scripts, examples
Using OpenCloning to automate the planning and documentation of cloning and genome engineering via python scripting
A set of examples that show how to use OpenCloning to automate cloning via python scripting
OpenCloning is an Open-Source web application to plan and document cloning. Users can:
๐ก Import plasmid sequences from AddGene and gene sequences from NCBI.
๐ Load their own sequence files.
๐งฌ Plan cloning and design primers using common techniques (Gibson, golden gate, gateway, etc.).
๐ฆ Plan strain and cell line engineering via CRISPR and homologous recombination, with use-cases not supported by SnapGene or Benchling.
๐ค Automate repetitive cloning and primer design using scripts or web forms.
๐พ Download final constructs as GenBank or FASTA files.
๐ Archive the entire cloning history in an Open format and load it later.
๐ ๏ธ Create reusable cloning templates for cloning kits.
Licence: MIT License
Keywords: Synthetic biology, Genetic Engineering, Molecular biology
Resource type: scripts, examples
Status: Active
Prerequisites:
- Knowledge of python scripting
- Knowledge of molecular biology and cloning techniques
Scientific topics: Synthetic biology, Metabolic engineering, Molecular biology, Genetic engineering
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