Researcher Training Day for Life Scientists_ Rad-seq with Stacks in Galaxy
This session demonstrated how to use Stacks tools wrapped in Galaxy to process your RAD-seq (Restriction-site Associated DNA sequencing) data for population genomics. We started with raw sequencing reads and take them through quality-control, and de novo assembly steps. We then demonstrated the use of Stacks tools in Galaxy to call variants, build a variant catalogue and output filtered variant calls for downstream analysis.
We also touched on some of the options in molecular approach to RAD-seq library design and explained the important considerations for different research questions and species of interest.
This session included an introduction to the Galaxy platform.
Target audience: Biologists
Difficulty level: Beginner
Licence: Other (Attribution)
Contributors: Pip Griffin, Sonika Tyagi