online tutorial

Analysis of single-cell ATAC-seq data

training material for Single Cell ATACseq (for course https://training.vib.be/all-trainings/analysis-single-cell-atac-seq-data-0)

This training introduces participants to scATAC-seq (single-cell Assay for Transposase-Accessible Chromatin using sequencing), a technique that reveals chromatin accessibility with cell-type resolution. The course will guide you through the analysis of scATAC-seq data in R, using ArchR, and it will also discuss the results obtained by CellRanger-ATAC. By the end of the training, participants will be able to process their own scATAC-seq datasets, and integrate results with scRNA-seq data to explore mechanisms of gene regulation.

Keywords: ATAC-seq, ArchR

Target audience: Life scientists

Resource type: online tutorial

Version: 1.0

Learning objectives:

Describe the principles and workflow of scATAC-seq technology and scATAC-seq analysis
Interpret output files and quality reports generated by CellRanger-ATAC
Apply ArchR to perform quality control, doublet inference, clustering, annotation, and peak calling of scATAC-seq datasets
Visualize the data in a Genome Browser
Integrate scATAC-seq data with scRNA-seq data to infer gene regulatory networks
Possibility to analyse your own scATAC-seq data under the guidance of experts

Date created: 2025-12-18

Authors: Vanessa Vermeirssen, Vittoria Zanzani

Contributors: VIB


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