This course provides hands-on training in the basics of mass spectrometry (MS) and proteomics bioinformatics. You will receive training on how to use search engines and post-processing software, quantitative approaches, MS data repositories, the use of public databases for protein analysis, annotation of subsequent protein lists, and incorporation of information from molecular interaction and pathway databases. The practical elements of the course will take raw data from a proteomics experiment and analyse it. You will be able to go from MS aspectra to identifying and quantifying peptides, and finally obtaining lists of protein identifiers that can be analysed further using a wide range of resources. The final aim is to provide you with the practical bioinformatics knowledge you need to go back to the lab and process your data when collected.