GOBLET

GOBLET, the Global Organisation for Bioinformatics Learning, Education and Training, is a legally registered foundation providing a global, sustainable support and networking structure for bioinformatics educators/trainers and students/trainees.

GOBLET https://tess.elixir-europe.org/content_providers/goblet GOBLET, the Global Organisation for Bioinformatics Learning, Education and Training, is a legally registered foundation providing a global, sustainable support and networking structure for bioinformatics educators/trainers and students/trainees. /system/content_providers/images/000/000/004/original/logo_goblet_trans.png?1469458222
Showing 108 materials out of 108. View all results.
Introduction to Unix

Introductory lecture to the Unix/Linux command-line Description of the computer shell and the command-line interface Differences between graphical and command-line interfaces The most commond Unix/Linux commands are provided    

Keywords: Bioinformatics, Programming, Unixlinux

Introduction to Unix https://tess.elixir-europe.org/materials/introduction-to-unix Introductory lecture to the Unix/Linux command-line Description of the computer shell and the command-line interface Differences between graphical and command-line interfaces The most commond Unix/Linux commands are provided     Bioinformatics, Programming, Unixlinux Anyone wants to start using the Unix/Linux OS 2013-07-04 2017-10-09
eBioKit

Extensive teaching experience gained by conducting bioinformatics training courses in Kenya, Uganda, Mauritius (UoM), SriLanka, Sweden, Chile and Zimbabwe showed that it was difficult to successfully teach and demonstrate several bioinformatics resources, due to and often limited by slow Internet...

Keywords: Advanced bioinformatics training, Introduction bioinformatics

eBioKit https://tess.elixir-europe.org/materials/ebiokit Extensive teaching experience gained by conducting bioinformatics training courses in Kenya, Uganda, Mauritius (UoM), SriLanka, Sweden, Chile and Zimbabwe showed that it was difficult to successfully teach and demonstrate several bioinformatics resources, due to and often limited by slow Internet access. For that reason a bioinformatics platform, eBioKit  was engineered to ease the administrative burden of regularly updating large databases and installing software. This platform contains more than 300 bioinformatics applications (EMBOSS, Galaxy, Blast, RSAT etc),  and most relevant databases (ENSEMBL, Uniprot, OMIM, PDB, etc) locally, solving the network speed related problems and problems associated with the installation of software. Version 2 of this system has been successfully tested in real world situations both for capacity building and research in Kenya at ILRI, the Biosciences eastern and central Africa (BecA), KEMRI Wellcome Trust Research Programme (KWTRP) and The International Centre of Insect Physiology and Ecology (ICIPE). The system has further been deployed in the SANBio bioinformatics network in Southern Africa (10 countries) and Sri Lanka (Version 1) and has recently been adopted by the H3ABioNet African Bioinformatics Network as a platform to provide bioinformatics training and bioinformatics computing services at nodes in the network (http://www.h3abionet.org).  Advanced bioinformatics training, Introduction bioinformatics Bachelor students Bench biologists Life Science Researchers PhD students 2013-10-03 2017-10-09
Introduction to Bioinformatics

An introduction to bioinformatics for bench biologists delivered as part of the EMBL Australia Masterclass on Protein Sequence Analysis http://oz-masterclass.wikispaces.com/ . Focuses on using UniProt to explore different reasons why information inferred by "direct assay" and "prediction" could...

Keywords: Introduction bioinformatics

Introduction to Bioinformatics https://tess.elixir-europe.org/materials/introduction-to-bioinformatics An introduction to bioinformatics for bench biologists delivered as part of the EMBL Australia Masterclass on Protein Sequence Analysis http://oz-masterclass.wikispaces.com/ . Focuses on using UniProt to explore different reasons why information inferred by "direct assay" and "prediction" could be wrong, and what we can do to spot it. Introduction bioinformatics Bench biologists 2013-10-23 2017-10-09
BioJS

Introduction to BioJS a standard for visualisation of biological data in JavaScript

Keywords: Biojs, Javascript, Software, Standards

BioJS https://tess.elixir-europe.org/materials/biojs-1ff5ad84-3b06-4997-bba6-e18a4dcf32c0 Introduction to BioJS a standard for visualisation of biological data in JavaScript Biojs, Javascript, Software, Standards software developers, bioinformaticians 2014-02-27 2017-10-09
Bioinformatics for Mass spectrometry analysis

This tutorial on Bioinformatics for Mass spectrometry analysis was part of a full series of tutorials that I have organised in 2009 while sitting in the Educational Committee of the European Proteomics Association (EuPA). Some information on bioinformatics tools would need to be verified, but the...

Keywords: Bioinformatics, Mass spectrometry, Protein identification

Bioinformatics for Mass spectrometry analysis https://tess.elixir-europe.org/materials/bioinformatics-for-mass-spectrometry-analysis This tutorial on Bioinformatics for Mass spectrometry analysis was part of a full series of tutorials that I have organised in 2009 while sitting in the Educational Committee of the European Proteomics Association (EuPA). Some information on bioinformatics tools would need to be verified, but the core of slides is still up to date. It introduces the basic ideas behind mass spectrometry and bioinformatics for protein identification from mass spectrometry data. Bioinformatics, Mass spectrometry, Protein identification Bench biologists PhD students Post-Doctoral Fellows Technicians bioinformaticians master 2013-10-30 2017-10-09
3-day hands-on NGS workshop

This document is the trainee exercise booklet for a 3 day hands-on Next Generation Sequencing workshop developed by Bioplatforms Australia, CSIRO and EMBL-EBI.  This workshop is aimed at bench biologists, PhD students or early career postdoctoral researchers or those new to the analysis of NGS...

Keywords: NGS, ChIPSeq, De novo genome assembly, RNASeq

3-day hands-on NGS workshop https://tess.elixir-europe.org/materials/3-day-hands-on-ngs-workshop This document is the trainee exercise booklet for a 3 day hands-on Next Generation Sequencing workshop developed by Bioplatforms Australia, CSIRO and EMBL-EBI.  This workshop is aimed at bench biologists, PhD students or early career postdoctoral researchers or those new to the analysis of NGS data and assumes no prior knowledge of bioinformatics. This course covers the following topics: data quality control, read alignment, ChIPSeq, RNASeq and de novo assembly. The workshop starts with the Intro to the shell material from Software Carpentry (http://software-carpentry.org/v4/shell/index.html).  Introductory lectures on each topic are presented before trainees move on that topic. The workshop has been delivered a number of times in different cities in Australia to date, in a roadshow style, and is designed to run on virtual machines on the Australian research cloud. Trainees then use NoMachine NX client to provide a remote desktop like connection to their own dedicated VM for the duration of of the workshop.  Various contributors made this workshop possible and they are credited in the introduction to each section. NGS, ChIPSeq, De novo genome assembly, RNASeq Bench biologists Life Science Researchers PhD students postdocs 2014-01-17 2017-10-09
Training Bioinformatics in the Cloud

I present the points of view, the challenges and advantages of developing training materials through the cloud.

Keywords: Advanced bioinformatics training, Cloud computing, Training

Training Bioinformatics in the Cloud https://tess.elixir-europe.org/materials/training-bioinformatics-in-the-cloud I present the points of view, the challenges and advantages of developing training materials through the cloud. Advanced bioinformatics training, Cloud computing, Training Bench biologists beginner bioinformaticians 2014-05-20 2017-10-09
STAO 2014 Understanding a genetic disease thanks to Bioinformatics

This workshop allows to discover several bioinformatics tools and databases (genome browser, alignment tool, BLAST, dbSNP, UniProtKB, PDB) in the context of the discovery of a rare variant leading to the production of a non functional insulin in a Norwegian family. Additional documents are ...

Keywords: Blast, Genome browsing, Introduction bioinformatics, Protein structure visualisation, Variant detection

STAO 2014 Understanding a genetic disease thanks to Bioinformatics https://tess.elixir-europe.org/materials/stao-2014-understanding-a-genetic-disease-thanks-to-bioinformatics This workshop allows to discover several bioinformatics tools and databases (genome browser, alignment tool, BLAST, dbSNP, UniProtKB, PDB) in the context of the discovery of a rare variant leading to the production of a non functional insulin in a Norwegian family. Additional documents are available: http://education.expasy.org/cours/Toronto Blast, Genome browsing, Introduction bioinformatics, Protein structure visualisation, Variant detection high school 2014-11-10 2017-10-09
RNA-seq Analysis 2014 Module 4 - Isoform discovery and alternative expression

Explore use of Cufflinks in reference annotation based transcript (RABT) assembly mode and ‘de novo’ assembly mode. Both modes require a reference genome sequence.  

Scientific topics: RNA

Keywords: Alternative expression, Transcript isoforms

RNA-seq Analysis 2014 Module 4 - Isoform discovery and alternative expression https://tess.elixir-europe.org/materials/rna-seq-analysis-2014-module-4-isoform-discovery-and-alternative-expression Explore use of Cufflinks in reference annotation based transcript (RABT) assembly mode and ‘de novo’ assembly mode. Both modes require a reference genome sequence.   RNA Alternative expression, Transcript isoforms Biologists, Genomicists, Computer Scientists Graduate Students Post-Doctoral Fellows Researchers 2014-06-27 2017-10-09
De Novo Assembly @ TGAC 2015

This course took place between 12-15 May 2015 Overview: The course will provide an introduction to de novo assembly, with a hands on introduction followed by in-depth analysis of the key steps in the process. It covers several aspects such as the initial setup of a de novo genome sequencing...

Keywords: De novo

De Novo Assembly @ TGAC 2015 https://tess.elixir-europe.org/materials/de-novo-assembly-tgac-2015 This course took place between 12-15 May 2015 Overview: The course will provide an introduction to de novo assembly, with a hands on introduction followed by in-depth analysis of the key steps in the process. It covers several aspects such as the initial setup of a de novo genome sequencing project, quality control and preprocessing of datasets, generation and evaluation of first pass assemblies, assembly improvement and finishing. Practical exercises will be performed on small-scale real datasets, including Illumina and Pacbio sequences, including best practices and tips based on TGAC’s faculty experience. The course will consist of a mixture of conceptual lectures, methodological lectures and hands on sessions, as well as group activities and discussions. The participants will gain first-hand experience and understanding on NGS assembly, working with the assistance of the faculty, troubleshooting small problems, and reviewing the results. De novo PhD post-docs 2015-07-14 2017-10-09
Signalling Networks: From Data to Modelling

What is the course about? Signalling pathways and networks are very important to regulate and control cellular processes, cell fate, and their malfunctions often lead to diseases, such as cancer or diabetes. This course provides an introductory overview of the most useful resources and tools to...

Keywords: Signalling

Signalling Networks: From Data to Modelling https://tess.elixir-europe.org/materials/signalling-networks-from-data-to-modelling What is the course about? Signalling pathways and networks are very important to regulate and control cellular processes, cell fate, and their malfunctions often lead to diseases, such as cancer or diabetes. This course provides an introductory overview of the most useful resources and tools to investigate signalling pathways and networks, and also introduces basic approaches to model signalling events. The hands-on training will give a wide overview of tools and concepts related to signalling networks. What will I Learn? In this course you will learn how to use Cytoscape, where to find the most reliable signaling data, how to reconstruct, analyze and visualize a network. You will also learn how to develop your own models, mostly with logic modelling, what other modelling approaches exist, and how you can apply a model for your own research work. Signalling Grad students post-docs 2016-04-14 2017-10-09
A Critical Guide to BLAST

This Critical Guide in the Introduction to Bioinformatics series provides an overview of the BLAST similarity search tool, briefly examining the underlying algorithm and its rise to popularity. Several Web-based and stand-alone implementations are reviewed, and key features of typical search...

Keywords: Introduction bioinformatics, Introduction blast, Sequence database searching, Sequence similarity searching

A Critical Guide to BLAST https://tess.elixir-europe.org/materials/a-critical-guide-to-blast This Critical Guide in the Introduction to Bioinformatics series provides an overview of the BLAST similarity search tool, briefly examining the underlying algorithm and its rise to popularity. Several Web-based and stand-alone implementations are reviewed, and key features of typical search results are discussed. Specifically, this Guide introduces concepts and theories that underpin the BLAST search tool, and examines features of search outputs important for understanding and interpreting BLAST results. On reading this Guide, users will be able to: i) search a variety of Web-based sequence databases with different query sequences, and alter search parameters; ii) explain a range of typical search parameters, and the likely impacts on search outputs of changing them; iii) analyse the information conveyed in search outputs and infer the significance of reported matches; iv) examine and investigate the annotations of reported matches, and their provenance; and v) compare the outputs of different BLAST implementations and evaluate the implications of any differences. Introduction bioinformatics, Introduction blast, Sequence database searching, Sequence similarity searching Beginners 2018-09-08
A Critical Guide to InterPro

This Critical Guide in the Introduction to Bioinformatics series provides an introduction to the InterPro database, the largest, most comprehensive, integrated protein family database in the world. The rationale for creating the resource, the nature of its contributing databases and the kinds of...

