The O-QT Assistant application offers users a multi-agent interface to automate chemical hazard assessment, metabolism simulation, and read-across recommendations using the OECD QSAR Toolbox. Unlike standard manual workflows, this tool acts as an intelligent automation layer. It synthesizes data by employing a “Context → Specialists → Read-Across → Synthesis” agent workflow, connecting directly to a locally running instance of the QSAR Toolbox via its Web API.