e-learning

Formation of the Super-Structures on the Inactive X

Abstract

Within a cell nucleus, the DNA is tightly-packed and the chromatin is spatially distributed with different levels and scales of organizations.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • Histone modification?
  • How is a raw set of ChIP-seq data processed and analyzed?
  • Where are the enriched regions for H3K27me3, H3K4me3 and CTCF on the chrX?

Learning Objectives

  • Inspect the read quality
  • Trim low quality bases
  • Map reads on a reference genome
  • Assess the quality of a ChIP-seq experiment
  • Extract coverage files
  • Call enriched regions or peaks

Licence: Creative Commons Attribution 4.0 International

Keywords: ChIP-seq, Epigenetics

Target audience: Students

Resource type: e-learning

Version: 15

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Mapping
  • Quality Control

Learning objectives:

  • Inspect the read quality
  • Trim low quality bases
  • Map reads on a reference genome
  • Assess the quality of a ChIP-seq experiment
  • Extract coverage files
  • Call enriched regions or peaks

Date modified: 2025-12-18

Date published: 2018-09-17

Authors: Anika Erxleben, Bérénice Batut, Fidel Ramirez, Friederike Dündar, Leily Rabbani, Pavankumar Videm, Vivek Bhardwaj

Contributors: Anup Kumar, Armin Dadras, Björn Grüning, Bérénice Batut, Cristóbal Gallardo, Florian Heyl, Gildas Le Corguillé, Helena Rasche, Joachim Wolff, Leily Rabbani, Martin Čech, Pavankumar Videm, Saskia Hiltemann, Tristan Reynolds

Scientific topics: Epigenomics


Activity log