scripts, examples

Using OpenCloning to automate the planning and documentation of cloning and genome engineering via python scripting

A set of examples that show how to use OpenCloning to automate cloning via python scripting

OpenCloning is an Open-Source web application to plan and document cloning. Users can:

๐Ÿ“ก Import plasmid sequences from AddGene and gene sequences from NCBI.
๐Ÿ“ Load their own sequence files.
๐Ÿงฌ Plan cloning and design primers using common techniques (Gibson, golden gate, gateway, etc.).
๐Ÿฆ  Plan strain and cell line engineering via CRISPR and homologous recombination, with use-cases not supported by SnapGene or Benchling.
๐Ÿค– Automate repetitive cloning and primer design using scripts or web forms.
๐Ÿ’พ Download final constructs as GenBank or FASTA files.
๐Ÿ“œ Archive the entire cloning history in an Open format and load it later.
๐Ÿ› ๏ธ Create reusable cloning templates for cloning kits.

Licence: MIT License

Keywords: Synthetic biology, Genetic Engineering, Molecular biology

Resource type: scripts, examples

Status: Active

Prerequisites:

  • Knowledge of python scripting
  • Knowledge of molecular biology and cloning techniques

Authors: Manuel Lera-Ramirez

Scientific topics: Synthetic biology, Metabolic engineering, Molecular biology, Genetic engineering

External resources:

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