Scientific topics: Database management

Keywords: Introduction bioinformatics, Introduction interpro, Protein family classification, Protein family databases, Protein family hierarchies, Protein function annotation, Protein sequence analysis

A Critical Guide to InterPro https://tess.elixir-europe.org/materials/a-critical-guide-to-interpro This Critical Guide in the Introduction to Bioinformatics series provides an introduction to the InterPro database, the largest, most comprehensive, integrated protein family database in the world. The rationale for creating the resource, the nature of its contributing databases and the kinds of information they provide are discussed, and the role of InterPro in protein classification and function-annotation projects is outlined. Specifically, this Guide introduces the principal components of the InterPro database, the differences between them, and how their integration creates a resource whose diagnostic power is greater than the sum of its parts. On reading this Guide, users will be able to: i) explain how protein family databases are used to help annotate uncharacterised protein sequences; ii) identify InterPro’s constituent data resources and explain the main methods that underpin them; iii) search InterPro using keywords and full sequences; iv) analyse and interpret search results in terms of protein family hierarchies, their structural domains and functional features; and v) track the provenance of InterPro’s annotations. Database management Introduction bioinformatics, Introduction interpro, Protein family classification, Protein family databases, Protein family hierarchies, Protein function annotation, Protein sequence analysis Beginners 2018-09-08
Summarising sets of phylogenies

A presentation given as part of the Basic Evolution Workshop, a trans-African virtual training course (described in this BioEssays article PMID: 21312200; the course wiki is here http://molecevol10.wikispaces.com/). Introduces concepts of splits, consensus trees, consensus networks, describes...

Keywords: Bootstrap, Consensus trees, Phylogenetic splits, Phylogenetics

Summarising sets of phylogenies https://tess.elixir-europe.org/materials/summarising-sets-of-phylogenies A presentation given as part of the Basic Evolution Workshop, a trans-African virtual training course (described in this BioEssays article PMID: 21312200; the course wiki is here http://molecevol10.wikispaces.com/). Introduces concepts of splits, consensus trees, consensus networks, describes examples of applications of these tools, along with introducing ideas of tree topology and some terminology associated with it. Designed for remote training. Bootstrap, Consensus trees, Phylogenetic splits, Phylogenetics Bench biologists 2013-10-23 2017-10-09
Flow Cytometry 2013 Module 4 - 1D Static gating

How to create a constant gate for the whole data set for one channel by first creating a sample representative of the whole data set How to apply a static gate to all samples and count the proportions of cells lying on either side of the gate Visualizing the proportions using a density...

Keywords: 1d static gating, Flow cytometry data

Flow Cytometry 2013 Module 4 - 1D Static gating https://tess.elixir-europe.org/materials/flow-cytometry-2013-module-4-1d-static-gating How to create a constant gate for the whole data set for one channel by first creating a sample representative of the whole data set How to apply a static gate to all samples and count the proportions of cells lying on either side of the gate Visualizing the proportions using a density plot, histogram, bean/violin plot Automating a sequential gating strategy in R to obtain cell proportions for a desired phenotype 1d static gating, Flow cytometry data 2013-06-26 2017-10-09
GBS

Training Materials from the 2015 GBS course run by TGAC

Keywords: Genotyping

GBS https://tess.elixir-europe.org/materials/gbs Training Materials from the 2015 GBS course run by TGAC Genotyping Graduate Students post-docs 2015-09-17 2017-10-09
GOBLET Poster at ISMB 2014

This is a PDF of the GOBLET poster presentation at ISMB 2014 in Boston.

Keywords: Goblet survey life scientists

GOBLET Poster at ISMB 2014 https://tess.elixir-europe.org/materials/goblet-poster-at-ismb-2014 This is a PDF of the GOBLET poster presentation at ISMB 2014 in Boston. Goblet survey life scientists 2014-07-07 2017-10-09
Python Programs

This is a module from the "Python for Biologists" course. It deals with Python programs, how to write and run them, and how to provide input and generate output. The module also contains exercises and suggested solutions. 

Keywords: Programming, Python, Python biologists

Python Programs https://tess.elixir-europe.org/materials/python-programs This is a module from the "Python for Biologists" course. It deals with Python programs, how to write and run them, and how to provide input and generate output. The module also contains exercises and suggested solutions.  Programming, Python, Python biologists Biologists Biologists, Genomicists, Computer Scientists beginner bioinformaticians 2013-11-04 2017-10-09
Pathway and Network Analysis 2014 Module 3 - Gene Regulation Analysis

Overview of transcription Data sources for regulatory data - Chromatin IP, DNA hypersensitivity, other chip data Basics of processing data Pattern discovery

Keywords: Pattern discovery, Transcriptional regulation

Pathway and Network Analysis 2014 Module 3 - Gene Regulation Analysis https://tess.elixir-europe.org/materials/pathway-and-network-analysis-2014-module-3-gene-regulation-analysis Overview of transcription Data sources for regulatory data - Chromatin IP, DNA hypersensitivity, other chip data Basics of processing data Pattern discovery Pattern discovery, Transcriptional regulation Biologists, Genomicists, Computer Scientists Graduate Students Post-Doctoral Fellows Researchers 2014-06-26 2017-10-09
RNA-seq Analysis 2014 Module 1 - Introduction to RNA-seq Analysis

Basic introduction to biology of RNA-seq Experimental design considerations Commonly asked questions Data visualization using IGV  

Keywords: Experimental design rna seq, Igv, Introduction rna seq analysis

RNA-seq Analysis 2014 Module 1 - Introduction to RNA-seq Analysis https://tess.elixir-europe.org/materials/rna-seq-analysis-2014-module-1-introduction-to-rna-seq-analysis Basic introduction to biology of RNA-seq Experimental design considerations Commonly asked questions Data visualization using IGV   Experimental design rna seq, Igv, Introduction rna seq analysis Biologists, Genomicists, Computer Scientists Graduate Students Post-Doctoral Fellows Researchers 2014-06-27 2017-10-09
Introduction to Linux Command Line

Introduction to Linux basic command line

Scientific topics: Computer science

Keywords: Linux basics

Introduction to Linux Command Line https://tess.elixir-europe.org/materials/introduction-to-linux-command-line Introduction to Linux basic command line Computer science Linux basics 2015-10-28 2017-10-09
Presentation About GOBLET Portal

This is a presentation I gave at the Jornadas de Bioinformática in Seville, Spain (24/09/14)

Keywords: Goblet, Training portal

Presentation About GOBLET Portal https://tess.elixir-europe.org/materials/presentation-about-goblet-portal This is a presentation I gave at the Jornadas de Bioinformática in Seville, Spain (24/09/14) Goblet, Training portal Bioinformatics General Interest Trainers Users 2014-09-26 2017-10-09
Train the Trainer course materials

Five PowerPoint presentations and an Excel worksheet for a Train the Trainer course developed by Chris Taylor (with contributions from Emily Angiolini, Jamie Edwards and Paul Yorke) while at the Earlham Institute working for the National Institutes of Science's Bioinformatics and Biomathematics...

Keywords: Course development, Pedagogy, Trainer skills

Train the Trainer course materials https://tess.elixir-europe.org/materials/train-the-trainer-course-materials Five PowerPoint presentations and an Excel worksheet for a Train the Trainer course developed by Chris Taylor (with contributions from Emily Angiolini, Jamie Edwards and Paul Yorke) while at the Earlham Institute working for the National Institutes of Science's Bioinformatics and Biomathematics Training hub. The five slide decks cover pedagogical theory, trainer skills and course development. The Excel workbook implements a version of Honey and Mumford's Learning Styles Questionnaire. Course development, Pedagogy, Trainer skills Prospective and existing trainers 2017-06-30 2017-10-09
Interactive bioinformatics 'taster' for students - exploring sickle cell anaemia

Developed in collaboration with the Royal Society, this material provides a short interactive introduction to bioinformatics, and includes a bioinformatics 'game' that simulates database searching and protein sequence analysis, with a focus on sickle cell anaemia. This is a fun activity with...

Interactive bioinformatics 'taster' for students - exploring sickle cell anaemia https://tess.elixir-europe.org/materials/interactive-bioinformatics-taster-for-students-exploring-sickle-cell-anaemia Developed in collaboration with the Royal Society, this material provides a short interactive introduction to bioinformatics, and includes a bioinformatics 'game' that simulates database searching and protein sequence analysis, with a focus on sickle cell anaemia. This is a fun activity with which to introduce bioinformatics to school students. 2018-03-15
Bioinformatics: Gene-protein-structure-function

This presentation examines the available in silico tools for protein structure and function prediction. It examines the major protein family databases (PROSITE, PRINTS, Pfam, etc.), and explores why tools like PSI-BLAST, while convenient and easy to use, may not always give optimal results. The...

Keywords: Expert systems, Genequiz, Protein family characterisation, Protein family databases, Protein sequence analysis, Psi blast

Bioinformatics: Gene-protein-structure-function https://tess.elixir-europe.org/materials/bioinformatics-gene-protein-structure-function This presentation examines the available in silico tools for protein structure and function prediction. It examines the major protein family databases (PROSITE, PRINTS, Pfam, etc.), and explores why tools like PSI-BLAST, while convenient and easy to use, may not always give optimal results. The presentation concludes with an analysis of 'expert' systems and integrated approaches (GeneQuiz, MAGPIE, InterPro, etc.), and dispels the commonly held notion that one database and/or one analysis tool is best. Expert systems, Genequiz, Protein family characterisation, Protein family databases, Protein sequence analysis, Psi blast beginner bioinformaticians biocurators postdoc postgrad 2013-07-12 2017-10-09
Writing functions in Python programming

This is a module from the "Python for Biologists" course. It deals with functions and how to write and use them. It also introduces namespaces and the tuple data structure. The module contains several exercises and suggested solutions. The text of exercises is also provided in a separate file. 

Scientific topics: Bioinformatics

Keywords: Programming, Python, Python biologists

Writing functions in Python programming https://tess.elixir-europe.org/materials/writing-functions-in-python-programming This is a module from the "Python for Biologists" course. It deals with functions and how to write and use them. It also introduces namespaces and the tuple data structure. The module contains several exercises and suggested solutions. The text of exercises is also provided in a separate file.  Bioinformatics Programming, Python, Python biologists Biologists Biologists, Genomicists, Computer Scientists beginner bioinformaticians 2013-11-04 2017-10-09
Multiple sequence alignment and phylogeny - undergraduate lab

This is lab 4 in a series of labs given as part of an undergraduate "Bioinformatics for biologists" course delivered to 2nd and 3rd year biology and bioinformatics undergraduate students, as part of a course focusing on using bioinformatics tools. Some instructions in it are specific to our lab...

Keywords: Clustalw, Multiple sequence alignment, Phylip, Phylogeny

Multiple sequence alignment and phylogeny - undergraduate lab https://tess.elixir-europe.org/materials/multiple-sequence-alignment-and-phylogeny-undergraduate-lab This is lab 4 in a series of labs given as part of an undergraduate "Bioinformatics for biologists" course delivered to 2nd and 3rd year biology and bioinformatics undergraduate students, as part of a course focusing on using bioinformatics tools. Some instructions in it are specific to our lab environment - including some not so current programs (clustalw, treetool, seaview, phylip) as that's what we have installed at the moment and they're stable, but the content can easily be adapted to other environments and programs. The lab is meant to be easily assessed and I get the students to answer the question in a moodle quiz which makes marking easier. The lab follows some lectures on multiple sequence alignment and molecular phylogeny. Clustalw, Multiple sequence alignment, Phylip, Phylogeny biology and bioinformatics sophomore undergraduates 2013-11-12 2017-10-09
Problem Based Learning Guide

Problem Based Learning (PBL) is an innovative approach to learning that we used to deliver the Clinical Bioinformatics I module of the new MSc in Clinical Bioinformatics. Clinical Bioinformatics is one of the streams of the NHS Scientific Training Programme (STP). The programme is a mixture of...

Keywords: Problem based learning, Teaching aid, Train trainer

Problem Based Learning Guide https://tess.elixir-europe.org/materials/problem-based-learning-guide Problem Based Learning (PBL) is an innovative approach to learning that we used to deliver the Clinical Bioinformatics I module of the new MSc in Clinical Bioinformatics. Clinical Bioinformatics is one of the streams of the NHS Scientific Training Programme (STP). The programme is a mixture of work placement based training interspersed with academic teaching which is delivered by Nowgen/NGRL and the University of Manchester in the UK.  This booklet is a guide for lecturers to design and deliver material using the PBL approach. It has been a successful tool for the delivery of our course and it may provide useful for others involved in course design and delivery Problem based learning, Teaching aid, Train trainer Lecturers Trainers 2014-01-12 2017-10-09
GOBLET Training portal

Presention

Keywords: Goblet

GOBLET Training portal https://tess.elixir-europe.org/materials/goblet-training-portal Presention Goblet 2015-07-01 2017-10-09
Phylogenetics of animal viral pathogens

Background The vast diversity of the pathogens affecting livestock demands a very specific diagnostic procedure in identification and characterization of each pathogen. In this context, the enormous amount of sequence and genotype data is being generated on animal pathogens, which is further...

