QC or not QC Data is the question

Sequence processing Issues

General sequence quality

  • 5’ base bias
  • 3’ quality decline

Library diversity

  • Low complexity sequence
  • Over-amplification

Contamination

  • Intrinsic to sample?
  • Cross contamination?
  • Sequencing adaptors?

Scientific topics: Quality affairs

Keywords: IBERS, Institute of Biological, Environmental and Rural Sciences

Authors: Dan Smith

QC or not QC Data is the question https://tess.elixir-europe.org/materials/qc-or-not-qc-data-is-the-question Sequencing QC using FastQC and Trimmomatic in Galaxy Quality affairs IBERS, Institute of Biological, Environmental and Rural Sciences