Organizer: Anna Marabotti (University of Salerno and ELIXIR-IIB Training Team, Italy), Roberto Tagliaferri (University of Salerno, Italy), Alessandro Weisz (University of Salerno, Italy), Loredana Le Pera (ELIXIR-IIB Training Team and IIT, Italy), Allegra Via (ELIXIR-IIB Training Coordinator, CNR-IBPM, Italy)

Host institution: Campus di Fisciano, University of Salerno, Italy

Start: Wednesday, 27 September 2017 @ 09:15

End: Friday, 29 September 2017 @ 16:30

Timezone: CET

Sponsors: ELIXIR Italy

Contact: elixir.ita.training@gmail.com, amarabotti@unisa.it

Venue: Campus di Fisciano, Università degli Studi di Salerno, Via Giovanni Paolo II, n. 132

City: Fisciano

County: Provincia di Salerno

Country: Italy

Postcode: 84084

Scientific topic: Transcriptomics, RNA splicing, Sequencing, Bioinformatics, RNA-Seq, Gene expression

Target audience:
  • PhD students and young researchers in the life science and computational biology field who are planning to use RNA-seq data and are looking for the best practices to analyze these types of data
Description:

Next-generation sequencing (NGS) of RNA libraries (RNA-Seq) has become increasingly common and it largely replaced microarray technology for gene expression profiling. The aim of this course is to get a deeper understanding of RNA-Seq experiments, providing a theoretical introduction to the data processing steps, together with practical sessions illustrating the use of the most popular data analysis tools. The classroom size is limited to 25 participants to optimize the learning and the interaction with the instructors. Starting from the raw sequenced data coming from different phenotypical samples (e.g disease vs healthy control samples), genes which are differentially expressed between the two conditions are determine. Some strategies are illustrated for detecting alternative splicing products, predicting novel isoforms and gene fusion events. Some methods for downstream analysis are described to give insight into how biological knowledge can be generated from RNA-Seq experiments. A lecture on single-cell RNA-Seq will provide an idea of how transcriptome data from individual cells is now emerging as a powerful tool, allowing the study of cell-to-cell gene expression heterogeneity in the same tissue.

Event type:
  • Workshops and courses

Capacity: 25

Eligibility:
  • First come first served
  • Registration of interest

Keywords: NGS, RNA-Seq, Bioinformatics, Data analysis, High Throughput Sequencing Analysis, Gene Expression

Training Course on Best practices for RNA-Seq data analysis https://tess.elixir-europe.org/events/training-course-on-best-practices-for-rna-seq-data-analysis Next-generation sequencing (NGS) of RNA libraries (RNA-Seq) has become increasingly common and it largely replaced microarray technology for gene expression profiling. The aim of this course is to get a deeper understanding of RNA-Seq experiments, providing a theoretical introduction to the data processing steps, together with practical sessions illustrating the use of the most popular data analysis tools. The classroom size is limited to 25 participants to optimize the learning and the interaction with the instructors. Starting from the raw sequenced data coming from different phenotypical samples (e.g disease vs healthy control samples), genes which are differentially expressed between the two conditions are determine. Some strategies are illustrated for detecting alternative splicing products, predicting novel isoforms and gene fusion events. Some methods for downstream analysis are described to give insight into how biological knowledge can be generated from RNA-Seq experiments. A lecture on single-cell RNA-Seq will provide an idea of how transcriptome data from individual cells is now emerging as a powerful tool, allowing the study of cell-to-cell gene expression heterogeneity in the same tissue. 2017-09-27 09:15:00 UTC 2017-09-29 16:30:00 UTC Anna Marabotti (University of Salerno and ELIXIR-IIB Training Team, Italy), Roberto Tagliaferri (University of Salerno, Italy), Alessandro Weisz (University of Salerno, Italy), Loredana Le Pera (ELIXIR-IIB Training Team and IIT, Italy), Allegra Via (ELIXIR-IIB Training Coordinator, CNR-IBPM, Italy) Campus di Fisciano, Università degli Studi di Salerno, Via Giovanni Paolo II, n. 132, Fisciano, Italy Campus di Fisciano, Università degli Studi di Salerno, Via Giovanni Paolo II, n. 132 Fisciano Provincia di Salerno Italy 84084 Transcriptomics RNA splicing Sequencing Bioinformatics RNA-Seq Gene expression Campus di Fisciano, University of Salerno, Italy elixir.ita.training@gmail.com, amarabotti@unisa.it ELIXIR Italy PhD students and young researchers in the life science and computational biology field who are planning to use RNA-seq data and are looking for the best practices to analyze these types of data 25 workshops_and_courses first_come_first_servedregistration_of_interest NGSRNA-SeqBioinformaticsData analysisHigh Throughput Sequencing AnalysisGene Expression