Analysing Mapped Sequence Data with SeqMonk

SeqMonk is a program which can analyse large data sets of mapped genomic positions. It is most commonly used to work with data coming from high-throughput sequencing pipelines.

The program allows you to view your reads against an annotated genome and to quantitate and filter your data to let you identify regions of interest. It is a friendly way to explore and analysis very large datasets.

This course provides an introduction to the main features of SeqMonk and will run through the anlaysis of a couple of different datasets to show what sort of analysis options it provides.

Course content

  • What is SeqMonk
  • Starting and configuring the program
  • Creating a project and importing data
  • Using the chromosome viewer
  • Quantitating and Filtering Data
  • Creating Reports
  • Using Quantitation Pipelines
  • Correcting and Normalising Quantitations
  • Scaling analysis to larger studies
  • Running statistical tests
  • Interacting with external programs
  • Exporting text and graphics

Course Material

Scientific topics: Sequence alignment

Keywords: Babraham Institute

Difficulty level: Beginner

Licence: Other (Non-Commercial)

Authors: Simon Andrews

Analysing Mapped Sequence Data with SeqMonk https://tess.elixir-europe.org/materials/analysing-mapped-sequence-data-with-seqmonk SeqMonk is a program which can analyse large data sets of mapped genomic positions. It is most commonly used to work with data coming from high-throughput sequencing pipelines. The program allows you to view your reads against an annotated genome and to quantitate and filter your data to let you identify regions of interest. It is a friendly way to explore and analysis very large datasets. This course provides an introduction to the main features of SeqMonk and will run through the anlaysis of a couple of different datasets to show what sort of analysis options it provides. Sequence alignment Babraham Institute