United Kingdom
RNA-seq gene expression and pathway analysis
Date: 9 - 11 June 2026
Timezone: London
Language of instruction: English
RNA-Seq (AKA whole transcriptomic shotgun sequencing) aims to determine the presence and quantity of RNA in a biological sample at a given moment in time. This allows you to determine the level of expression of these genes and therefore compare these expressions across different sample groups, i.e. Differential Gene Expression (DGE). At the end of this course you will be able to align the transcriptomes of samples to a reference genome to determine gene expression levels. Following this you will be able to carry out Differential Gene Expression analysis to determine which genes are up and down regulated between sample groups.
Places are restricted to UK-based researchers and only 30 places are available so please only register if you intend on attending.
The workshop will be held over two days on Tuesday 9 and Thursday 11 June 2026, 10am-4pm, with registration opening 9.30am on Tuesday.
The deadline to register is Monday 25 May noon, 2 weeks before the course. You will hear later that week whether you have been invited and will need to confirm your attendance.
Register to NEOF mailing list to hear first about future NEOF free bioinformatics courses: https://neof.org.uk/news/
Venue: United Kingdom
City: Online
Learning objectives:
At the end of the course learners will be able to:
- Quality control Illumina RNA-seq data.
- Map RNA-seq data to a prepared reference transcriptome.
- Visualise the mapping with IGV.
- Count the number of reads per gene and exon with htseq.
- Carry out differential gene expression analysis (DGE) with DESeq2.
- Visualise DGE results via MA plots, volcano plots, heatmaps, and PCA plots.
- Carry out Gene Ontology enrichment analysis.
Event types:
- Workshops and courses
Sponsors: NERC
Activity log
