e-learning

Clinical Metaproteomics 3: Verification

Abstract

In proteomic research, the primary goal is to obtain accurate and meaningful insights into the proteome of a biological system. Verifying the presence of peptides or proteins is a critical step in achieving this goal, ensuring the quality and reliability of the data and the biological relevance of the findings. This tutorial is a sequel to the clinical metaproteomics discovery workflow. Once you have identified microbial peptides, the next step is to verify these peptides, for which we use PepQuery.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • Why do we need to verify our identified peptides
  • What is the importance of making a new database for quantification

Learning Objectives

  • Verification of peptides helps in confirming the presence of the peptides in our samplle

Licence: Creative Commons Attribution 4.0 International

Keywords: Microbiome, label-TMT11

Target audience: Students

Resource type: e-learning

Version: 0

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Proteomics

Learning objectives:

  • Verification of peptides helps in confirming the presence of the peptides in our samplle

Date modified: 2025-08-20

Date published: 2025-08-20

Authors: Dechen Bhuming, Katherine Do, Subina Mehta

Contributors: Pratik Jagtap, Timothy J. Griffin

Scientific topics: Metagenomics, Microbial ecology


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