Keywords: Animal pathogens, E learning, Phylogenetics

Phylogenetics of animal viral pathogens https://tess.elixir-europe.org/materials/phylogenetics-of-animal-viral-pathogens Background The vast diversity of the pathogens affecting livestock demands a very specific diagnostic procedure in identification and characterization of each pathogen. In this context, the enormous amount of sequence and genotype data is being generated on animal pathogens, which is further useful in understanding their pathogenicity and molecular epidemiology. The usage of this data in developing efficient molecular diagnostic tools needs basic understanding of the phylogenetic analysis. Phylogenetic classification, construction of trees, interpretation unveils the geographical distribution and migration of pathogens which helps in better management of animal diseases. The present course is designed with introduction to phylogenetics, tools, building and interpreting trees and finally its application to veterinary diagnostics. Though the course is driven in veterinary aspects, the same implicates to the human and plant pathogen study. Goal The ultimate aim is the better management of animal diseases by preparing laboratory technicians, veterinarians and molecular epidemiologists from diagnostic and research laboratories of developing FAO and IAEA member states, to be self-sufficient in the data analysis by interpreting the phylogenetic trees and their relationships. Target audience Researchers, Laboratory technicians, veterinarians, epidemiologists from diagnostic and research laboratories of member states of IAEA and FAO. The concepts are explained in generalized way to help all academicians, researchers, students from all countries working in the field of molecular genetics and epidemiology. AuthorsViral-Zone, Swiss-Prot Group at SIB Swiss Institute of Bioinformatics   Animal pathogens, E learning, Phylogenetics Bench biologists Biologists Laboratory technicians Researchers Veterinarians 2014-04-28 2017-10-09
Bioinformatics Introductory Module

This Bioinformatics Introductory Module for self-study was designed for future students of the  Molecular Mechanisms of Disease (MMD) master’s program at Radboud University Medical Centre. To level out the starting differences in bioinformatics knowledge between this incoming heterogeneous group...

Keywords: Bioinformatics, Genome browsing, Genomics, Protein structure

Bioinformatics Introductory Module https://tess.elixir-europe.org/materials/bioinformatics-introductory-module This Bioinformatics Introductory Module for self-study was designed for future students of the  Molecular Mechanisms of Disease (MMD) master’s program at Radboud University Medical Centre. To level out the starting differences in bioinformatics knowledge between this incoming heterogeneous group of students as much as possible, we have designed an introductory course module that should allow them – via self study and hands-on exercises – to acquire sufficient basic bioinformatics knowledge. Keywords: Databases, Sequence homology, Phylogeny, Protein structure, Microarrays, Proteomics, Expression analysis, Protein-protein interaction, Cluster analysis, protein modelling, networks, bioinformatics. Anticipated time needed to complete the module: 40 hours.  Answers for all the exercises are available on request. Of course you do need internet connection to perform the hands-on exercises. There is no need, however, to install programs onto your computer and also low-speed data transfer should be sufficient. All literature that is cited and used throughout the module was taken from open access sources.   Bioinformatics, Genome browsing, Genomics, Protein structure Life Science Researchers Master students 2014-06-27 2017-10-09
RNA-seq Analysis 2014 Module 2 - Alignment and Visualization

Use of Bowtie/TopHat ‘Regular mode’ vs. ‘Fusion mode’ Introduction to the BAM format Basic manipulation of BAMs with samtools, etc. Visualization of RNA-seq alignments - IGV BAM read counting and determination of variant allele expression status    

Keywords: Data visualization, Rna seq alignment

RNA-seq Analysis 2014 Module 2 - Alignment and Visualization https://tess.elixir-europe.org/materials/rna-seq-analysis-2014-module-2-alignment-and-visualization Use of Bowtie/TopHat ‘Regular mode’ vs. ‘Fusion mode’ Introduction to the BAM format Basic manipulation of BAMs with samtools, etc. Visualization of RNA-seq alignments - IGV BAM read counting and determination of variant allele expression status     Data visualization, Rna seq alignment Biologists, Genomicists, Computer Scientists Graduate Students Post-Doctoral Fellows Researchers 2014-06-27 2017-10-09
BioJS

BioJS is a library of graphical components easy to reuse to represent biological information

Keywords: Bioinformatics, Javascript

BioJS https://tess.elixir-europe.org/materials/biojs BioJS is a library of graphical components easy to reuse to represent biological information Bioinformatics, Javascript bioinformaticians web developers 2013-07-11 2017-10-09
Plant and Pathogen Bioinformatics

This package contains presentations and other training material given at the AllBio Plant and Pathogen Bioinformatics training course, held at the European Bioinformatics Institute, Hinxton, United Kingdom, Tuesday, July 8, 2014 - Friday, July 11, 2014. The course was aimed at giving an...

Keywords: Allbio, Bioinformatics, Biological databases, Genome sequence analysis, Pathogenesis, Phytopathogens, Plants

Plant and Pathogen Bioinformatics https://tess.elixir-europe.org/materials/plant-and-pathogen-bioinformatics This package contains presentations and other training material given at the AllBio Plant and Pathogen Bioinformatics training course, held at the European Bioinformatics Institute, Hinxton, United Kingdom, Tuesday, July 8, 2014 - Friday, July 11, 2014. The course was aimed at giving an introduction to researchers wishing to work in the plant-pathogen interactions area, covering major database resources and basis data analysis techniques. The aims of the course were to teach participants how to browse and access a range of data types from leading public repositories; use bioinformatic tools to explore plant and pathogen-related biological data; discuss the benefits and limitations of various bioinformatic techniques; and assess the purpose of specific analyses in a wider context. We would like to thank all presenters for their contributions and their willingness to further distribute their material via Goblet. Allbio, Bioinformatics, Biological databases, Genome sequence analysis, Pathogenesis, Phytopathogens, Plants beginner bioinformaticians early stage phytopathogen researchers 2014-09-11 2017-10-09
Metagenome data analysis Workshop, May 21-23, 2014

SciLifelab and AllBio are arranging a workshop on the analysis and interpretation of metagenomics data. In this workshop we will combine talks by experts in the field with practical tutorials guiding the participants in the analysis of representative datasets. Speakers: Lex Nederbragt, Oslo...

Keywords: Allbio, Assembly, Binning, Bioinformatics, Data analysis, Metagenomics

Metagenome data analysis Workshop, May 21-23, 2014 https://tess.elixir-europe.org/materials/metagenome-data-analysis-workshop-may-21-23-2014 SciLifelab and AllBio are arranging a workshop on the analysis and interpretation of metagenomics data. In this workshop we will combine talks by experts in the field with practical tutorials guiding the participants in the analysis of representative datasets. Speakers: Lex Nederbragt, Oslo University, Norway, Saskia Smits, Erasmus University Rotterdam, Netherlands, Joakim Larsson, Göteborg University, Sweden Paul Wilmes, University of Luxembourg, Luxembourg, Anders Andersson, SciLifeLab, Sweden Noan Le Bescot, UPMC (Tara expedition), France Tutorial supervisors: Ino de Bruijn, SciLifeLab, Sweden Johannes Alneberg, SciLifeLab, Sweden Luisa Hugerth, SciLifeLab, Sweden Johan Bengtsson, Göteborg University, Sweden Mikael Huss, SciLifeLab, Sweden Venue: Science for Life Laboratory, Tomtebodavägen 23A, 17165 Solna, Sweden Conference room Air/Fire, Gamma building Organisers: Mikael Huss (mikael.huss@scilifelab.se) Thomas Svensson (thomas.svensson@scilifelab.se) Allbio, Assembly, Binning, Bioinformatics, Data analysis, Metagenomics 2014-09-29 2017-10-09
A mind map is a nice visual way of describing the content of a training course

A Mind Map can be built by arranging boxes with subject titles in a variety of ways. In this example, the objective was to create a visual aid to better understand the relationship between the subjects that had been covered. The diagram is centered in the core subject, and other subjects occupy...

Keywords: Mind map, Training technique, Visual aid

A mind map is a nice visual way of describing the content of a training course https://tess.elixir-europe.org/materials/a-mind-map-is-a-nice-visual-way-of-describing-the-content-of-a-training-course A Mind Map can be built by arranging boxes with subject titles in a variety of ways. In this example, the objective was to create a visual aid to better understand the relationship between the subjects that had been covered. The diagram is centered in the core subject, and other subjects occupy successive layers showing hierarchy. This diagram was generated at the end of the GTPB training course SMLMC15 - Structural Modelling for Large Macromolecular Complexes with Joanna Kasprzak and Mateusz Dobrychłop It was prepared using the X-Mind software (free version) Mind map, Training technique, Visual aid Structural Biologists Training Designers Training instructors 2015-05-09 2017-10-09
TGAC_SeqAhead: NGS current challenges and data analysis for plant researchers

Hands on course held at TGAC (http://www.tgac.ac.uk/) on Monday 19 to Friday 23 May 2014 What is this workshop about? The course will combine lectures and led discussions to identify key challenges, opportunities and bottlenecks, with practical session on: Automated and standardized data...

Scientific topics: Sequence analysis, Plant biology, Database management, Bioinformatics

Keywords: Ngs, Ngs bioinformatics, Plants bioinformatics

TGAC_SeqAhead: NGS current challenges and data analysis for plant researchers https://tess.elixir-europe.org/materials/tgac_seqahead-ngs-current-challenges-and-data-analysis-for-plant-researchers Hands on course held at TGAC (http://www.tgac.ac.uk/) on Monday 19 to Friday 23 May 2014 What is this workshop about? The course will combine lectures and led discussions to identify key challenges, opportunities and bottlenecks, with practical session on: Automated and standardized data analysis for plant species data Data quality checks Estimation of reproducibility Batch effects Statistical concepts Importance of standards Data formats Data integration What will I learn? To discuss and reflect on best practice of experimental design and control for using NGS technologies in their context of addressing biological questions Identify key challenges, opportunities and bottlenecks Offering a platform for discussion and exploration of the challenges in plant research with a particular emphasis for agri-bioinformatics Sequence analysis Plant biology Database management Bioinformatics Ngs, Ngs bioinformatics, Plants bioinformatics PhD students plant researchers post-docs 2015-06-22 2017-10-09
Python @ TGAC - Python for Life Scientists: Managing biological data with Python

Python is an object-oriented programming language that is ideal for biological data analysis. The course will start with very basic language concepts and instructions and will cover all the main language aspects, including variables, types, modules, functions, exceptions, control of flux, input,...

Keywords: Biopython, Python, Python biologists

Python @ TGAC - Python for Life Scientists: Managing biological data with Python https://tess.elixir-europe.org/materials/python-tgac-python-for-life-scientists-managing-biological-data-with-python Python is an object-oriented programming language that is ideal for biological data analysis. The course will start with very basic language concepts and instructions and will cover all the main language aspects, including variables, types, modules, functions, exceptions, control of flux, input, output, and classes. All the examples and practical sessions will focus on solving particular biological problems. In particular, examples and practical sessions will cover: Working with DNA and protein sequences Data retrieval from files and their manipulation Running applications, such as BLAST, locally and from a script Finding motifs in sequences Parsing Swiss-Prot files, PDB files, ENSEMBL records, blast output files, etc. Biopython will be also introduced and applied to some of the mentioned examples. The course is meant to be highly interactive and the students will continuously put theory into practice while learning. By the end of the course, the students will have a good understanding of Python basics and will have acquired the skills to manage any type of bioinformatics record and to run applications from scripts. Unix/Linux basic skills will be provided at the beginning of the course. Biopython, Python, Python biologists PhD post-docs 2015-07-14 2017-10-09
RNA-seq data analysis: from raw reads to differentially expressed genes

This course material introduces the central concepts, analysis steps and file formats in RNA-seq data analysis. It covers the analysis from quality control to differential expression detection, and workflow construction and several data visualizations are also practised. The material consists of...

Scientific topics: Sequencing, RNA, Data architecture, analysis and design, Bioinformatics

Keywords: Bioinformatics, Differential expression, Ngs, Rna seq

RNA-seq data analysis: from raw reads to differentially expressed genes https://tess.elixir-europe.org/materials/rna-seq-data-analysis-from-raw-reads-to-differentially-expressed-genes This course material introduces the central concepts, analysis steps and file formats in RNA-seq data analysis. It covers the analysis from quality control to differential expression detection, and workflow construction and several data visualizations are also practised. The material consists of 10-30 minute lectures intertwined with hands-on exercises, and it can be accomplished in a day. As the user-friendly Chipster software is used in the exercises, no prior knowledge of R/Bioconductor or Unix ir required, and the course is thus suitable for everybody. Our book RNA-seq data analysis: A practical approach (CRC Press) can be used as background reading. The following topics and analysis tools are covered: 1. Introduction to the Chipster analysis platform 2. Quality control of raw reads (FastQC, PRINSEQ) 3. Preprocessing (Trimmomatic, PRINSEQ) 4. Alignment to reference genome (TopHat2) 5. Alignment level quality control (RseQC) 6. Quantitation (HTSeq) 7. Experiment level quality control with PCA and MDS plots 8. Differential expression analysis (DESeq2, edgeR) -normalization -dispersion estimation -statistical testing -controlling for batch effects, multifactor designs -filtering -multiple testing correction 9. Visualization of reads and results -genome browser -Venn diagram -volcano plot -plotting normalized counts for a gene -expression profiles 10. Experimental design Sequencing RNA Data architecture, analysis and design Bioinformatics Bioinformatics, Differential expression, Ngs, Rna seq Bench biologists Life Science Researchers 2015-12-04 2017-10-09
EMBER - a practical guide to bioinformatics

EMBER is an online practical designed to provide a brief, hands-on introduction to a range of bioinformatics databases and tools on the Web. It comprises basic and slightly more advanced 'chapters', and a number of case studies. Chapters include reflective questions, to stimulate critical...

