Flow Cytometry 2013 Module 3 - Preprocessing and Quality Assurance of FCM Data

Preprocessing

            Removing margin events

            Data transformation: log vs. biexponential

            Data normalization


    Quality Assurance

            Overview of quality assurance concepts: total raw/viable cell count, margin event count, outlier detection based on density of common parameters

            Building quality assurance objects using flowQ and generating summary HTML reports

Keywords: Flow cytometry data, Preprocessing, Quality assurance

Authors: Michelle Brazas

Remote created date: 2013-06-26

Remote updated date: 2017-10-09

Flow Cytometry 2013 Module 3 - Preprocessing and Quality Assurance of FCM Data https://tess.elixir-europe.org/materials/flow-cytometry-2013-module-3-preprocessing-and-quality-assurance-of-fcm-data Preprocessing Removing margin events Data transformation: log vs. biexponential Data normalization Quality Assurance Overview of quality assurance concepts: total raw/viable cell count, margin event count, outlier detection based on density of common parameters Building quality assurance objects using flowQ and generating summary HTML reports Flow cytometry data, Preprocessing, Quality assurance 2013-06-26 2017-10-09