-Seq data by a biologist.   This training does not cover all methods available today. It does...

Scientific topics: DNA

Hands-on introduction to NGS variant analysis https://tess.elixir-europe.org/materials/hands-on-introduction-to-ngs-variant-analysis This training gives an introduction to the use of popular NGS analysis software packages at the command line. It reviews several exchangeable tools and provide hints to evaluate quality and content of DNA-Seq data by a biologist.   This training does not cover all methods available today. It does not aim at bringing users to a professional NGS analyst level but provides enough information to allow biologists understand what DNA sequencing practically is and to communicate with NGS experts for more in-depth needs. DNA Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22 2017-10-09
Initiation GIMP and Inkscape

Introduction Bitmap vs vector images Image resolution PPI and DPI File formats and compression VIB guidelines on image editing GIMP prepare images for publication Installation and documentation User Interface Import image files Image Properties Selection tools and options Transforming...

Initiation GIMP and Inkscape https://tess.elixir-europe.org/materials/initiation-gimp-and-inkscape-ghent Introduction Bitmap vs vector images Image resolution PPI and DPI File formats and compression VIB guidelines on image editing GIMP prepare images for publication Installation and documentation User Interface Import image files Image Properties Selection tools and options Transforming options Scaling and cropping Using Layers Contrast and brightness Undo/redo Image print size Saving/Exporting images Exercises Inkscape annotate plots and images, create figures (diagrams, schemes, ...), design posters Installation and documentation User interface Import image/vector files Drawing lines Creating paths Drawing objects Fill and stroke Aligning and distributing objects/nodes Z-order Adding text and text options Path effects Live path effects Diagrams Exporting images Exercises 2017-10-09
Analysis of metabolome data

This training will focus on the processing of raw data obtained via either Gas Chromatography- (GC) or Liquid Chromatography- (LC) mass spectrometry (MS). In addition, based on the comparative analysis between two sample sets (e.g. control vs treatment), the subsequent identification of...

Scientific topics: Metabolomics

Analysis of metabolome data https://tess.elixir-europe.org/materials/analysis-of-metabolome-data This training will focus on the processing of raw data obtained via either Gas Chromatography- (GC) or Liquid Chromatography- (LC) mass spectrometry (MS). In addition, based on the comparative analysis between two sample sets (e.g. control vs treatment), the subsequent identification of differential metabolites will be introduced. Afterwards, the trainee should be able to perform independently a comparative analysis of raw metabolome data and to pinpoint the well-known metabolites in the chromatogram. Metabolomics Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22
Image Ethics and Poster Design

The image ethics part handles on the theoretical aspects of image manipulation, the mistakes people make and how to avoid image fraud. VIB has guidelines for acceptable scientific image manipulations. Not all manipulations are scientifically correct. There are manipulations that fall within the...

Scientific topics: Image

Image Ethics and Poster Design https://tess.elixir-europe.org/materials/image-ethics-and-poster-design The image ethics part handles on the theoretical aspects of image manipulation, the mistakes people make and how to avoid image fraud. VIB has guidelines for acceptable scientific image manipulations. Not all manipulations are scientifically correct. There are manipulations that fall within the scope of scientific misconduct, because they result in misrepresentation of the data. Such misrepresentation makes it impossible for others to interpret the data correctly, or worse, leads to conclusions that are not correct. The second part of this course deals with poster design. Scientific posters are a resume of a piece or timespan of research on one square meter of paper. They are best compared to slides for oral presentations, not a written document. Posters can’t convey detailed evidence like scientific articles, it must visualize a message on its own. When standing a meter away from a poster you hardly feel like reading much text, especially when the author/designer stands next to it. A poster must bring that message without requiring oral explanation, but with as little text as possible. Prerequisites Participants must have experience with GIMP and Inkscape. Schedule See the TRAINING AT VIB website for a detailed schedule of this training. Training material Not available Links Not available Scientific topics Image data Target audience Life Science Researchers, PhD students, post-docs, beginner bioinformaticians   Image Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22
Probabilistic programming with (R)Stan

Probabilistic models describe how the observed data was generated, and what structure the signal and noise from potentially multiple sources may have. Many classical statistical models are special cases of probabilistic models with special modeling assumptions. Probabilistic models can be...

