e-learning

Indexing and profiling microbes with MetaSBT

Abstract

Introduction

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • What are Sequence Bloom Trees and how does MetaSBT use them for genomics?
  • How do you build a custom viral database from a set of reference genomes?
  • How can an existing MetaSBT database be updated with new viruses?
  • How do you profile a query viral genome against a database to determine its identity and novelty?
  • How can we interpret the viral genome profiling results?

Learning Objectives

  • Understand the fundamental concepts of MetaSBT for efficient genomic indexing.
  • Learn to use the metasbt_index tool to create a custom viral database.
  • Learn to use the metasbt_index tool to update an existing database.
  • Learn to use the metasbt_profile tool to identify and characterize a query viral genome.
  • Be able to interpret the similarity reports generated by the profiling tool.

Licence: Creative Commons Attribution 4.0 International

Keywords: Microbiome, diversity, metagenomics, taxonomic profiling

Target audience: Students

Resource type: e-learning

Version: 7

Status: Active

Prerequisites:

  • Galaxy Basics for everyone
  • Introduction to Galaxy Analyses

Learning objectives:

  • Understand the fundamental concepts of MetaSBT for efficient genomic indexing.
  • Learn to use the metasbt_index tool to create a custom viral database.
  • Learn to use the metasbt_index tool to update an existing database.
  • Learn to use the metasbt_profile tool to identify and characterize a query viral genome.
  • Be able to interpret the similarity reports generated by the profiling tool.

Date modified: 2025-08-25

Date published: 2025-08-25

Authors: Fabio Cumbo

Contributors: Daniel Blankenberg

Scientific topics: Metagenomics, Microbial ecology, Taxonomy

External resources:

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