Researcher Training Day for Life Scientists_ Rad-seq with Stacks in Galaxy

This session demonstrated how to use Stacks tools wrapped in Galaxy to process your RAD-seq (Restriction-site Associated DNA sequencing) data for population genomics. We started with raw sequencing reads and take them through quality-control, and de novo assembly steps. We then demonstrated the use of Stacks tools in Galaxy to call variants, build a variant catalogue and output filtered variant calls for downstream analysis.

We also touched on some of the options in molecular approach to RAD-seq library design and explained the important considerations for different research questions and species of interest.

This session included an introduction to the Galaxy platform.

Keywords: bioinformatics

Target audience: Biologists

Difficulty level: Beginner

Licence: Other (Attribution)

Authors: Vicky Schneider

Contributors: Pip Griffin, Sonika Tyagi

Researcher Training Day for Life Scientists_ Rad-seq with Stacks in Galaxy https://tess.elixir-europe.org/materials/researcher-training-day-for-life-scientists_-rad-seq-with-stacks-in-galaxy This materials are associated to the Galaxy Australasia Meeting 2017 kicks off on Friday 3rd February with the Researcher Training Day. This event targets life scientists working with Next-Generation Sequencing data, in particular RNA-Seq, RAD-Seq, and microbial genomes. Pip Griffin Sonika Tyagi bioinformatics Biologists