e-learning

Detection and quantitation of N-termini (degradomics) via N-TAILS

Abstract

N-Tails is a special Proteomics technique to analyze peptide abundancy changes of protein N-termini. Prior to the MS measurement, N-Tails enriches unmodified, as well as acetylated N-termini. Both common and "unusual" N-termini are identified, where "unusual" means that the protein N-terminus was changes.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • How can protein N-termini be enriched for LC-MS/MS?
  • How to analyze the LC-MS/MS data?

Learning Objectives

  • Run an N-TAILS data analysis.

Licence: Creative Commons Attribution 4.0 International

Keywords: Proteomics

Target audience: Students

Resource type: e-learning

Version: 15

Status: Active

Prerequisites:

Introduction to Galaxy Analyses

Learning objectives:

  • Run an N-TAILS data analysis.

Date modified: 2023-05-16

Date published: 2017-02-14

Authors: Björn Grüning, Florian Christoph Sigloch

Contributors: Anton Nekrutenko, Björn Grüning, Bérénice Batut, Clemens Blank, Florian Christoph Sigloch, Helena Rasche, Melanie Föll, Niall Beard, Nicola Soranzo, Saskia Hiltemann, William Durand

Scientific topics: Proteomics

External resources:

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