Scientific topics: Statistics and probability

Probabilistic programming with (R)Stan https://tess.elixir-europe.org/materials/probabilistic-programming-with-r-stan Probabilistic models describe how the observed data was generated, and what structure the signal and noise from potentially multiple sources may have. Many classical statistical models are special cases of probabilistic models with special modeling assumptions. Probabilistic models can be implemented, improved, and critizised in a flexible, explicit and transparent manner, and the analysis can be supported with prior information about the data. This 1-day course provides an introduction to Bayesian/probabilistic models. We will implement standard linear models based on the rstanarm package of the R statistical programming environment and readily available example data sets. The workshop is an ideal opportunity to familiarize yourself with the basic ideas in probabilistic modeling such as prior information, likelihood, model criticism and validation, as well as some of the available tools. At the end, you should be able to implement basic probabilistic models yourself, and understand their relative advantages and pitfalls compared to their classical alternatives. Statistics and probability Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22
Introduction to Biopython

Biopython is the best-known Python library to process biological data. This training is aimed to empower you to use Biopython to make your research more efficient. The first day of the training is to give an overview of Biopython. You are going to start with your first steps in Biopython on the...

Scientific topics: Software engineering

Introduction to Biopython https://tess.elixir-europe.org/materials/introduction-to-biopython-8ccf2441-bd7d-46f2-84f8-c2b123844a23 Biopython is the best-known Python library to process biological data. This training is aimed to empower you to use Biopython to make your research more efficient. The first day of the training is to give an overview of Biopython. You are going to start with your first steps in Biopython on the command line. Afterwards you will take a tour of the most important components: sequences, NCBI queries, BLAST, trees, and 3D structures. You will try each of these modules on practical examples. Please don't hesitate to ask questions about Python basics or particular data formats (e.g. XML or NGS data). The second day of the training is to broaden your perspective: What other features does the library have? How can you use the documentation effectively? What is Biopython not capable of? What can I do to visualize my data? Are there alternatives? If you have your own data that you would like to work on with Biopython in more detail, there is room for that. For us, the most important thing is to identify concrete Python modules and functions that help you to get your research done. Participants are encouraged to submit a description of their research topic and/or the questions they would like to answer with Biopython. Additionally, participants can bring their own data that they would like to process in Python to the training. Software engineering Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22 2017-10-09
Mass spectrometry data processing

In this training session we start by reviewing the types of mass spectrometer, and what the properties of their output data are. Out of our understanding of the data, we learn to extract meaningful interpretations.

Scientific topics: Proteomics

Mass spectrometry data processing https://tess.elixir-europe.org/materials/mass-spectrometry-data-processing In this training session we start by reviewing the types of mass spectrometer, and what the properties of their output data are. Out of our understanding of the data, we learn to extract meaningful interpretations. Proteomics Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
Advanced FlowJo training

The training will start with an introduction to FlowJo v10 but there will be enough details and features to make it worth even for advanced users. In the afternoon, advanced tools in FlowJo and new plugins (tSNE, SPADE) will be presented showing you how to work in high...

Advanced FlowJo training https://tess.elixir-europe.org/materials/advanced-flowjo-training The training will start with an introduction to FlowJo v10 but there will be enough details and features to make it worth even for advanced users. In the afternoon, advanced tools in FlowJo and new plugins (tSNE, SPADE) will be presented showing you how to work in high dimensionality. Prerequisites Participants must have experience with FlowJo. Schedule See the TRAINING AT VIB website for a detailed schedule of this training. Training material Not available Links Not available Scientific topics FlowJo Target audience Life Science Researchers, PhD students, post-docs, beginner bioinformaticians Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22
Introductory statistics in GraphPad Prism

This training gives an introduction to the use of statistics for basic analyses of life sciences data. The theoretical background obtained in the Basic statistics theory training  will be applied in a hands-on session on real-life data using the statistical software GraphPad...

Introductory statistics in GraphPad Prism https://tess.elixir-europe.org/materials/introductory-statistics-in-graphpad-prism This training gives an introduction to the use of statistics for basic analyses of life sciences data. The theoretical background obtained in the Basic statistics theory training  will be applied in a hands-on session on real-life data using the statistical software GraphPad Prism. BITS distributes the software for free to VIB scientists, for more information you can visit the GraphPad Prism page on the BITS web site. This is the reason why the training is limited to VIB scientists. Life Science Researchers PhD students post-docs 2016-04-22 2017-10-09
RNA-Seq analysis for differential expression in GenePattern

We will investigate the following items: Quality control of the sequence reads to detect biases or contaminating adapters Mapping of the reads to the reference genome with use of a transcript database model Quality control of the mapping results Adjusting the mapping data to...

