This two-day training session will provide the basics of protein structure analysis and how this information is stored in databases.On the first day, we will explore and search in online databases containing protein structure information including models from AlphaFoldDB. Furthermore, we will visualize protein structures using ChimeraX. Different hands-on exercises will allow you to compare the structure of homologues. We will use (online) tools like MutateX on the HPC or PROSS to quantify various interactions in the structures.The second day, we will explore different methods to access structure predictions, covering the most relevant online prediction tools (AlphaFold3, Chai-1) and installations on the HPC at the VSC UGhent (AlphaFold2, Boltz-1, AlphaPulldown)In the afternoon, the second day is dedicated to your own research question you might want to address via structural predictions with any of the provided tools. Additionally, we will learn to use AlphaPulldown, a Python package that streamlines protein-protein interaction screens.