e-learning

Genome assembly using PacBio data

Abstract

In this tutorial, we will assemble a genome of a species of fungi in the family Aspergillus, Aspergillus niger, from PacBio sequencing data. These data will be downloaded from ENA. The quality of the assembly obtained will be analyzed, in particular by comparing it to a reference assembly, available on ENA.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • How to perform a genome assembly with PacBio data ?
  • How to check assembly quality ?

Learning Objectives

  • Assemble a Genome with PacBio data
  • Assess assembly quality

Licence: Creative Commons Attribution 4.0 International

Keywords: Assembly, assembly, biodiversity, pacbio

Target audience: Students

Resource type: e-learning

Version: 2

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Quality Control

Learning objectives:

  • Assemble a Genome with PacBio data
  • Assess assembly quality

Date modified: 2025-06-04

Date published: 2021-11-29

Authors: Alexandre Cormier, Anthony Bretaudeau, Erwan Corre, Laura Leroi, Solenne Correard, Stéphanie Robin

Contributors: Anthony Bretaudeau, Björn Grüning, Bérénice Batut, Cristóbal Gallardo, Helena Rasche, Saskia Hiltemann, Solenne Correard, Stéphanie Robin

Scientific topics: Sequence assembly, Genomics, Biodiversity


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