RNA-seq Analysis 2014 Module 2 - Alignment and Visualization

Use of Bowtie/TopHat

        ‘Regular mode’ vs. ‘Fusion mode’

        Introduction to the BAM format

        Basic manipulation of BAMs with samtools, etc.

        Visualization of RNA-seq alignments - IGV

        BAM read counting and determination of variant allele expression status
 

 

Keywords: Data visualization, Rna seq alignment

Target audience: Biologists, Genomicists, Computer Scientists, Graduate Students, Post-Doctoral Fellows, Researchers

Authors: Michelle Brazas

Remote created date: 2014-06-27

Remote updated date: 2017-10-09

RNA-seq Analysis 2014 Module 2 - Alignment and Visualization https://tess.elixir-europe.org/materials/rna-seq-analysis-2014-module-2-alignment-and-visualization Use of Bowtie/TopHat ‘Regular mode’ vs. ‘Fusion mode’ Introduction to the BAM format Basic manipulation of BAMs with samtools, etc. Visualization of RNA-seq alignments - IGV BAM read counting and determination of variant allele expression status     Data visualization, Rna seq alignment Biologists, Genomicists, Computer Scientists Graduate Students Post-Doctoral Fellows Researchers 2014-06-27 2017-10-09