e-learning

Hybrid genome assembly - Nanopore and Illumina

Abstract

Assemble a genome! Learn how to create and assess genome assemblies using the powerful combination of Nanopore and Illumina reads.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • How do long- and short-read assembly methods differ?

Learning Objectives

  • Understand how Nanopore and Illumina reads can be used together to produce a high-quality assembly
  • Become familiar with genome assembly and polishing programs
  • Learn how to assess the quality of a genome assembly, regardless of whether a reference genome is present or absent
  • Be able to assemble an unknown, previously undocumented genome to high-quality using Nanopore and Illumina reads!

Licence: Creative Commons Attribution 4.0 International

Keywords: Assembly, assembly, denovo, genomics, illumina, nanopore

Target audience: Students

Resource type: e-learning

Version: 1

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Quality Control

Learning objectives:

  • Understand how Nanopore and Illumina reads can be used together to produce a high-quality assembly
  • Become familiar with genome assembly and polishing programs
  • Learn how to assess the quality of a genome assembly, regardless of whether a reference genome is present or absent
  • Be able to assemble an unknown, previously undocumented genome to high-quality using Nanopore and Illumina reads!

Date modified: 2025-11-03

Date published: 2025-11-03

Authors: Grace Hall, Tristan Reynolds

Scientific topics: Sequence assembly


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