e-learning

3: RNA-seq genes to pathways

Abstract

Sometimes there is quite a long list of genes to interpret after a differential expression analysis, and it is usually infeasible to go through the list one gene at a time trying to understand it’s biological function. A common downstream procedure is gene set testing, which aims to understand which pathways/gene networks the differentially expressed genes are implicated in. There are many different gene set testing methods that can be applied and it can be useful to try several.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • What are the differentially expressed pathways in the mammary gland of pregnant versus lactating mice?

Learning Objectives

  • Identification of differentially expressed pathways

Licence: Creative Commons Attribution 4.0 International

Keywords: Transcriptomics, mouse

Target audience: Students

Resource type: e-learning

Version: 13

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Mapping
  • Quality Control

Learning objectives:

  • Identification of differentially expressed pathways

Date modified: 2023-11-09

Date published: 2018-12-31

Authors: Belinda Phipson, Maria Doyle

Contributors: Björn Grüning, Helena Rasche, Nicola Soranzo

Scientific topics: Transcriptomics


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