Start: Tuesday, 27 March 2018 @ 08:30

End: Tuesday, 27 March 2018 @ 16:00

Contact: bioinformatics-core@sheffield.ac.uk

Venue: Pam Liversidge Design Studio 1 - D06

City: Sheffield

Country: United Kingdom

Postcode: S1 3JD

Description:

High-throughput RNA-sequencing is now the standard technique for quantifying transcript abundance in a biological sample of interest. In this course we will describe the processes that take place once you submit a library for RNA sequencing, and what data you should expect to receive from the Bioinformatics Core. We will describe the steps involved to go from sequencing library to a list of genes that show statistically significant differences between your biological conditions of interest. Practical sessions will use the user-friendly Galaxy interface (https://usegalaxy.org/) to demonstrate tasks such as alignment, quality control and assessing differential expression. We will also showcase some web sites you can use for enrichment and pathways analysis.

Event type:
  • Workshops and courses
Introduction to RNA-seq https://tess.elixir-europe.org/events/introduction-to-rna-seq High-throughput RNA-sequencing is now the standard technique for quantifying transcript abundance in a biological sample of interest. In this course we will describe the processes that take place once you submit a library for RNA sequencing, and what data you should expect to receive from the Bioinformatics Core. We will describe the steps involved to go from sequencing library to a list of genes that show statistically significant differences between your biological conditions of interest. Practical sessions will use the user-friendly Galaxy interface (https://usegalaxy.org/) to demonstrate tasks such as alignment, quality control and assessing differential expression. We will also showcase some web sites you can use for enrichment and pathways analysis. 2018-03-27 08:30:00 UTC 2018-03-27 16:00:00 UTC Pam Liversidge Design Studio 1 - D06, Sheffield, United Kingdom Pam Liversidge Design Studio 1 - D06 Sheffield United Kingdom S1 3JD [] bioinformatics-core@sheffield.ac.uk [] [] workshops_and_courses [] []