e-learning, Video, Webinar

Using OpenCloning, an Open-Source web application to plan and document cloning and genome engineering

View material

A playlist of videos showing how to use OpenCloning. It contains a general introduction to the tool and short videos showing how to use it for particular tasks (e.g. designing primers for Gateway Cloning).

OpenCloning is an Open-Source web application to plan and document cloning. Users can:

  • 📡 Import plasmid sequences from AddGene and gene sequences from NCBI.
  • 📁 Load their own sequence files.
  • 🧬 Plan cloning and design primers using common techniques (Gibson, golden gate, gateway, etc.).
  • 🦠 Plan strain and cell line engineering via CRISPR and homologous recombination, with use-cases not supported by SnapGene or Benchling.
  • 🤖 Automate repetitive cloning and primer design using scripts or web forms.
  • 💾 Download final constructs as GenBank or FASTA files.
  • 📜 Archive the entire cloning history in an Open format and load it later.
  • 🛠️ Create reusable cloning templates for cloning kits.

Supports several techniques:

  • PCR
  • Restriction digestion
  • Ligation
  • Homologous recombination
  • Gibson assembly
  • Golden gate assembly
  • Gateway Cloning

DOI: https://doi.org/10.5281/zenodo.14882672

Keywords: web application, Genetic Engineering, Molecular biology, genome engineering, Genetics

Target audience: Biologists, Biotechnology Companies

Resource type: e-learning, Video, Webinar

Status: Active

Learning objectives:

  • Learn basics of OpenCloning - loading sequences, simulating cloning, designing primers, saving files- (introductory video)
  • Learn specific features (shorter more specific videos)

Date created: 2025-04-11

Authors: Manuel Lera-Ramirez

Scientific topics: Genetic engineering, Molecular biology, Synthetic biology, Metabolic engineering, Genetics

External resources:
OpenCloning
Activity log