e-learning

Genome annotation with Braker3

Abstract

Annotating the eukaryotic genome represents a somewhat more complex challenge than that of prokaryotes, mainly due to the generally larger size of eukaryotic genomes and their greater number of genes. This annotation can be carried out at different levels of precision, ranging from simple identification of coding and non-coding parts to detailed structural labeling, including for example the precise location of exons, introns and other regulatory elements.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • How to annotate an eukaryotic genome with Braker3?
  • How to evaluate and visualize annotated genomic features?

Learning Objectives

  • Load genome into Galaxy
  • Annotate genome with Braker3
  • Evaluate annotation quality with BUSCO
  • View annotations in JBrowse

Licence: Creative Commons Attribution 4.0 International

Keywords: Braker3, Genome Annotation, eukaryota, jbrowse1

Target audience: Students

Resource type: e-learning

Version: 1

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Masking repeats with RepeatMasker

Learning objectives:

  • Load genome into Galaxy
  • Annotate genome with Braker3
  • Evaluate annotation quality with BUSCO
  • View annotations in JBrowse

Date modified: 2025-03-17

Date published: 2025-03-17

Authors: Anthony Bretaudeau, Romane LIBOUBAN

Contributors: Deepti Varshney, Saskia Hiltemann

Scientific topics: Sequence assembly, Genomics


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