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Biodiversity Genomics Europe + SciLifeLab HiC Course

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This course aims to provide open, sustainable training in laboratory preparation of Hi-C libraries for reference genome sequencing of non-model organisms for BGE partner sequencing centers.

There are three course streams available:

  1. Introductory HiC Technique Course Stream - designed for those without previous experience in the HiC technique, this stream follows a group of researchers performing HiC for the first time.

  2. Challenging Samples HiC Course Stream - designed for those with some or advanced experience in the HiC technique, this stream follows a group of expert researchers as they troubleshoot and prepare challenging sample types.

  3. AssemblyLink HiC Course Stream - designed for those with some or advanced experience in the HiC technique, this stream follows a group of experienced researchers as they attempt and troubleshoot the AssemblyLink technique with a variety of sample types.

DOI: https://doi.org/10.5281/zenodo.14643653

Licence: Creative Commons Attribution 4.0 International

Contact: olga.pettersson@igp.uu.se

Keywords: HiC, Genome Assembly, Genome Sequencing, Epigenomics, Laboratory Technique

Target audience: Researchers, Bioinformaticians

Resource type: Video

Version: v.1-full

Status: Active

Prerequisites:

Participants must have:

  • an understanding of fundamental genomics and epigenomics concepts
  • the ability to demonstrate basic laboratory technique, including safe laboratory practices

Participants should have:
- an understanding of their local permit and safety requirements for processing and handling samples and reagents for HiC.

Learning objectives:

By the end of the course, participants will be able to:

  • independently prepare HiC libraries of low-complexity samples.
  • prepare HiC libraries at their home labs.
  • understand the most crucial steps of the protocol
  • troubleshoot crucial steps of the protocol in their home labs when applied to high-complexity samples

Date created: 2025-06-09

Date published: 2025-08-31

Authors: Maria Laura Aguilera Gil, Lorenzo Ballini, Laurie Bertrand, Simona Buonanno, Eunkyong Choi, Annunziata De Luisi, Giada Ferrari, Leonardo Girlanda, Carmela Gissi, Maria Gyftea, Brian Kilburn, Karine Labadie, Tuuli Lundback-Lärva, Graeme Oatley, Fernando Cruz Rodríguez, Irene Fabiola Roman Maldonado, Lily Shiue, Morten Skage, Pamela Veneziano Broccia, Ave Tooming-Klunderud, Olga Vinnere Pettersson

Contributors: Kristen Schroeder, Jill Jaworski, Hampus Pehrsson Ternström

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