EMBER - a practical guide to bioinformatics https://tess.elixir-europe.org/materials/ember-a-practical-guide-to-bioinformatics EMBER is an online practical designed to provide a brief, hands-on introduction to a range of bioinformatics databases and tools on the Web. It comprises basic and slightly more advanced 'chapters', and a number of case studies. Chapters include reflective questions, to stimulate critical thinking about results, and end-of-chapter quizzes. A glossary of terms and further reading is also provided. The basic chapters introduce students to practical protein sequence analysis, looking at protein sequence and protein family database searching, sequence alignment and 3D structure classification. For new users, the system requires creation of an account - in the registration form, select "independent user". Once an account has been created, log in to continue (make sure you have cookies enabled in your browser before proceeding). 2018-03-15
A Critical Guide to the PDB

This Critical Guide in the Introduction to Bioinformatics series provides a brief outline of the Protein Data Bank – the PDB – the world’s primary repository of biological macromolecular structures. The rationale for creating the resource and the kinds of information it provides are discussed,...

Scientific topics: Database management

Keywords: Introduction bioinformatics, Introduction pdb, Protein structure analysis, Protein structure databases, Protein structures

A Critical Guide to the PDB https://tess.elixir-europe.org/materials/a-critical-guide-to-the-pdb This Critical Guide in the Introduction to Bioinformatics series provides a brief outline of the Protein Data Bank – the PDB – the world’s primary repository of biological macromolecular structures. The rationale for creating the resource and the kinds of information it provides are discussed, and issues relating to its evolution and growth are explored. Specifically, this Guide introduces the principal features of the PDB, the nature (and quality) of its contents and how these may be interrogated. On reading this Guide, users will be able to: i) explain some of the ways in which knowledge of protein structures is useful; ii) identify the constituent databases of the wwPDB; iii) explain key features of the RCSB PDB in terms of its data distribution, growth and redundancy statistics; iv) search the PDB using simple and advanced keywords and full sequences, and analyse differences between them; and v) explain various structural quality criteria, and infer the quality of individual PDB entries. Database management Introduction bioinformatics, Introduction pdb, Protein structure analysis, Protein structure databases, Protein structures Beginners 2018-09-08
BD2K Data Science Open Educational Resources (OERs)

These Online Educational Resources (OERs) cover a variety of topics related to data science and are intended for use in courses, programs, workshops, and related activities. Modules relevant to biocuration:BDK05 Basic Research Data Standards...

Scientific topics: Ontology

Keywords: Biocuration, Curation, Identifiers, Ontology, Standards

BD2K Data Science Open Educational Resources (OERs) https://tess.elixir-europe.org/materials/bd2k-data-science-open-educational-resources-oers These Online Educational Resources (OERs) cover a variety of topics related to data science and are intended for use in courses, programs, workshops, and related activities. Modules relevant to biocuration:BDK05 Basic Research Data Standards (https://github.com/OHSUBD2K/BDK05-Basic-Research-Data-Standards). Standards for biomedical data, participating in communities that develop them, aiding reproducibility.BDK11 Identifiers (https://github.com/OHSUBD2K/BDK11-Identifiers). Introduction to identifiers (modeling, identifying and describing resources and objects.)BDK12 Data Annotation And Curation (https://github.com/OHSUBD2K/BDK12-Data-annotation-and-curation). Data preparation, developing standardized quality assurance processes and pipelines.BDK14 Ontologies 101 (https://github.com/OHSUBD2K/BDK14-Ontologies-101). Introduction to OWL2 and data reasoning.Authors: OHSU BD2K teamType of Material: Presentation slides, videos (in BDK12), exercises, and other resources.License: CC-BY Ontology Biocuration, Curation, Identifiers, Ontology, Standards biocurators 2018-04-02
QuickGuides

A range of Quick Guides to commonly used bioinformatics tools and resources.

Keywords: Blast, Emboss, Mysql, Perl, Phylip, Unixlinux, Velvet

QuickGuides https://tess.elixir-europe.org/materials/quickguides A range of Quick Guides to commonly used bioinformatics tools and resources. Blast, Emboss, Mysql, Perl, Phylip, Unixlinux, Velvet Beginners 2014-07-31 2017-10-09
Flow Cytometry 2013 Module 2 - Exploring FCM data in R

Loading a single or groups of FCS files into R flowFrame and flowSet objects and their attributes Exploring sample annotation and keywords stored within the FCS file format, searching for specic samples (such as controls) using grep, highlighting the importance of correct sample annotation...

Keywords: Fcs files, Plotting data, R

Flow Cytometry 2013 Module 2 - Exploring FCM data in R https://tess.elixir-europe.org/materials/flow-cytometry-2013-module-2-exploring-fcm-data-in-r Loading a single or groups of FCS files into R flowFrame and flowSet objects and their attributes Exploring sample annotation and keywords stored within the FCS file format, searching for specic samples (such as controls) using grep, highlighting the importance of correct sample annotation at point of acquisition Simple dot plots and density plots Fcs files, Plotting data, R 2013-06-26 2017-10-09
Bioinformatics for MS analysis

The purpose of this course is to teach students the fundamental concepts underlying the analysis of proteins using mass spectrometry.

Keywords: Bioinformatics, Identification, Mass spectrometry

Bioinformatics for MS analysis https://tess.elixir-europe.org/materials/bioinformatics-for-ms-analysis The purpose of this course is to teach students the fundamental concepts underlying the analysis of proteins using mass spectrometry. Bioinformatics, Identification, Mass spectrometry 2013-07-05 2017-10-09
Similarity searching, multiple sequence alignment and protein families - undergraduate lab

Lab 3 in a series of labs given as part of a "bioinformatics for biologists" course targeted at 2nd/3rd year undergraduates and focusing on informed use of tools. Some instructions are specific to our local computer lab setup but overall the content should be adaptable. Note that because some of...

Keywords: Blast, Hmmer, Interpro, Multiple sequence alignment, Similarity searching

Similarity searching, multiple sequence alignment and protein families - undergraduate lab https://tess.elixir-europe.org/materials/similarity-searching-multiple-sequence-alignment-and-protein-families-undergraduate-lab Lab 3 in a series of labs given as part of a "bioinformatics for biologists" course targeted at 2nd/3rd year undergraduates and focusing on informed use of tools. Some instructions are specific to our local computer lab setup but overall the content should be adaptable. Note that because some of the exercises make use of public databases they need to be checked and in some cases updated every time the lab is run. The lab is meant to be easily assessed using an online quiz (I use moodle). I provides a hands on introduction to NCBI BLAST, Interproscan, Clustal, MUSCLE and T-COFFEE, and HMMER.  Blast, Hmmer, Interpro, Multiple sequence alignment, Similarity searching biology and bioinformatics sophomore undergraduates 2013-11-12 2017-10-09
Clinical Bioinformatics I - Tutor notes

Clinical Bioinformatics I is a 10 credit module of the new MSc in Clinical Bioinformatics, delivered by Nowgen/NGRL and the University of Manchester in the UK. Clinical Bioinformatics is one of the streams of the NHS Scientific Training Programme (STP). The programme is a mixture of work...

Keywords: Clinical bioinformatics, Genomics, Introduction bioinformatics

Clinical Bioinformatics I - Tutor notes https://tess.elixir-europe.org/materials/clinical-bioinformatics-i-tutor-notes Clinical Bioinformatics I is a 10 credit module of the new MSc in Clinical Bioinformatics, delivered by Nowgen/NGRL and the University of Manchester in the UK. Clinical Bioinformatics is one of the streams of the NHS Scientific Training Programme (STP). The programme is a mixture of work placement based training interspersed with academic teaching.  The tutor notes describe the content taught on each day of the 5 day course, and the approach used to teach the materials. Each day is divided into two parts, a series of lectures in the morning to introduce the topics, then the afternoon sessions present a series of case studies for the students to work through in a 'problem based learning (PBL) approach to reinforce the lecture content.  Clinical bioinformatics, Genomics, Introduction bioinformatics Life Science Researchers healthcare professionals postgrad 2013-11-28 2017-10-09
Next Generation Sequencing (NGS) wikibook

Next generation sequencing (NGS) has become a commodity. With the commercialization of various affordable desktop sequencers, NGS will be of reach by more traditional wet-lab biologists . As seen in recent years, genome-wide scale computational analysis is increasingly being used as a backbone to...

Keywords: Bioinformatics, Next generation sequencing, Ngs

Next Generation Sequencing (NGS) wikibook https://tess.elixir-europe.org/materials/next-generation-sequencing-ngs-wikibook Next generation sequencing (NGS) has become a commodity. With the commercialization of various affordable desktop sequencers, NGS will be of reach by more traditional wet-lab biologists . As seen in recent years, genome-wide scale computational analysis is increasingly being used as a backbone to foster novel discovery in biomedical research. However, as the quantities of sequence data increase exponentially, the analysis bottle-neck is yet to be solved. The current sources for NGS informatics are extremely fragmented. A novice could read review articles in various journals, follow discussion threads on forums such as Biostar[1] or SEQanswers [2], or sign up for courses organized by various institutes. Finding a centralized synthesis is much more difficult. Books are available, but the development of the field is so fast that book chapters risk being obsoleted by the time they are even printed. Moreover, cost for a handful of authors to continually update their text would presumably take up a lot of their schedule. Drawing from the obvious goodwill and community spirit displayed on discussion forums, and exploiting the collaborative tools made available by the Wikimedia foundation, we propose to initiate the editing of a collaborative WikiBook on NGS. Our plan is to collect a sufficient amount of text that people will be incentivized to contribute to it, essentially providing the same information as a forum but in a tidier form. Ultimately, our goal is to create a collective lab book that explains the key concepts and describes best practices in NGS. Parnell LD, Lindenbaum P, Shameer K et al. BioStar: an online question & answer resource for the bioinformatics community, PLoS Comput Biol 2011;7:e1002216. Li JW, Schmieder R, Ward RM et al. SEQanswers: an open access community for collaboratively decoding genomes, Bioinformatics 2012;28:1272-1273 Join this initiative now and contribute to further expand and improve the contents and case studies in the NGS wikibook! Read about this initiative and the nine rules for NGS data analysis here: The NGS WikiBook: a dynamic collaborative online training effort with long-term sustainability Bioinformatics, Next generation sequencing, Ngs Biologists bioinformaticians computational scientists 2013-07-15 2017-10-09
BLAST and multiple sequence alignment (MSA) programs

Background The rapid identification of pathogens infecting livestock is essential to appropriately respond to the threat. The number and the variety of pathogen sequenced genomes have been growing more dramatically these recent years, because of the new sequencing technologies. This wealth of...

Keywords: Blast, E learning, Multiple sequence alignment

BLAST and multiple sequence alignment (MSA) programs https://tess.elixir-europe.org/materials/blast-and-multiple-sequence-alignment-msa-programs Background The rapid identification of pathogens infecting livestock is essential to appropriately respond to the threat. The number and the variety of pathogen sequenced genomes have been growing more dramatically these recent years, because of the new sequencing technologies. This wealth of new data is very useful to the research field through the development of bioinformatics tools and databases that deal with large amount of sequences. Among them, BLAST (Basic Local Alignment Search Tool) and MSA () programs are very efficient for protein or nucleotide sequence similarity search. The present course introduces the main biological databases, alignment tool BLAST and Multiple sequence alignments (MSA) programs, and interpretation of results. Goal The ultimate aim is the better management of animal diseases by preparing laboratory technicians, veterinarians and molecular epidemiologists from diagnostic and research laboratories of developing FAO and IAEA member states, to be self-sufficient in the data analysis by interpreting the phylogenetic trees and their relationships. Target audience Researchers, Laboratory technicians, veterinarians, epidemiologists from diagnostic and research laboratories of member states of IAEA and FAO. The concepts are explained in generalized way to help all academicians, researchers, students from all countries working in the field of molecular genetics and epidemiology. AuthorsViral-zone, Swiss-Prot group at the SIB Swiss Institute of Bioinformatics   Blast, E learning, Multiple sequence alignment Bench biologists Biologists Laboratory technicians Researchers Veterinarians 2014-04-28 2017-10-09
Pathway and Network Analysis 2014 Module 2 - Finding Over-represented Pathways

Over-representation analysis (ORA) Statistics for detecting over-representation e.g. hypergeometric test, GSEA Multiple testing correction: Bonferroni, Benjamini-Hochberg FDR Filtering Gene Ontology e.g. using evidence codes

Keywords: Gsea, Over representation analysis

Pathway and Network Analysis 2014 Module 2 - Finding Over-represented Pathways https://tess.elixir-europe.org/materials/pathway-and-network-analysis-2014-module-2-finding-over-represented-pathways Over-representation analysis (ORA) Statistics for detecting over-representation e.g. hypergeometric test, GSEA Multiple testing correction: Bonferroni, Benjamini-Hochberg FDR Filtering Gene Ontology e.g. using evidence codes Gsea, Over representation analysis Biologists, Genomicists, Computer Scientists Graduate Students Post-Doctoral Fellows Researchers 2014-06-26 2017-10-09
Basic bioinformatics and HOPE

Many bioinformatics tools have been developed over the last decennium, most of which were meant to be used in the lab of the life scientist. Unfortunately, only a few made it, because the tools are often too non-intuitive and too much a black box to the life scientist. Therefore Hanka Venselaar...

