QC or not QC Data is the question

Sequence processing Issues

General sequence quality

  • 5’ base bias
  • 3’ quality decline

Library diversity

  • Low complexity sequence
  • Over-amplification


  • Intrinsic to sample?
  • Cross contamination?
  • Sequencing adaptors?

Keywords: IBERS, Institute of Biological, Environmental and Rural Sciences

Authors: Dan Smith

Scientific topics: Quality affairs

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