Introduction to Next Generation Sequencing Bioinformatics Workshop

MARCH31, 2017 (08:30-17:00)

Lokaal H04.212, ON2, Campus Gasthuisberg, KU Leuven, Leuven

Preliminary Program

08:30 – 9:00 - Welcome coffee

09:00 – 09:05 – Welcome by Prof. Joris Vermeesch

09:05 - 10:00 - Overview of NGS sequencing methods and technologies

Session lead: Jeroen Van Houdt - Genomics Core Leuven

10:00 - 11:00 – Understanding NGS raw data: Fastq format, quality checking, trimming, adapter clipping

Session lead: Koen Herten - Genomics Core Leuven

11:00 – 11:30 – Coffee break

11:30 – 12:30 – Read mapping and alignment: SAM format, alignment tools, visualization on IGV

Session lead: Koen Herten - Genomics Core Leuven

12:30 – 13:30 – Lunch

13:30 – 15:00 – Variant calling: tools, GATK, quality scores, baserecalibration, VCF files

Session lead: Erika Souche & Luc Dehaspe - Genomics Core Leuven

15:00 – 15:30 – Coffee break

15:30 – 17:00 – RNA-Seq data: Differential expression principles, counting, DE table interpretation

Session lead: Alvaro Cortés Calabuig – Genomics Core Leuven

Places are limited and are assigned on a first-come first-serve policy. The workshop is free of charge for students and members of academic institutions in Belgium. The cost for members of non-academic institutions (VIB, IMEC, pharma, etc.) is 100€. Sandwiches will be offered at lunch time. Late cancellations are subject to an administrative fee of 50€. Participants are required to bring their own laptops. For inquiries, please send an email to alvaro.cortes@uzleuven.be.