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RNA-seq Analysis 2014 Module 2 - Alignment and Visualization

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Use of Bowtie/TopHat

        ‘Regular mode’ vs. ‘Fusion mode’

        Introduction to the BAM format

        Basic manipulation of BAMs with samtools, etc.

        Visualization of RNA-seq alignments - IGV

        BAM read counting and determination of variant allele expression status
 

 

Keywords: Data visualization, Rna seq alignment

Target audience: Biologists, Genomicists, Computer Scientists, Graduate Students, Post-Doctoral Fellows, Researchers

Authors: Michelle Brazas

Remote created date: 2014-06-27

Remote updated date: 2017-10-09

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