NEOF - eDNA wetlab and bioinformatics IN PERSON Training
Date: 8 - 12 September 2025
Timezone: London
Language of instruction: English
Generating DNA sequence barcodes from mixed sources of DNA is an increasingly-used and efficient way to survey biodiversity, whether monitoring aquatic species by sampling water-derived DNA, characterising communities from soil samples, or analysing diet from faecal-derived DNA. This course provides an overview of metabarcoding with different barcoding genes to target particular taxa, and consists of a technical wet lab component and a bioinformatics component. Interested delegates may apply to attend either or both workshops.
During the wet lab component, we will give an overview of experimental design, head outside for a field sampling demonstration and return to the laboratory for a full course on the metabarcoding process. The laboratory techniques will include DNA extraction, polymerase chain reaction (PCR), gel electrophoresis, magnetic bead cleaning and quantification. Finally, we will outline the different platforms that are typically used for sequencing.
In the bioinformatics component, we will use an example data set to go from raw sequence data through to assigning taxonomy to identified sequence variants. We will then conduct typical downstream analyses to quantify and examine differences in diversity.
Register by Monday 21/7/25
Venue: Full location will be shared with invited participants. There will also be an opportunity to tour the NEOF Visitor Facility labs and hear about how they can be accessed for your research.
City: Sheffield
Country: United Kingdom
Organizer: NEOF
Event types:
- Workshops and courses
Tech requirements:
You will need to bring your own laptop, but no special software is required - just a web browser.
Cost basis: Cost incurred by all
Cost: £ 110.0 (GBP)
Sponsors: NERC
Activity log