RNA-Seq analysis for differential expression in GenePattern https://tess.elixir-europe.org/materials/rna-seq-analysis-for-differential-expression-in-genepattern We will investigate the following items: Quality control of the sequence reads to detect biases or contaminating adapters Mapping of the reads to the reference genome with use of a transcript database model Quality control of the mapping results Adjusting the mapping data to compensate for artefacts like duplicates Calculate transcript counts usable for differential expression and merging of count tables Computing differential expression using EdgeR and DESeq2 The code pieces used during the training are made available through our Wiki as well as detailed results and can be copied and adapted for own user needs with minimal edits. Key results have been saved to our server and can be downloaded to fully reproduce the training. Life Science Researchers PhD students beginner bioinformaticians post-docs 2016-04-22 2017-10-09
Showing 7 upcoming events. Found 56 past events. View all results.
  • Introduction to the analysis of NGS data

    11 January 2019

    Leuven, Belgium

    Elixir node event
    Introduction to the analysis of NGS data https://tess.elixir-europe.org/events/introduction-to-the-analysis-of-ngs-data b'\nThis training is an introduction to a series of trainings on the analysis of Next Generation Sequencing data. This training is intended for newbies to the field and will teach them all background knowledge required to successfully complete the advanced NGS analysis trainings. To this end the training will give an overview of:\r\n- The Illumina platform\r\n- NGS data formats and file handling\r\n- Quality control of NGS data\r\n- Assembly and mapping of NGS data\r\n- Supporting IT: Galaxy and GenePattern platform\r\n\r\n\n This training is an introduction to \'Hands on introduction to ChIP-Seq analysis\', Hands-on introduction to NGS variant analysis\', \'GATK training\', and \'RNA-Seq analysis for differential expression in GenePattern\'. If you want to follow one of these trainings but you have no experience with NGS data you should follow this introduction. \n\r\n\r\n\n' 2019-01-11 09:30:00 UTC 2019-01-11 17:00:00 UTC VIB Bioinformatics Core Park Inn by Radisson Leuven, Leuven, Belgium Park Inn by Radisson Leuven Leuven Belgium 3010 [] [] [] [] [] []
  • Analyzing flow cytometry data in FlowJo

    25 February 2019

    Ghent (Sint-Denijs-Westrem), Belgium

    Elixir node event
    Analyzing flow cytometry data in FlowJo https://tess.elixir-europe.org/events/analyzing-flow-cytometry-data-in-flowjo b'Learn researchers how to analyze flow cytometry data in an efficient way using FlowJo v10,the world’s nr.1 analysis software tool for flow cytometry data.\r\n\n FlowJo version10 is a newly designed version of the famous FlowJo software. In contrast with previous versions (iOS only), version 10 is now a multi-platform software tool (Windows, iOS, Linux). It now has a very intuitive user interface designed to analyze your data in a very practical and fast way.\r\n\n Prior knowledge in general flow cytometry is required but the general principles will be repeated\r\n\n' 2019-02-25 09:30:00 UTC 2019-02-25 17:00:00 UTC VIB Bioinformatics Core Holiday Inn Gent Expo, Ghent (Sint-Denijs-Westrem), Belgium Holiday Inn Gent Expo Ghent (Sint-Denijs-Westrem) Belgium 9051 [] [] [] [] [] []
  • Introductory statistics in GraphPad Prism

    18 December 2018

    Leuven, Belgium

    Elixir node event
    Introductory statistics in GraphPad Prism https://tess.elixir-europe.org/events/introductory-statistics-in-graphpad-prism b'In this hands-on session, you will apply the theory that you learnt in the Basic statistics theory training in Prism. The following topics will be covered:\nWhat is Prism? Where to find more information? How to install and use it?\r\nHow to use Prism to handle data: reading data\r\nHow to use Prism to create graphics\r\nHow to use basic statistical techniques in Prism: mean, variance, confidence intervals, correlations, hypothesis testing...\n\n\r\n\n  \r\n\n  \r\n\n' 2018-12-18 09:30:00 UTC 2018-12-18 17:00:00 UTC VIB Bioinformatics Core Park Inn by Radisson Leuven, Leuven, Belgium Park Inn by Radisson Leuven Leuven Belgium 3010 [] [] [] [] [] []
  • Basic statistics in R

