hands-on tutorial

Long non-coding RNAs (lncRNAs) annotation with FEELnc

The questions this addresses are:
- How to annotate lncRNAs with FEELnc?
- How to classify lncRNAs according to their localisation and direction of transcription of proximal RNA transcripts?
- How to update genome annotation with these annotated lncRNAs?

The objectives are:
- Load data (genome assembly, annotation and mapped RNASeq) into Galaxy
- Perform a transcriptome assembly with StringTie
- Annotate lncRNAs with FEELnc
- Classify lncRNAs according to their location
- Update genome annotation with lncRNAs

Licence: Creative Commons Attribution 4.0 International

Keywords: Genome Annotation, eukaryote

Target audience: Students

Resource type: hands-on tutorial

Version: 9

Status: Active

Prerequisites:

  • Genome annotation with Funannotate
  • Introduction to Galaxy Analyses

Learning objectives:

Load data (genome assembly, annotation and mapped RNASeq) into Galaxy
- Perform a transcriptome assembly with StringTie
- Annotate lncRNAs with FEELnc
- Classify lncRNAs according to their location
- Update genome annotation with lncRNAs

Date modified: 2024-09-03

Date published: 2022-09-23

Authors: Stéphanie Robin

Contributors: Anthony Bretaudeau


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