e-learning

16S Microbial Analysis with mothur (short)

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Abstract

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • What is the effect of normal variation in the gut microbiome on host health?

Learning Objectives

  • Analyze of 16S rRNA sequencing data using the mothur toolsuite in Galaxy
  • Using a mock community to assess the error rate of your sequencing experiment
  • Visualize sample diversity using Krona and Phinch

Licence: Creative Commons Attribution 4.0 International

Keywords: 16S, Microbiome, metabarcoding, microgalaxy

Target audience: Students

Resource type: e-learning

Version: 6

Status: Active

Prerequisites:

Introduction to Galaxy Analyses

Learning objectives:

  • Analyze of 16S rRNA sequencing data using the mothur toolsuite in Galaxy
  • Using a mock community to assess the error rate of your sequencing experiment
  • Visualize sample diversity using Krona and Phinch

Date modified: 2025-12-18

Date published: 2019-05-13

Authors: Bérénice Batut, Dave Clements, Saskia Hiltemann

Contributors: Anna Syme, Bérénice Batut, Helena Rasche, Igor Makunin, Michael Thang, Saskia Hiltemann, Tristan Reynolds

Scientific topics: Metagenomics, Microbial ecology, Taxonomy, Sequence analysis, Metabarcoding

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