Keywords: Disease causing mutation, Predicting effect mutation protein function, Predicting effect mutation structure

Basic bioinformatics and HOPE https://tess.elixir-europe.org/materials/basic-bioinformatics-and-hope Many bioinformatics tools have been developed over the last decennium, most of which were meant to be used in the lab of the life scientist. Unfortunately, only a few made it, because the tools are often too non-intuitive and too much a black box to the life scientist. Therefore Hanka Venselaar developed HOPE to at least make the explanation of the phenotypic effect of mutations in (human) proteins intuitive for the non-bioinformatics expert. We developed this course to explain the basics of this program, and the underlying theory. Disease causing mutation, Predicting effect mutation protein function, Predicting effect mutation structure 2014-07-23 2017-10-09
Ensembl Browser Workshop: Plants and Microbes

Ensembl is a joint project between European Bioinformatics Institute (EMBL-EBI) and the Wellcome Trust Sanger Institute to provide a source of thorough, integrated annotation data across a variety of genomes. This one-day workshop not only offers participants the opportunity to glean background...

Ensembl Browser Workshop: Plants and Microbes https://tess.elixir-europe.org/materials/ensembl-browser-workshop-plants-and-microbes Ensembl is a joint project between European Bioinformatics Institute (EMBL-EBI) and the Wellcome Trust Sanger Institute to provide a source of thorough, integrated annotation data across a variety of genomes. This one-day workshop not only offers participants the opportunity to glean background information about the project and resulting resources, but also to gain vital hands-on experience in the use of the Ensembl genome browser. This workshop is themed according to the target audience and is aimed at wetlab researchers, as well as being customisable to include an introduction for complete novices up to advanced usage for the more frequent users. 2016-04-14 2017-10-09
A Critical Guide to the UniProtKB Flat-file Format

This Critical Guide briefly presents the need for biological databases and for a standard format for storing and organising biological data. Web-based interfaces have made databases more user-friendly, but knowledge of the underlying file format offers a deeper understanding of how to navigate...

Scientific topics: Database management

Keywords: Flat file databases, Flat files, Introduction bioinformatics, Uniprotkb flat file format

A Critical Guide to the UniProtKB Flat-file Format https://tess.elixir-europe.org/materials/a-critical-guide-to-the-uniprotkb-flat-file-format This Critical Guide briefly presents the need for biological databases and for a standard format for storing and organising biological data. Web-based interfaces have made databases more user-friendly, but knowledge of the underlying file format offers a deeper understanding of how to navigate and mine the information they contain, so that humans and machines can get the most out of them. This Guide explores the file format that underpins one of today’s most popular protein sequence databases – UniProtKB. Specifically, this Guide introduces the concept of database ‘flat-files’, and examines features of the UniProtKB flat-file format. On reading this Guide, users will be able to: i) identify key fields within UniProtKB/Swiss-Prot and UniProtKB/TrEMBL flat-files; ii) explain what these fields mean, what information they contain and what the information is used for; iii) analyse the information in different fields and infer structural and functional features of a sequence; iv) examine and investigate the provenance of annotations; and v) compare annotations at different time-points and evaluate the likely impact of annotation changes. Database management Flat file databases, Flat files, Introduction bioinformatics, Uniprotkb flat file format Beginners 2018-09-08
A Critical Guide to UniProtKB

This Critical Guide in the Introduction to Bioinformatics series provides a brief outline of the UniProt protein sequence database, with a particular focus on the UniProt Knowledgebase – UniProtKB. The rationale for creating the resource, its contributing databases and the kinds of information...

Scientific topics: Database management

Keywords: Introduction bioinformatics, Introduction uniprot, Protein sequence databases, Uniprot knowledgebase

A Critical Guide to UniProtKB https://tess.elixir-europe.org/materials/a-critical-guide-to-uniprotkb This Critical Guide in the Introduction to Bioinformatics series provides a brief outline of the UniProt protein sequence database, with a particular focus on the UniProt Knowledgebase – UniProtKB. The rationale for creating the resource, its contributing databases and the kinds of information they provide are discussed, and issues behind the quality of their annotations are explored. Specifically, this Guide introduces the principal components of the UniProt Knowledgebase, and the differences between them. On reading this Guide, users will be able to: i) identify and explain the characteristic features of UniProtKB/Swiss-Prot and UniProtKB/TrEMBL entries; ii) distinguish annotations that are computed, and hence not experimentally validated; iii) search UniProtKB using keywords, full sequences and peptides, and interpret the results; iv) analyse and track the provenance of annotations; and v) infer which annotations are likely to be accurate and which erroneous. Database management Introduction bioinformatics, Introduction uniprot, Protein sequence databases, Uniprot knowledgebase Beginners 2018-09-08
Flow Cytometry 2013 Module 5 - 1D Dynamic gating

Using quantiles to set a gate based on a negative control Using median and standard deviation to set a gate based on a negative control Exploring rangeGate: an automated way to set a 1D gate for each sample individually Recording proportions of cells in subpopulations of interest by...

Keywords: 1d dynamic gating, Flow cytometry data

Flow Cytometry 2013 Module 5 - 1D Dynamic gating https://tess.elixir-europe.org/materials/flow-cytometry-2013-module-5-1d-dynamic-gating Using quantiles to set a gate based on a negative control Using median and standard deviation to set a gate based on a negative control Exploring rangeGate: an automated way to set a 1D gate for each sample individually Recording proportions of cells in subpopulations of interest by creating a spreadsheet file Recording a visual record of the gating results by creating JPEG image files 1d dynamic gating, Flow cytometry data 2013-06-26 2017-10-09
Linear Motifs and Phosphorylation Sites

This is a lecture on linear motifs and phophorylation sites (P-sites). Some materials from other lectures are reused. The lecture is basically about computational approaches to encode, predict, analyse, and use functional motifs and P-sites. Here you can find: A definition of linear motifs and...

Keywords: Bioinformatics

Linear Motifs and Phosphorylation Sites https://tess.elixir-europe.org/materials/linear-motifs-and-phosphorylation-sites This is a lecture on linear motifs and phophorylation sites (P-sites). Some materials from other lectures are reused. The lecture is basically about computational approaches to encode, predict, analyse, and use functional motifs and P-sites. Here you can find: A definition of linear motifs and P-sites Computational methods to identify them How you can represent and use them Resources and tools to deal with them Bioinformatics Biologists biocurators bioinformaticians 2013-07-16 2017-10-09
Sequence comparison - undergraduate lab

This is lab 2 in a series of labs developed as part of a "bioinformatics for biology undergraduates" course and targeted at 2nd and 3rd year undergraduates. It is meant to be easily assessible. The lab includes some instructions that are specific to our computer lab environment but the content of...

Keywords: Dotmatrix plots, Scoring matrices, Sequence alignment

Sequence comparison - undergraduate lab https://tess.elixir-europe.org/materials/sequence-comparison-undergraduate-lab This is lab 2 in a series of labs developed as part of a "bioinformatics for biology undergraduates" course and targeted at 2nd and 3rd year undergraduates. It is meant to be easily assessible. The lab includes some instructions that are specific to our computer lab environment but the content of the lab should be easy to adapt to other environments. This lab makes use of EMBOSS programs, prss in the FASTA package, as well as the NCBI BLAST website. I usually deliver this lab after a series of lectures discussing dotmatrix plots and sequence alignment. Students enter their answers to the questions into a quiz set up within moodle which allows for easier marking. Dotmatrix plots, Scoring matrices, Sequence alignment Undergraduate students 2013-11-12 2017-10-09
Pathway and Network Analysis 2014 Module 1 - Introduction to Gene Lists

Gene list analysis overview: Workflow of concepts and tools from gene list to pathway analysis Where do gene lists come from? Working with gene function information

Keywords: Gene lists, Gene ontology

Pathway and Network Analysis 2014 Module 1 - Introduction to Gene Lists https://tess.elixir-europe.org/materials/pathway-and-network-analysis-2014-module-1-introduction-to-gene-lists Gene list analysis overview: Workflow of concepts and tools from gene list to pathway analysis Where do gene lists come from? Working with gene function information Gene lists, Gene ontology Biologists, Genomicists, Computer Scientists Graduate Students Post-Doctoral Fellows Researchers 2014-06-26 2017-10-09
Pathway and Network Analysis 2014 Module 4 - Pathway and Network Analysis

Introduction to pathway and network analysis Basic network concepts Types of pathway and network information Pathway Databases: Reactome, KEGG, etc. Pathway analysis of large scale genomics data sets, including cancer genomics

Keywords: Biological networks, Kegg, Pathway analysis, Reactome

Pathway and Network Analysis 2014 Module 4 - Pathway and Network Analysis https://tess.elixir-europe.org/materials/pathway-and-network-analysis-2014-module-4-pathway-and-network-analysis Introduction to pathway and network analysis Basic network concepts Types of pathway and network information Pathway Databases: Reactome, KEGG, etc. Pathway analysis of large scale genomics data sets, including cancer genomics Biological networks, Kegg, Pathway analysis, Reactome Biologists, Genomicists, Computer Scientists Graduate Students Post-Doctoral Fellows Researchers 2014-06-26 2017-10-09
InterPro: An introduction

This presentation introduces the background to the InterPro database: what it is, where it came from, and what was the vision behind its creation. It examines in particular whether the database has evolved in line with its original vision, and asks whether the resource is still fit for purpose. 

Keywords: Integrated diagnostic tools, Protein family characterisation, Protein sequence analysis

InterPro: An introduction https://tess.elixir-europe.org/materials/interpro-an-introduction This presentation introduces the background to the InterPro database: what it is, where it came from, and what was the vision behind its creation. It examines in particular whether the database has evolved in line with its original vision, and asks whether the resource is still fit for purpose.  Integrated diagnostic tools, Protein family characterisation, Protein sequence analysis beginner bioinformaticians biocurators postdoc postgrad 2013-07-12 2017-10-09
An Introduction to Unix, Perl and Python

This  course provides an introduction to Unix, Perl and Python. In addition to lecture material (in the form of slides), there are exercises, answers to the exercises and pointers to addition resources.

Keywords: Perl, Python, Unix

An Introduction to Unix, Perl and Python https://tess.elixir-europe.org/materials/an-introduction-to-unix-perl-and-python This  course provides an introduction to Unix, Perl and Python. In addition to lecture material (in the form of slides), there are exercises, answers to the exercises and pointers to addition resources. Perl, Python, Unix Bench biologists 2013-07-28 2017-10-09
Network Analysis with Cytoscape and PSICQUIC

This workshop will guide novice users through the process of analysing interaction networks – that allow biologists to map and characterise signalling pathways and to predict the function of unknown proteins. It will use practical examples in the popular open-source tool Cytoscape and the...

Keywords: Cytoscape

Network Analysis with Cytoscape and PSICQUIC https://tess.elixir-europe.org/materials/network-analysis-with-cytoscape-and-psicquic This workshop will guide novice users through the process of analysing interaction networks – that allow biologists to map and characterise signalling pathways and to predict the function of unknown proteins. It will use practical examples in the popular open-source tool Cytoscape and the PSICQUIC client to access several protein interaction repositories at the same time to integrate protein data from different sources. Data from external sources will then be incorporated using different Cytoscape apps to perform clustering and GO enrichment analysis over our newly created networks. Cytoscape Biologists, Genomicists, Computer Scientists 2016-04-14 2017-10-09
R as a programming language

This course is aimed at colleagues who would like to learn how to use the statistical programming language "R". We will learn about the data structures and functions in R, and how to write R scripts. Topics: Introduction to R: general principles. Data structures in R: vectors, matrices, arrays,...

Keywords: R programming

R as a programming language https://tess.elixir-europe.org/materials/r-as-a-programming-language This course is aimed at colleagues who would like to learn how to use the statistical programming language "R". We will learn about the data structures and functions in R, and how to write R scripts. Topics: Introduction to R: general principles. Data structures in R: vectors, matrices, arrays, lists, data frames. Data import/export. Functions in R: how to write your own functions and scripts. Visualization in R: various plot types (built-in graphics only). R programming 2016-04-21 2017-10-09
PRINTS: A protein family database with a difference

A presentation designed to introduce the concept of protein family analysis and characterisation using motif-based methods, with a particular focus on protein fingerprinting. Following a general introduction to sequence analysis, and the fingerprint approach, specific examples are given to...

Keywords: Functional diagnosis, Protein family characterisation, Protein sequence analysis

PRINTS: A protein family database with a difference https://tess.elixir-europe.org/materials/prints-a-protein-family-database-with-a-difference A presentation designed to introduce the concept of protein family analysis and characterisation using motif-based methods, with a particular focus on protein fingerprinting. Following a general introduction to sequence analysis, and the fingerprint approach, specific examples are given to demonstrate the importance of understanding how to interpret the results of different sequence analysis methods and, crucially, the differences between them, and the added value that fingerprints bring. Functional diagnosis, Protein family characterisation, Protein sequence analysis beginner bioinformaticians biocurators postdoc postgrad 2013-07-12 2017-10-09
Introduction to Multiple Sequence Alignment

An introduction to multiple sequence alignments (MSAs) for bench biologists delivered as part of the EMBL Australia Masterclass on Protein Sequence Analysis http://oz-masterclass.wikispaces.com/ . Focuses on describing: the "anatomy" of a sequence alignment; two alternative interpretations of...

Keywords: Multiple sequence analysis, Protein structure, Sequence analysis

Introduction to Multiple Sequence Alignment https://tess.elixir-europe.org/materials/introduction-to-multiple-sequence-alignment An introduction to multiple sequence alignments (MSAs) for bench biologists delivered as part of the EMBL Australia Masterclass on Protein Sequence Analysis http://oz-masterclass.wikispaces.com/ . Focuses on describing: the "anatomy" of a sequence alignment; two alternative interpretations of alignmetns (structural and evolutionary), and ways of building manual and automatic alignments, and an introduction to JalView Multiple sequence analysis, Protein structure, Sequence analysis Bench biologists 2013-10-23 2017-10-09
Flow Cytometry 2013 Module 3 - Preprocessing and Quality Assurance of FCM Data

Preprocessing Removing margin events Data transformation: log vs. biexponential Data normalization Quality Assurance Overview of quality assurance concepts: total raw/viable cell count, margin event count, outlier detection based on density of common...

