Date: No date given

Familiarize yourself with the Galaxy user interface and its most important features,
then move on to analysis of genomic interval and deep-sequencing data
with tools and tool suites like MACS2, bedtools and deepTools.

Keywords: beginner, epigenetics, genomic-intervals

Learning objectives:

  • Apply an enrichment analysis and a robust peak detection
  • Apply appropriate analysis and quality control steps for ATAC-Seq
  • Apply appropriate analysis and quality control steps for CUT&RUN
  • Assess the quality of a ChIP-seq experiment
  • Call enriched regions or peaks
  • Extract coverage files
  • Familiarize yourself with the basics of Galaxy
  • Find protein binding motifs
  • Generate a heatmap of transcription start site accessibility
  • Get a first intuition what are common pitfalls.
  • Inspect the read quality
  • Learn how histories work
  • Learn how to analyse methylation data
  • Learn how to create a workflow
  • Learn how to extract and run a workflow
  • Learn how to obtain data from external sources
  • Learn how to run tools
  • Learn how to share a history
  • Learn how to share your work
  • Learn how to upload a file
  • Learn how to use a tool
  • Learn how to view histories
  • Learn how to view results
  • Map reads on a reference genome
  • Trim low quality bases
  • Visualise peaks for specific regions

Event types:

  • Workshops and courses

Sponsors: Australian BioCommons, ELIXIR Europe, Erasmus Medical Center, The Pennsylvania State University, University of Freiburg, de.NBI

Scientific topics: Epigenomics


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