    7 - 21 January 2019

    Leuven, Belgium

    Elixir node event
    Basic statistics in R https://tess.elixir-europe.org/events/basic-statistics-in-r b'\n\nGet an idea of what R and Rstudio is\r\nUse R to handle data: creating, reading, reformatting and writing data\r\nUse R to create graphics\r\nUse basic statistical techniques in R : normality tests, t-tests, wilcoxon tests, chi square tests, correlations, survival analysis...\r\nWrite and use R scripts\n\r\n\r\n\n \nThis training gives an introduction to the use of the statistical software language R. R is a language for data analysis and graphics. This introduction to R is aimed at beginners. The training covers data handling, graphics, and basic statistical techniques. \r\nR is for free and for more information you can visit the site the CRAN web site.This\r\n training is an introduction to the use of R and RStudio and stops at very basic \r\nanalyses (t-tests and non-parametric equivalents). A full overview of statistical analyses in R including regression, \r\nANOVA will be given in the follow-up training Basic statistics in R, part II.\n\r\n \r\nRequirements\r\nThe training is intended for people who have no experience with R. However, understanding of basic statistical concepts is required, such as data types, normal distribution, descriptive statistics, tests for comparing groups... If you don\'t have sufficient statistical background you are strongly encouraged to attend the Basic statistics theory training. \r\nThis training is recommended for people with no experience in R who are planning to follow a training that requires some R background, like the mass spectrometry training, the single cell RNA-Seq and the bulk RNA-Seq training....\n\r\n\n' 2019-01-07 09:30:00 UTC 2019-01-21 17:00:00 UTC VIB Bioinformatics Core Park Inn by Radisson Leuven, Leuven, Belgium Park Inn by Radisson Leuven Leuven Belgium 3010 [] [] [] [] [] []
  • Using MOFA for integration of omics data

    3 May 2019

    Leuven, Belgium

    Elixir node event
    Using MOFA for integration of omics data https://tess.elixir-europe.org/events/using-mofa-for-integration-of-omics-data 2019-05-03 09:30:00 UTC 2019-05-03 17:00:00 UTC VIB Bioinformatics Core Park Inn by Radisson Leuven, Leuven, Belgium Park Inn by Radisson Leuven Leuven Belgium 3010 [] [] [] [] [] []
  • Basic statistics in R, Ghent

    15 - 29 January 2019

    Ghent, Belgium

    Elixir node event
    Basic statistics in R, Ghent https://tess.elixir-europe.org/events/basic-statistics-in-r-ghent b'\nGet an idea of what R and Rstudio is\r\nUse R to handle data: creating, reading, reformatting and writing data\r\nUse R to create graphics\r\nUse basic statistical techniques in R : normality tests, t-tests, wilcoxon tests, chi square tests, correlations, survival analysis...\r\nWrite and use R scripts\n\r\n\n \nThis training gives an introduction to the use of the statistical software language R. R is a language for data analysis and graphics. This introduction to R is aimed at beginners. The training covers data handling, graphics, and basic statistical techniques. \r\nR is for free and for more information you can visit the site the CRAN web site.This training is an introduction to the use of R and RStudio and stops at very basic analyses (t-tests and non-parametric equivalents). A full overview of statistical analyses in R including regression, ANOVA will be given in the follow-up training Basic statistics in R, part II.\n\r\n \r\nRequirements\r\nThe training is intended for people who have no experience with R. However, understanding of basic statistical concepts is required, such as data types, normal distribution, descriptive statistics, tests for comparing groups... If you don\'t have sufficient statistical background you are strongly encouraged to attend the Basic statistics theorytraining. \r\nThis training is recommended for people with no experience in R who are planning to follow a training that requires some R background, like the mass spectrometry , the single cell RNA-Seq and the bulk RNA-Seq training....\n\r\n\n' 2019-01-15 09:30:00 UTC 2019-01-29 17:00:00 UTC VIB Bioinformatics Core Bio-Accelerator, Ghent, Belgium Bio-Accelerator Ghent Belgium 9052 [] [] [] [] [] []
  • RNA-Seq analysis for differential expression

    11 - 18 February 2019

    Leuven, Belgium

    Elixir node event
    RNA-Seq analysis for differential expression https://tess.elixir-europe.org/events/rna-seq-analysis-for-differential-expression-in-genepattern b'You will execute a complete analysis workflow in GenePattern, Galaxy or command line and R to detect differential expression between two conditions\n\r\n\r\n\n We\'ll go through the different steps of the workflow:\r\n\n \nQuality control of the sequence reads to detect biases or contaminating adapters.\r\nMapping of the reads to the reference genome with use of a transcript database model.\r\nQuality control  of the mapping results.\r\nAdjusting the mapping data to compensate for artefacts like duplicates.\r\nCalculate transcript counts usable for differential expression and merging of count tables \n\r\nComputing differential expression using  DESeq2.\n\r\n\r\n\n • fastQC • trimmomatic• Groomer • STAR• samtools• Picard • RSeQC• HTSeq• R - RStudio - Bioconductor - various packages \n\r\n\r\n\n  \r\nFamiliarity with \n \nRNA-seq assembly\r\nRNA-seq analysis for isoform detection\r\nRNA-seq analysis for detection of short RNA species\n\r\n\n \n.Organised by the VIB Bioinformatics Core​\n\r\n\n' 2019-02-11 09:30:00 UTC 2019-02-18 17:00:00 UTC VIB Bioinformatics Core Park Inn by Radisson Leuven, Leuven, Belgium Park Inn by Radisson Leuven Leuven Belgium 3010 [] [] [] [] [] []