Understanding genomic variation is essential for uncovering the genetic basis of disease, evolution, and phenotypic traits. While short-read sequencing has long been the standard, long-read technologies now offer superior resolution for detecting structural variants and phasing complex regions. This course is designed for researchers working with genomic data who want to explore the potential of long-read sequencing in variant analysis. Participants will gain hands-on experience using the GenomeComb package (https://derijkp.github.io/genomecomb/) to process long-read (and short-read) data, perform variant and structural variant calling, and annotate results. By the end of the training, attendees will be equipped to apply these methods to their own datasets and interpret the biological relevance of the findings.