Keywords: Flow cytometry data, Preprocessing, Quality assurance

Flow Cytometry 2013 Module 3 - Preprocessing and Quality Assurance of FCM Data https://tess.elixir-europe.org/materials/flow-cytometry-2013-module-3-preprocessing-and-quality-assurance-of-fcm-data Preprocessing Removing margin events Data transformation: log vs. biexponential Data normalization Quality Assurance Overview of quality assurance concepts: total raw/viable cell count, margin event count, outlier detection based on density of common parameters Building quality assurance objects using flowQ and generating summary HTML reports Flow cytometry data, Preprocessing, Quality assurance 2013-06-26 2017-10-09
Pattern Matching

This is a module from the "Python for Biologists" course. It teaches how to do pattern matching in Python, i.e. how to find a substring (or a set of substrings) in a string. To this aim, it introduces the regular expression syntax, and the tools needed to search regular expressions in biological...

Keywords: Pattern matching, Programming, Python, Python biologists

Pattern Matching https://tess.elixir-europe.org/materials/pattern-matching This is a module from the "Python for Biologists" course. It teaches how to do pattern matching in Python, i.e. how to find a substring (or a set of substrings) in a string. To this aim, it introduces the regular expression syntax, and the tools needed to search regular expressions in biological sequences and in regular text, such as PubMed abstracts. Exercises and suggested solutions are presented in a separate file. Pattern matching, Programming, Python, Python biologists Biologists Biologists, Genomicists, Computer Scientists beginner bioinformaticians 2013-11-04 2017-10-09
GGPLOT2 : Pragmatic GGPLOT

A fully contained course for GGPLOT2

GGPLOT2 : Pragmatic GGPLOT https://tess.elixir-europe.org/materials/ggplot2-pragmatic-ggplot A fully contained course for GGPLOT2 2017-06-12 2017-10-09
Searching data using Python

This is a module from the "Python for Biologists" course. It describes how to use Python dictionary and set data structures to search your data. In particular, how to use a dictionary to represent the genetic code table and use it to translate a nucleotide sequence into a protein sequence, and...

Keywords: Programming, Python, Python biologists

Searching data using Python https://tess.elixir-europe.org/materials/searching-data-using-python This is a module from the "Python for Biologists" course. It describes how to use Python dictionary and set data structures to search your data. In particular, how to use a dictionary to represent the genetic code table and use it to translate a nucleotide sequence into a protein sequence, and how to use sets to find unique records in two datasets and remove redundancy.  Programming, Python, Python biologists Biologists Biologists, Genomicists, Computer Scientists beginner bioinformaticians 2013-11-04 2017-10-09
Using R with Python

This is a module from the "Python for Biologists" course. It describes the Python module interfacing the R package for statistics. The module shows how to calculate mean, standard deviation, z-score and p-value of a set of numbers, and how to generate plots. Input files for the scripts presented...

Keywords: Programming, Python, Python biologists

Using R with Python https://tess.elixir-europe.org/materials/using-r-with-python This is a module from the "Python for Biologists" course. It describes the Python module interfacing the R package for statistics. The module shows how to calculate mean, standard deviation, z-score and p-value of a set of numbers, and how to generate plots. Input files for the scripts presented are also provided. Programming, Python, Python biologists Biologists Biologists, Genomicists, Computer Scientists beginner bioinformaticians bioinformaticians 2013-11-04 2017-10-09
Introduction to Biopython

This is a module from the "Python for Biologists" course. The module presents an introduction to Biopython. It shows how to deal with sequences and sequence records, how to download records from NCBI databases, how to run Blast and how to parse XML Blast outputs.

Keywords: Bioinformatics, Biopython, Programming, Python, Python biologists

Introduction to Biopython https://tess.elixir-europe.org/materials/introduction-to-biopython This is a module from the "Python for Biologists" course. The module presents an introduction to Biopython. It shows how to deal with sequences and sequence records, how to download records from NCBI databases, how to run Blast and how to parse XML Blast outputs. Bioinformatics, Biopython, Programming, Python, Python biologists Biologists Biologists, Genomicists, Computer Scientists bioinformaticians 2013-11-04 2017-10-09
EMBL-EBI Train Online

If you work in the life sciences, you may find that you’re spending less time doing experiments and more time analysing huge amounts of biological data. Train online is here to help you do this quickly and efficiently.

Scientific topics: Bioinformatics

Keywords: Bioinformatics, Chemical biology, Cross domain, Dna rna, Gene expression, Literature, Ontologies, Proteins, Structures, Systems

EMBL-EBI Train Online https://tess.elixir-europe.org/materials/embl-ebi-train-online If you work in the life sciences, you may find that you’re spending less time doing experiments and more time analysing huge amounts of biological data. Train online is here to help you do this quickly and efficiently. Bioinformatics Bioinformatics, Chemical biology, Cross domain, Dna rna, Gene expression, Literature, Ontologies, Proteins, Structures, Systems Beginner informatics Life sciences 2018-05-11
Parsing data records using Python programming

This is a module from the "Python for Biologists" course. One typical problem in bioinformatics is parsing data files. This module explains how to parse FASTA files and GenBank records. It also introduces the if/elif/else construct to make choice in programming and the list  data structure. The...

Keywords: Bioinformatics, Programming, Python, Python biologists, Record parsing

Parsing data records using Python programming https://tess.elixir-europe.org/materials/parsing-data-records-using-python-programming This is a module from the "Python for Biologists" course. One typical problem in bioinformatics is parsing data files. This module explains how to parse FASTA files and GenBank records. It also introduces the if/elif/else construct to make choice in programming and the list  data structure. The text of exercises is provided in a separate file.  Bioinformatics, Programming, Python, Python biologists, Record parsing Biologists beginner bioinformaticians bioinformaticians programmers 2013-07-05 2017-10-09
Pathway and Network Analysis 2014 Module 6 - Gene Function Prediction

Functional association networks and gene function prediction Homology based prediction Guilt-by-association: GeneMANIA

Keywords: Functional association networks, Gene function prediction, Genemania

Pathway and Network Analysis 2014 Module 6 - Gene Function Prediction https://tess.elixir-europe.org/materials/pathway-and-network-analysis-2014-module-6-gene-function-prediction Functional association networks and gene function prediction Homology based prediction Guilt-by-association: GeneMANIA Functional association networks, Gene function prediction, Genemania Biologists, Genomicists, Computer Scientists Graduate Students Post-Doctoral Fellows Researchers 2014-06-26 2017-10-09
High-throughput sequencing training materials repository

This repository includes training materials on the analysis of high-throughput sequencing (HTS) data, on the following topics: Introduction to HTS, RNA-seq, ChIP-seq and variant calling analysis. Materials have been annotated following the standards and guidelines proposed at the “Best practices...

Scientific topics: Data architecture, analysis and design, Bioinformatics

Keywords: High throughput sequencing analysis, Rna seq chip seq anayses, Variant calling

High-throughput sequencing training materials repository https://tess.elixir-europe.org/materials/high-throughput-sequencing-training-materials-repository This repository includes training materials on the analysis of high-throughput sequencing (HTS) data, on the following topics: Introduction to HTS, RNA-seq, ChIP-seq and variant calling analysis. Materials have been annotated following the standards and guidelines proposed at the “Best practices in next-generation sequencing data analysis” workshop which took place at the University of Cambridge, UK, on 13-14 January 2015. Following this workshop, a Git repository has been set up for sharing annotated materials. This repository uses Git, hence it is decentralized and self-managed by the community and can be forked/built-upon by all users. Data architecture, analysis and design Bioinformatics High throughput sequencing analysis, Rna seq chip seq anayses, Variant calling Life Science Researchers PhD students Trainers beginner bioinformaticians post-docs 2015-12-17 2017-10-09
RNA-seq analysis

I give an overview of the analysis of gene expression in plants using RNA-seq data. This course provides a foundation on the tuxedo software suite: TopHat, Cufflinks, CummeRbund.

RNA-seq analysis https://tess.elixir-europe.org/materials/rna-seq-analysis I give an overview of the analysis of gene expression in plants using RNA-seq data. This course provides a foundation on the tuxedo software suite: TopHat, Cufflinks, CummeRbund. 2013-06-28 2017-10-09
UNIX command line and scripting

The first part of this course introduces the UNIX command line on a Linux or Mac. The second part provides a basic introduction to string searches and scripting with AWK and BASH.

Keywords: Awk, Bash, Unix

UNIX command line and scripting https://tess.elixir-europe.org/materials/unix-command-line-and-scripting The first part of this course introduces the UNIX command line on a Linux or Mac. The second part provides a basic introduction to string searches and scripting with AWK and BASH. Awk, Bash, Unix 2016-04-21 2017-10-09
Simple plotting in R

This quick and simple tutorial demonstrates some of the easy plotting tools in the R core software. the data are in the "data" directory and come from an old two-colour microarray experiment where spots on the array were printed with different buffers, in different concentrations and with...

Keywords: Bioinformatics, Microarray data analysis, Plotting data, R, Visualisation

Simple plotting in R https://tess.elixir-europe.org/materials/simple-plotting-in-r This quick and simple tutorial demonstrates some of the easy plotting tools in the R core software. the data are in the "data" directory and come from an old two-colour microarray experiment where spots on the array were printed with different buffers, in different concentrations and with different pins.  Also, both channels Cy5 and Cy3 were spotted identically, and so the fold ratio for all spots should be 1 - this helps us see bias in the data Bioinformatics, Microarray data analysis, Plotting data, R, Visualisation Beginners PhD students Researchers Scientists 2014-01-14 2017-10-09
Working with Affymetrix CEL files in R

This tutorial shows how to download some public Affymetrix microarray data, load the data into R, calculate expression values and do some very simple plotting

Keywords: Affymetrix, Microarrays, R

Working with Affymetrix CEL files in R https://tess.elixir-europe.org/materials/working-with-affymetrix-cel-files-in-r This tutorial shows how to download some public Affymetrix microarray data, load the data into R, calculate expression values and do some very simple plotting Affymetrix, Microarrays, R Beginners PhD students Researchers 2014-01-14 2017-10-09
Pathway and Network Analysis 2014 Module 5 - Network Visualization

Introduction to network visualization Visualizing omics data on a network or pathway Active modules in Cytoscape

Keywords: Cytoscape, Network visualization

Pathway and Network Analysis 2014 Module 5 - Network Visualization https://tess.elixir-europe.org/materials/pathway-and-network-analysis-2014-module-5-network-visualization Introduction to network visualization Visualizing omics data on a network or pathway Active modules in Cytoscape Cytoscape, Network visualization Biologists, Genomicists, Computer Scientists Graduate Students Post-Doctoral Fellows Researchers 2014-06-26 2017-10-09
Perl 1 Training Course - For Programming Beginners

A set of slides from The Sainsbury Laboratory Perl course for absolute beginners

Keywords: Perl, Programming

Perl 1 Training Course - For Programming Beginners https://tess.elixir-europe.org/materials/perl-1-training-course-for-programming-beginners A set of slides from The Sainsbury Laboratory Perl course for absolute beginners Perl, Programming Beginners Bench biologists 2014-07-03 2017-10-09
Metagenomics 2015

Link to the GitHub repo containing all the publicly available files from the 2015 Metagenomics course held at TGAC in November 2015

Scientific topics: Metagenomics

Keywords: Metagenomics

Metagenomics 2015 https://tess.elixir-europe.org/materials/metagenomics-2015 Link to the GitHub repo containing all the publicly available files from the 2015 Metagenomics course held at TGAC in November 2015 Metagenomics Metagenomics PhD post-docs 2015-11-23 2017-10-09
TGAC Metagenomics: From bench to data analysis

This course took place at TGAC on 8-12 September 2014 What is the workshop about? A substantial part of the course will be devoted to bioinformatics resources and tools relevant in metagenomics data analysis. Participants will start at the bench and end in front of the computers for a problem...

Scientific topics: Metagenomics

Keywords: Metagenomics

TGAC Metagenomics: From bench to data analysis https://tess.elixir-europe.org/materials/tgac-metagenomics-from-bench-to-data-analysis This course took place at TGAC on 8-12 September 2014 What is the workshop about? A substantial part of the course will be devoted to bioinformatics resources and tools relevant in metagenomics data analysis. Participants will start at the bench and end in front of the computers for a problem solving activity utilizing the bioinformatics training they will be receiving during the course. What will it cover? To provide an overview of the main aspects involved in metagenomics from the bench to data analysis and discussion around the interpretation and actual examples of the impact and applications of metagenomics derived research Target Audience: This course is aimed at advanced PhD students and post-doctoral researchers who are doing or planning to start research in the field of metagenomics. Metagenomics Metagenomics PhD postdocs 2015-06-25 2017-10-09
De Novo

Training Materials from the 2015 De Novo course run by TGAC

Keywords: De novo, De novo genome assembly

De Novo https://tess.elixir-europe.org/materials/de-novo Training Materials from the 2015 De Novo course run by TGAC De novo, De novo genome assembly PhD PhD students post-docs postdoctoral researchers 2015-09-17 2017-10-09
EMBO 2015

Link to the GitHub repository containing all the training materials made available for the 2015 EMBO event held at TGAC

Scientific topics: Protein binding sites, Molecular interactions, pathways and networks

Keywords: Bioinformatics, Multiple sequence alignment, Network visualization, Protein protein interaction networks

EMBO 2015 https://tess.elixir-europe.org/materials/embo-2015 Link to the GitHub repository containing all the training materials made available for the 2015 EMBO event held at TGAC Protein binding sites Molecular interactions, pathways and networks Bioinformatics, Multiple sequence alignment, Network visualization, Protein protein interaction networks PhD students post-docs 2015-10-08 2017-10-09
Python programming primer

The purpose of this training is to teach general programming concepts using Python as an instruction tool. Topics: Introduction to Python: basic principles. Python data structures: strings, tuples, lists, dictionaries, sets. Object-oriented programming: how to model coffee machines in Python...

