This isn’t strictly necessary, but if you want to infer trajectories - pseudotime relationships between cells - you can try out these tutorials with the same dataset. Again, you get two options for inferring trajectories, and you can choose either.

Time estimation: 5 hours

Learning objectives:

• Execute multiple plotting methods designed to identify lineage relationships between cells
• Interpret these plots
• Identify which operations to perform on an AnnData object to obtain the files needed for Monocle
• Follow the Monocle3 workflow and choose the right parameter values
• Compare the outputs from Scanpy and Monocle
• Interpet trajectory analysis results