Introduction to RNA-seq and ChIP-seq data analysis
Organizer: University of Cambridge
Host institution: University of Cambridge Bioinformatics Training
Start: Thursday, 14 May 2015 @ 08:30
End: Friday, 15 May 2015 @ 15:30
Venue: Craik-Marshall Building
Country: United Kingdom
Postcode: CB2 3ARTarget audience:
- Graduate students
- Postdocs and Staff members from the University of Cambridge
- Institutions and other external Institutions or individuals
The aim of this course is to familiarise the participants with the primary analysis of datasets generated through two popular high-throughout sequencing (HTS) assays: ChIP-seq and RNA-seq. This course starts with a brief introduction to the transition from capillary to high-throughput sequencing and discusses quality control issues, which are common among all NGS datasets. Next, we will present the alignment step and how it differs between the two analysis workflows. Finally, we focus on dataset specific downstream analysis, including peak calling and motif analysis for ChIP-seq and quantification of expression, transcriptome assembly and differential expression analysis for RNA-seq.
The timetable for this event can be found here.
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