Scientific topics: Software engineering

Keywords: Python biologists

Python programming primer https://tess.elixir-europe.org/materials/python-programming-primer The purpose of this training is to teach general programming concepts using Python as an instruction tool. Topics: Introduction to Python: basic principles. Python data structures: strings, tuples, lists, dictionaries, sets. Object-oriented programming: how to model coffee machines in Python :-). Inheritance (base and derived classes), polymorphism. Write your own script to convert BED files to GFF. Command-line option processing, file I/O, error handling. Software engineering Python biologists 2016-04-21 2017-10-09
Statistics with R

The aim of this course is to teach you how to perform basic statistical analysis using R. First we review the foundations (sampling theory, discrete and continuous distributions), then we focus on classical hypothesis testing. This course will improve your generic statistics knowledge....

Scientific topics: Statistics and probability

Keywords: Biostatistics, R

Statistics with R https://tess.elixir-europe.org/materials/statistics-with-r The aim of this course is to teach you how to perform basic statistical analysis using R. First we review the foundations (sampling theory, discrete and continuous distributions), then we focus on classical hypothesis testing. This course will improve your generic statistics knowledge. Topics: Sampling theory: obtaining information about a population via sampling. Sample characteristics (location, dispersion, skewness), estimation of the mean, standard error of the mean. Discrete and continuous probability distributions. Central limit theorem. Hypothesis testing. Basic principles, one- and two-sided testing, types of errors, power calculations. "Cookbook of tests": location testing, normality, variance comparisons, counting statistics, contingency tables, regression tests. Statistics and probability Biostatistics, R 2016-04-21 2017-10-09
Intended Learning Outcome Advisor

This tool allows trainers to evaluate Intended Learning Outcomes (ILOs) they've written to accompany their training resources. The tool is able to assess sets of ILOs against representative verbs for each level of Bloom's Taxonomy, verifying both the Bloom's level to which they correspond and the...

Intended Learning Outcome Advisor https://tess.elixir-europe.org/materials/intended-learning-outcome-advisor This tool allows trainers to evaluate Intended Learning Outcomes (ILOs) they've written to accompany their training resources. The tool is able to assess sets of ILOs against representative verbs for each level of Bloom's Taxonomy, verifying both the Bloom's level to which they correspond and the appropriateness of the verbs used; where verbs appear ambiguous, the tool offers guidance to help improve the description of the respective ILO. 2018-02-15
Biocuration: An Introduction

Claire O'Donovan, leader of the Protein Function Content team at EMBL-EBI, gives an introduction into biocuration and talks about what it is like to work as a biocurator and the skill sets you need. This webinar was recorded on 2 March 2016 and is best viewed using Google Chrome and in...

Keywords: Biocuration

Biocuration: An Introduction https://tess.elixir-europe.org/materials/biocuration-an-introduction-bdbaa48f-b505-4b5d-affb-83328bc2fac1 Claire O'Donovan, leader of the Protein Function Content team at EMBL-EBI, gives an introduction into biocuration and talks about what it is like to work as a biocurator and the skill sets you need. This webinar was recorded on 2 March 2016 and is best viewed using Google Chrome and in full-screen mode. Biocuration Biologists biocurators life scientists 2018-05-11
A Critical Guide to Unix

This Critical Guide in the Introduction to Bioinformatics series briefly introduces the Unix Operating System, and provides a subset of some of the most helpful and commonly used commands, including those that allow various types of search, navigation and file manipulation. Several keystroke...

Keywords: Command line, Introduction bioinformatics, Introduction unix, Unix commands, Unix operating system

A Critical Guide to Unix https://tess.elixir-europe.org/materials/a-critical-guide-to-unix This Critical Guide in the Introduction to Bioinformatics series briefly introduces the Unix Operating System, and provides a subset of some of the most helpful and commonly used commands, including those that allow various types of search, navigation and file manipulation. Several keystroke short-cuts are also explained, which help to make the routine use of Unix commands more efficient. Specifically, this Guide showcases some of the simplest, most frequently used commands to help new users to understand and gain confidence in using the Unix Operating System. On reading the Guide, users will be able: i) to exploit a range of commands: to manipulate files, directories and processes; to navigate directory structures and explore their contents; to search for files, and search and compare file contents; and to direct command outputs into files or into other commands; and ii) to explain what many simple commands mean and how they’re used. Command line, Introduction bioinformatics, Introduction unix, Unix commands, Unix operating system Beginners 2018-09-08
Concepts, historical milestones & the central place of bioinformatics in modern biology: a European perspective

Short history of the emergence of bioinformatics as a discipline in Europe, following the near simultaneous advent of high-throughput sequencing techniques and the World Wide Web, which made dissemination of the rapidly accumulating sequence data both simple and feasible on a large scale.

Keywords: Bioinformatics history, Biomolecular databases, Biomolecular sequences, European perspective

Concepts, historical milestones & the central place of bioinformatics in modern biology: a European perspective https://tess.elixir-europe.org/materials/concepts-historical-milestones-the-central-place-of-bioinformatics-in-modern-biology-a-european-perspective Short history of the emergence of bioinformatics as a discipline in Europe, following the near simultaneous advent of high-throughput sequencing techniques and the World Wide Web, which made dissemination of the rapidly accumulating sequence data both simple and feasible on a large scale. Bioinformatics history, Biomolecular databases, Biomolecular sequences, European perspective 2013-06-25 2017-10-09
Flow Cytometry 2013 Module 6 - Clustering and Additional FCM Tools

Lecture on FlowCAP (Flow Cytometry: Critical Assessment of Population Identification Methods) project K-means explained flowMeans: smart k-means for FCM data flowClust3.0, SPADE, flowBin, flowFP, SamSPECTRAL clustering Biomarker discovery: flowType and RchyOptimyx, advanced tools used...

Keywords: Biomarker discovery, Flow cytometry data, Flowcap

Flow Cytometry 2013 Module 6 - Clustering and Additional FCM Tools https://tess.elixir-europe.org/materials/flow-cytometry-2013-module-6-clustering-and-additional-fcm-tools Lecture on FlowCAP (Flow Cytometry: Critical Assessment of Population Identification Methods) project K-means explained flowMeans: smart k-means for FCM data flowClust3.0, SPADE, flowBin, flowFP, SamSPECTRAL clustering Biomarker discovery: flowType and RchyOptimyx, advanced tools used to explore novel phenotypes and find ones which correlate with a clinical diagnosis More R: Bioconductor.org, an open source software project full of bioinformatics packages created for R, including sample work flows GenePattern.org: web-interface for running analysis modules such as sample deidentication, extracting specic keywords, quality control, normalization, clustering, classification FlowRepository.org: online FCM data repository to share public or private data sets with collaborators while providing detailed descriptions of the experiment set up and each FCS file Biomarker discovery, Flow cytometry data, Flowcap 2013-06-26 2017-10-09
UNIX Fundamentals

This self-learning tutorial aims to present the UNIX environment and to provide the most basic commands to users with no or very little UNIX knowledge. The examples are taken from various Biological fields but have been chosen carefully to be easily accessible to a wide audience. At the end of...

Keywords: Problem based learning, Programming, Unix

UNIX Fundamentals https://tess.elixir-europe.org/materials/unix-fundamentals-35adf82d-ae32-449d-9627-43b5d18da8cc This self-learning tutorial aims to present the UNIX environment and to provide the most basic commands to users with no or very little UNIX knowledge. The examples are taken from various Biological fields but have been chosen carefully to be easily accessible to a wide audience. At the end of the course, participants are expected to be able to: Have some understanding on UNIX systems Navigate through the UNIX filesystem Execute and understand some UNIX process commands Correctly set file permissions Perform basic file management Participants are encouraged to follow the tutorial sections sequentially. The tutorial content is sometimes an oversimplification of the Truth - yes, we know! Due to the diversity of computer systems, we will just assume that participants do have access to a terminal. For help, please contact your IT support - or a good friend!Authors are affiliated to the SIB Swiss Institute of Bioinformatics: Vassilios IoannidisWith some content provided by Frédéric Schütz, Volker Flegel and Heinz StockingerContent integration by Grégoire Rossier and Vassilios Ioannidis Problem based learning, Programming, Unix Bench biologists Clinical Scientists Graduate Students beginner bioinformaticians biocurators 2014-01-13 2017-10-09
Quality Control of Illumina Sequencing data in the cloud

This course was created as part of the SeqAhead project and contains both powerpoint presentations and course materials that teach students how to set up an Amazon EC2 virtual machine, connect to it, download some Illumina data from  the short read archive (SRA), assess the quality of the data...

Keywords: Bioinformatics, Cloud, Next generation sequencing, Next generation sequencing data analysis, Ngs bioinformatics, Qc ngs data

Quality Control of Illumina Sequencing data in the cloud https://tess.elixir-europe.org/materials/quality-control-of-illumina-sequencing-data-in-the-cloud This course was created as part of the SeqAhead project and contains both powerpoint presentations and course materials that teach students how to set up an Amazon EC2 virtual machine, connect to it, download some Illumina data from  the short read archive (SRA), assess the quality of the data and learn to trim both bad quality bases and adapter sequences Bioinformatics, Cloud, Next generation sequencing, Next generation sequencing data analysis, Ngs bioinformatics, Qc ngs data Beginners 2014-01-13 2017-10-09
RNA-seq Analysis 2014 Module 3 - Expression and Differential Expression

Get FPKM style expression estimates using Cufflinks Perform differential expression analysis with Cuffdiff Perform summary analysis with CummeRbund Downstream interpretation of expression analysis (multiple testing, clustering, heatmaps, classification, pathway analysis, etc.)  

Keywords: Differential expression, Transript expression

RNA-seq Analysis 2014 Module 3 - Expression and Differential Expression https://tess.elixir-europe.org/materials/rna-seq-analysis-2014-module-3-expression-and-differential-expression Get FPKM style expression estimates using Cufflinks Perform differential expression analysis with Cuffdiff Perform summary analysis with CummeRbund Downstream interpretation of expression analysis (multiple testing, clustering, heatmaps, classification, pathway analysis, etc.)   Differential expression, Transript expression Biologists, Genomicists, Computer Scientists Graduate Students Post-Doctoral Fellows Researchers 2014-06-27 2017-10-09
"Key-terms", a learning game for conceptual consolidation

A short presentation describing a new participatory method that easily engages course participants in gamestorming and helps to consolidate new knowledge, by collectively agreeing on ways to place concepts in mind maps. "Key-terms" is a classroom game that can be played in a variety of contexts,...

Keywords: Education, Instruction, Training

"Key-terms", a learning game for conceptual consolidation https://tess.elixir-europe.org/materials/key-terms-a-learning-game-for-conceptual-consolidation A short presentation describing a new participatory method that easily engages course participants in gamestorming and helps to consolidate new knowledge, by collectively agreeing on ways to place concepts in mind maps. "Key-terms" is a classroom game that can be played in a variety of contexts, from group discussions to several forms of Non-Formal learning (e.g. training) events. It helps a lot in engaging a small audience, following any wrap-up (recapitulation) session. Learners capture terms that they find conceptually important and contribute to a merged list. Then they are invited to pin these terms on a cork board in specified ways, such as placing related terms together (clustering), placing high order terms above lower order ones, etc. The instructor/facilitator can intervene at several levels in minimal, non-disturbing interventions, that help to contextualise what is happening. A depiction of how concepts are perceived in a class emerges quickly. This is, in practice, a visual concept map. The technique is being tested in the context of technical training provision, with the help of IT. Education, Instruction, Training Educators Ontologists Trainers 2014-07-06 2017-10-09
GOBLET Talk at INCoB 2014

Supporting Trainers to Improve Bioinformatics Education Globally Speaker: Dr. Michelle Brazas A needs assessment isn’t necessary to realize that across the globe, there is a high demand for quality bioinformatics training in all domains of life science. Delivering on this demand however is not...

Keywords: Goblet

GOBLET Talk at INCoB 2014 https://tess.elixir-europe.org/materials/goblet-talk-at-incob-2014 Supporting Trainers to Improve Bioinformatics Education Globally Speaker: Dr. Michelle Brazas A needs assessment isn’t necessary to realize that across the globe, there is a high demand for quality bioinformatics training in all domains of life science. Delivering on this demand however is not trivial. In addition to computational infrastructure and software tools, quality bioinformatics training depends upon excellent trainers and training resources. With a focus on the trainer in the learning equation, the Global Organization for Bioinformatics Learning, Education and Training (GOBLET) aims to facilitate the advancement of bioinformatics education globally by training and supporting a network of bioinformatics trainers. Activities include coordinating training efforts, sharing data sets and teaching materials, discussing best practices and building up teaching standards and teaching recognition. Examples to improve your bioinformatics training programs were provided. Through support and development of trainer excellence, GOBLET is working to improve the global landscape in bioinformatics education. Goblet General Interest 2014-08-14 2017-10-09
Understanding Multiple Sequence Alignments - Lecture Handouts & Utopia Hands-On

This presentation aims to provide a basic understanding of the range of contexts in which protein sequence alignments are used and are useful, focusing on the importance of sequence similarities and differences, and what they tell us. Overall, the objective is to offer different perspectives and...

