e-learning
Genome Assembly of a bacterial genome (MRSA) sequenced using Illumina MiSeq Data
Abstract
Sequencing (determining of DNA/RNA nucleotide sequence) is used all over
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- How to check the quality of the MiSeq data?
- How to perform an assembly of a bacterial genome with MiSeq data?
- How to check the quality of an assembly?
Learning Objectives
- Run tools to evaluate sequencing data on quality and quantity
- Process the output of quality control tools
- Improve the quality of sequencing data
- Run a tool to assemble a bacterial genome using short reads
- Run tools to assess the quality of an assembly
- Understand the outputs of tools to assess the quality of an assembly
Licence: Creative Commons Attribution 4.0 International
Keywords: Assembly, assembly, illumina, microgalaxy
Target audience: Students
Resource type: e-learning
Version: 17
Status: Active
Prerequisites:
- Introduction to Galaxy Analyses
- Quality Control
Learning objectives:
- Run tools to evaluate sequencing data on quality and quantity
- Process the output of quality control tools
- Improve the quality of sequencing data
- Run a tool to assemble a bacterial genome using short reads
- Run tools to assess the quality of an assembly
- Understand the outputs of tools to assess the quality of an assembly
Date modified: 2025-11-18
Date published: 2021-03-24
Contributors: Anthony Bretaudeau, Bazante Sanders, Björn Grüning, Bérénice Batut, Cristóbal Gallardo, Deepti Varshney, Helena Rasche, Miaomiao Zhou, Nate Coraor, Paul Zierep, Saskia Hiltemann, Teresa Müller, Verena Moosmann, Wolfgang Maier, pimarin
Scientific topics: Sequence assembly, Whole genome sequencing, Public health and epidemiology, Genomics, Microbiology
Activity log
