In this workshop, we address how to predict features of proteins from their sequence only, using Python scripts to access APIs of web services available online. We will introduce single-sequence based predictions of biophysical characteristics of proteins (e.g. DynaMine for backbone dynamics), methods that use multiple sequence alignments (e.g. I-TASSER), and methods that determine the difference between a protein and its mutant form (e.g. DEOGEN2). We will also show how the results of these predictions can be combined and analysed using a protein case study.