e-learning
Detection and quantitation of N-termini (degradomics) via N-TAILS
Abstract
N-Tails is a special Proteomics technique to analyze peptide abundancy changes of protein N-termini. Prior to the MS measurement, N-Tails enriches unmodified, as well as acetylated N-termini. Both common and "unusual" N-termini are identified, where "unusual" means that the protein N-terminus was changes.
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- How can protein N-termini be enriched for LC-MS/MS?
- How to analyze the LC-MS/MS data?
Learning Objectives
- Run an N-TAILS data analysis.
Licence: Creative Commons Attribution 4.0 International
Keywords: Proteomics
Target audience: Students
Resource type: e-learning
Version: 15
Status: Active
Prerequisites:
Introduction to Galaxy Analyses
Learning objectives:
- Run an N-TAILS data analysis.
Date modified: 2023-05-16
Date published: 2017-02-14
Contributors: Anton Nekrutenko, Björn Grüning, Bérénice Batut, Clemens Blank, Florian Christoph Sigloch, Helena Rasche, Melanie Föll, Niall Beard, Nicola Soranzo, Saskia Hiltemann, William Durand
Scientific topics: Proteomics
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