Keywords: Multiple sequence alignment, Protein sequence analysis, Sequence analysis

Understanding Multiple Sequence Alignments - Lecture Handouts & Utopia Hands-On https://tess.elixir-europe.org/materials/understanding-multiple-sequence-alignments-lecture-handouts-utopia-hands-on This presentation aims to provide a basic understanding of the range of contexts in which protein sequence alignments are used and are useful, focusing on the importance of sequence similarities and differences, and what they tell us. Overall, the objective is to offer different perspectives and insights into the meaning of alignments, and to convey an understanding of the use of metaphor and its role in interpreting alignments. The talk is accompanied by a hands-on practical analysis using the Utopia sequence analysis suite. Multiple sequence alignment, Protein sequence analysis, Sequence analysis Biomedical researchers Life Science Researchers Postgraduate students 2013-12-17 2017-10-09
Introduction to Multiple Sequence Alignments (MSAs) and Phylogenies

Slides used for teaching an introduction to phylogenies and MSAs in the context of phylogenies for the first day of a two-day course on MSAs at Cambridge University, in the UK, in December 2013. Course taught together with Holger Dinkel and Terri Attwood.

Keywords: Molecular evolution, Multiple sequence alignment, Phylogenetics, Protein sequence analysis, Sequence alignment

Introduction to Multiple Sequence Alignments (MSAs) and Phylogenies https://tess.elixir-europe.org/materials/introduction-to-multiple-sequence-alignments-msas-and-phylogenies Slides used for teaching an introduction to phylogenies and MSAs in the context of phylogenies for the first day of a two-day course on MSAs at Cambridge University, in the UK, in December 2013. Course taught together with Holger Dinkel and Terri Attwood. Molecular evolution, Multiple sequence alignment, Phylogenetics, Protein sequence analysis, Sequence alignment Bench biologists Post-Doctoral Fellows Postgraduate students principle investigators 2013-12-19 2017-10-09
Flow Cytometry 2013 Module 1 - Introduction to Flow Cytometry Analysis in R

Lecture on how tools to be demonstrated have been used in research and clinical applications Description of data and underlying biological problem Summary of a typical work flow to be covered in the workshop: transformation, margin event removal, quality assurance, gating, recording and...

Keywords: Introduction flow cytometry, Review r

Flow Cytometry 2013 Module 1 - Introduction to Flow Cytometry Analysis in R https://tess.elixir-europe.org/materials/flow-cytometry-2013-module-1-introduction-to-flow-cytometry-analysis-in-r Lecture on how tools to be demonstrated have been used in research and clinical applications Description of data and underlying biological problem Summary of a typical work flow to be covered in the workshop: transformation, margin event removal, quality assurance, gating, recording and visualizing results Short R refresher of tools to be used Variable assignment, simple arithmetic Vectors, matrices, lists, names Functions, apply, sapply, lapply Simple plotting functions Introduction flow cytometry, Review r 2013-06-25 2017-10-09
Interpreting Phylogenies

Content for a one-day course delivered in April 2013 at the University of Cambridge, together with Sarah Parks, Cilia Antoniou, and Adrian Friday. Uses examples taken from several published papers to explore some of the assumptions and concepts we use when presenting and interpreting phylogenetic...

Keywords: Molecular evolution, Phylogenetics

Interpreting Phylogenies https://tess.elixir-europe.org/materials/interpreting-phylogenies Content for a one-day course delivered in April 2013 at the University of Cambridge, together with Sarah Parks, Cilia Antoniou, and Adrian Friday. Uses examples taken from several published papers to explore some of the assumptions and concepts we use when presenting and interpreting phylogenetic trees. Includes exercises on visulaising and manipulating trees using Dendroscope and NJplot to reinforce some of these ideas, while also providing a very basic overview of steps involved in a generic phylogenetic analysis. Molecular evolution, Phylogenetics experimeintal biologist researchers field biologist researchers 2013-09-13 2017-10-09
Workshop on Education in Bioinformatics 2014 - ISMB 2014 - Benjamin Good

Online Gaming in Bioinformatics: The ‘Fun’ Model to Online Bioinformatics Education Benjamin Good, Senior Staff Scientist, Department of Molecular and Experimental Medicine, The Scripps Research Institute. Dr. Good’s research focuses on games with a purpose in crowdsourcing biological knowledge....

Scientific topics: Bioinformatics

Keywords: Bioinformatics, Gaming, Online learning

Workshop on Education in Bioinformatics 2014 - ISMB 2014 - Benjamin Good https://tess.elixir-europe.org/materials/workshop-on-education-in-bioinformatics-2014-ismb-2014-benjamin-good Online Gaming in Bioinformatics: The ‘Fun’ Model to Online Bioinformatics Education Benjamin Good, Senior Staff Scientist, Department of Molecular and Experimental Medicine, The Scripps Research Institute. Dr. Good’s research focuses on games with a purpose in crowdsourcing biological knowledge. To this end, his research has contributed to the development of a suite of interactive video games that allow for play, learning and contributions to science: genegames.org. Dr. Good spoke on online games for bioinformatics and how they can (or cannot) be used to teach bioinformatics at WEB 2014 at ISMB 2014. Bioinformatics Bioinformatics, Gaming, Online learning General Interest 2014-08-14 2017-10-09
SV-Autopilot: the new face of Structural Variant Detection

Although there are many tools designed for structural variation (SV) analysis there has been no bench mark study to guide scientists in choosing the best tool for their particular data set. In this test case, AllBio Test Case 2 – Identification of Large Structural Variants, a researcher is eager...

Keywords: Ngs bioinformatics, Structural variations

SV-Autopilot: the new face of Structural Variant Detection https://tess.elixir-europe.org/materials/sv-autopilot-the-new-face-of-structural-variant-detection Although there are many tools designed for structural variation (SV) analysis there has been no bench mark study to guide scientists in choosing the best tool for their particular data set. In this test case, AllBio Test Case 2 – Identification of Large Structural Variants, a researcher is eager to identify large structural variants in multiple accessions of Arabidopsis. What is needed is a tool which can identify the type and quality of the sequencing reads, assemble them with regards to a reference genome, and predict large structural variants (>30nt). Lastly, it is desirable that the tool should have a user-friendly interface with which bench scientists can easily examine structural variants in their accession(s) of interest that need to be validated. Here we have not only created the benchmark data, but have also created a Virtual Machine framework that can automate this process using multiple SV prediction algorithms in parallel and refine the output to reduce false positive calls. We call this SV-AUTOPILOT, a Structural Variation AUTOmated PIpeLine Optimization Tool. This tool was tested by participants during the workshop. Link to SV-Autopilot: https://github.com/ALLBio/allbiotc2 Ngs bioinformatics, Structural variations Biologists bioinformaticians software developers, bioinformaticians 2014-09-10 2017-10-09
UPGMA Worked Example

A walkthrough of clustering 7 biological sequences (A-G) using the Unweighted Pair-Group Method with Arithmetic mean (UPGMA) method. Note that UPGMA is actually a generic method and thus the walkthrough could apply to any objects A-G for which pairwise distances can be calculated. A small CGI...

Keywords: Molecular phylogeny, Phylogenetics, Upgma

UPGMA Worked Example https://tess.elixir-europe.org/materials/upgma-worked-example A walkthrough of clustering 7 biological sequences (A-G) using the Unweighted Pair-Group Method with Arithmetic mean (UPGMA) method. Note that UPGMA is actually a generic method and thus the walkthrough could apply to any objects A-G for which pairwise distances can be calculated. A small CGI site for generating a UPGMA tree from a distance matrix can be found here. The source data for this worked example is a subset of Cytochrome C distances from Table 3 of one of the seminal phylogenetic papers: Fitch WM & Margoliash E (1967). Construction of phylogenetic trees. Science 155:279-84. PowerPoint timings are set up to be consistent with autolooping the presentation. Molecular phylogeny, Phylogenetics, Upgma Beginners 2014-08-04 2017-10-09
Workshop on Education in Bioinformatics 2014 - ISMB 2014 - Michael Love

MOOCs in Bioinformatics: The Online Course Model to Online Bioinformatics Education Dr. Michael Love, edX Faculty and post-doctoral fellow in Dept. Biostatistics, Dana Farber Cancer Institute and Harvard School of Public Health. Dr. Love teaches the Data Analysis for Genomics course offered...

Keywords: Bioinformatics, Mooc

Workshop on Education in Bioinformatics 2014 - ISMB 2014 - Michael Love https://tess.elixir-europe.org/materials/workshop-on-education-in-bioinformatics-2014-ismb-2014-michael-love MOOCs in Bioinformatics: The Online Course Model to Online Bioinformatics Education Dr. Michael Love, edX Faculty and post-doctoral fellow in Dept. Biostatistics, Dana Farber Cancer Institute and Harvard School of Public Health. Dr. Love teaches the Data Analysis for Genomics course offered through edX. His research focuses on inferring biologically meaningful patterns from high-throughput sequencing read counts. He also develops open-source statistical software for the analysis of exome sequencing and RNA sequencing experiments for the Bioconductor Project. Dr. Love spoke on how open online courses operate and how they can (or cannot) be used to teach bioinformatics at WEB 2014 at ISMB 2014. Bioinformatics, Mooc 2014-08-14 2017-10-09
Workshop on Education in Bioinformatics 2014 - ISMB 2014 - David Searls

Self-directed Learning: Does online learning in bioinformatics work? Proposed Speaker: (CONFIRMED) David Searls, Adjunct Associate Professor, Perleman School of Medicine, University of Pennsylvania. Dr. Searls is an independent consultant in bioinformatics and former Senior VP of Bioinformatics...

Keywords: Bioinformatics, Mooc, Online learning

Workshop on Education in Bioinformatics 2014 - ISMB 2014 - David Searls https://tess.elixir-europe.org/materials/workshop-on-education-in-bioinformatics-2014-ismb-2014-david-searls Self-directed Learning: Does online learning in bioinformatics work? Proposed Speaker: (CONFIRMED) David Searls, Adjunct Associate Professor, Perleman School of Medicine, University of Pennsylvania. Dr. Searls is an independent consultant in bioinformatics and former Senior VP of Bioinformatics at GlaxoSmithKline Pharmaceuticals. He recently authored “Ten Simple Rules for Online Learning” and “An Online Bioinformatics Curriculum”. Dr. Searls spoke on the opportunities and challenges of online learning in bioinformatics from a learner's perspective at WEB 2014 at ISMB 2014. Bioinformatics, Mooc, Online learning General Interest 2014-08-14 2017-10-09
Bioinformatics approaches to Identify causative sequence variants in farm animals

ALLBIO and DEANN Training course: 12-16 May 2014, SLU, Uppsala, Sweden The training is mainly intended for researchers that want or already use techniques like genome-wide SNP genotyping, candidate gene sequencing, transcriptome sequencing and other bioinformatics tools in their work to...

Keywords: Allbio, Farm animals, Qtl, Rnaseq, Transcription factors

Bioinformatics approaches to Identify causative sequence variants in farm animals https://tess.elixir-europe.org/materials/bioinformatics-approaches-to-identify-causative-sequence-variants-in-farm-animals ALLBIO and DEANN Training course: 12-16 May 2014, SLU, Uppsala, Sweden The training is mainly intended for researchers that want or already use techniques like genome-wide SNP genotyping, candidate gene sequencing, transcriptome sequencing and other bioinformatics tools in their work to elucidate the specific contributions of genetic and other factors in shaping the phenotypic variability in farm animals. Allbio, Farm animals, Qtl, Rnaseq, Transcription factors Biologists bioinformaticians geneticists 2014-11-17 2017-10-09
Evolutionary Bioinformatics Videos

"Evolution Academy" videos (http://post.queensu.ca/~forsdyke/videolectures.htm). The latter are a course of 54 short (15 min) videos that follow the style of Salman Khan's "Khan Academy," and are addressed primarily to High School and beginning College students. There are also several longer (1...

Scientific topics: Evolutionary biology

Keywords: Biohistory, Evolutionary bioinformatics, Evolutionary biology

Evolutionary Bioinformatics Videos https://tess.elixir-europe.org/materials/evolutionary-bioinformatics-videos "Evolution Academy" videos (http://post.queensu.ca/~forsdyke/videolectures.htm). The latter are a course of 54 short (15 min) videos that follow the style of Salman Khan's "Khan Academy," and are addressed primarily to High School and beginning College students. There are also several longer (1 h) conference presentations that bring together various themes. These are backed by several webpages (accessible by way of http://post.queensu.ca/~forsdyke/homepage.htm ), and a textbook, now in its third edition (2016; for Introduction see Materials File). Evolutionary biology Biohistory, Evolutionary bioinformatics, Evolutionary biology High School Students Researchers University and College Level Students 2017-04-04 2017-10-09
Found 0 upcoming events. Found 1 past event. View all results.