72 materials found

Content provider: Bioinformatics Visualization 


Introducing Bivi.co

Introduction to the Bivi community presented as the introductory talk at the 1st Bivi Annual Meeting. Created at: 1st BiVi Annual Meeting.

Scientific topics: Phylogenetics, Pathway or network

Keywords: Anatomy Physiology and Atlases, Cells and Organisms, Genome, Molecular, Pathway, Phylogenetics, Populations

Resource type: Video

Introducing Bivi.co https://tess.elixir-europe.org/materials/introducing-bivi-co Introduction to the Bivi community presented as the introductory talk at the 1st Bivi Annual Meeting. Created at: 1st BiVi Annual Meeting. Prof. Jessie Kennedy Phylogenetics Pathway or network Anatomy Physiology and Atlases, Cells and Organisms, Genome, Molecular, Pathway, Phylogenetics, Populations 2016-12-08
Jalview

Dr James Proctor of the University of Dundee discusses the Jalview tool in the Vis Tools & Bio Challenges session of the 1st BiVi Annual Meeting Created at: 1st BiVi Annual Meeting.

Scientific topics: Phylogenetics

Keywords: Genome, Molecular, Phylogenetics

Resource type: Poster, Video

Jalview https://tess.elixir-europe.org/materials/jalview Dr James Proctor of the University of Dundee discusses the Jalview tool in the Vis Tools & Bio Challenges session of the 1st BiVi Annual Meeting Created at: 1st BiVi Annual Meeting. Dr. Jim Procter Jalview Coordinator Phylogenetics Genome, Molecular, Phylogenetics 2016-12-08
Validation and Visualisation of Electron Microscopy Density Fits

Poster presentation by Dr Maya Topf at 1st BiVi Annual Meeting in 2014 Created at: 1st BiVi Annual Meeting.

Resource type: Poster

Validation and Visualisation of Electron Microscopy Density Fits https://tess.elixir-europe.org/materials/validation-and-visualisation-of-electron-microscopy-density-fits Poster presentation by Dr Maya Topf at 1st BiVi Annual Meeting in 2014 Created at: 1st BiVi Annual Meeting. Prof. Maya Topf 2016-12-08
BiVi Genomes theme

Prof David Burt introduces the Genomes theme in bio-visualisation. Created at: 1st BiVi Annual Meeting.

Scientific topics: Phylogenetics

Keywords: Genome, Phylogenetics

Resource type: Video

BiVi Genomes theme https://tess.elixir-europe.org/materials/bivi-genomes-theme Prof David Burt introduces the Genomes theme in bio-visualisation. Created at: 1st BiVi Annual Meeting. Prof. David Willaim Burt PhD BSc FSB Phylogenetics Genome, Phylogenetics 2016-12-08
Visualization of Epigenetic Data

Esther Rheinbay (http://csb.mgh.harvard.edu/bradley_bernstein) talks about the current challenges of visualizing rapidly expanding epigenetic data and showcases some of the different tools which are available to researchers as part of the Integrative Genome Viewer...

Keywords: Genome, Molecular

Resource type: Video

Visualization of Epigenetic Data https://tess.elixir-europe.org/materials/visualization-of-epigenetic-data Esther Rheinbay (http://csb.mgh.harvard.edu/bradley_bernstein) talks about the current challenges of visualizing rapidly expanding epigenetic data and showcases some of the different tools which are available to researchers as part of the Integrative Genome Viewer (http://www.broadinstitute.org/igv/), the Roadmap Epigenomics Project (http://www.roadmapepigenomics.org/) and ECODE project (http://genome.ucsc.edu/encode/). This talk was presented at VIZBI 2013, an international conference series on visualizing biological data (http://vizbi.org). This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Genome, Molecular 2017-01-31
Keynote on Visual Analytics & Human/Computer Interfaces

Sara Irina Fabrikant discusses how analysis of scientific data can be enhanced using information visualization, visual analytics, cognition principles, and graphical user interface design. She focuses on the use of evaluation to valid visualization approaches. This talk was presented at VIZBI...

Keywords: Information visualisation

Resource type: Video

Keynote on Visual Analytics & Human/Computer Interfaces https://tess.elixir-europe.org/materials/keynote-on-visual-analytics-amp-human-computer-interfaces Sara Irina Fabrikant discusses how analysis of scientific data can be enhanced using information visualization, visual analytics, cognition principles, and graphical user interface design. She focuses on the use of evaluation to valid visualization approaches. This talk was presented at VIZBI 2013, an international conference series on visualizing biological data (http://vizbi.org). This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Information visualisation 2017-01-31
Epigenome Browser

Ting Wang (http://wang.wustl.edu/) addresses many of the challenges of navigating the complexity of genomic data and showcases the WashU Epigenome Browser that he is developing. The browser aims to give investigators maximum flexibility to explore and navigate the available genomic data in...

Keywords: Genome, Molecular

Resource type: Slideshow, Video

Epigenome Browser https://tess.elixir-europe.org/materials/epigenome-browser Ting Wang (http://wang.wustl.edu/) addresses many of the challenges of navigating the complexity of genomic data and showcases the WashU Epigenome Browser that he is developing. The browser aims to give investigators maximum flexibility to explore and navigate the available genomic data in multiple tracks, upload their own data as well as explore long-range interactions. He also highlights the need for the genome browsers to act as community resources and bioinformatics engines with the ability to analyse the mined data. This talk was presented at VIZBI 2013, an international conference series on visualizing biological data (http://vizbi.org). This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Dr. Ting Wang Genome, Molecular 2017-01-31
Browsing 3D Genomes

Jim Zheng (http://www.uthouston.edu/sbmi/faculty-and-staff/jim-zheng.htm) talks about the importance of understanding the 3D structure of genome and gives an introduction into Genome3D framework (http://www.genome3d.org/) which allows integration of multi-scale structural and genomic data within...

Keywords: Genome, Molecular

Resource type: Video

Browsing 3D Genomes https://tess.elixir-europe.org/materials/browsing-3d-genomes Jim Zheng (http://www.uthouston.edu/sbmi/faculty-and-staff/jim-zheng.htm) talks about the importance of understanding the 3D structure of genome and gives an introduction into Genome3D framework (http://www.genome3d.org/) which allows integration of multi-scale structural and genomic data within 3D. This talk was presented at VIZBI 2013, an international conference series on visualizing biological data (http://vizbi.org). This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Genome, Molecular 2017-01-31
enRoute: BioVis Best Paper

Alexander Lex (http://alexander-lex.com/) talks about enRoute, a tool for dynamic path extraction from biological pathway maps for in-depth experimental data analysis. He highlights the inherent problem with correlating real-life heterogeneous data to pathways, namely that they rarely follow the...

Scientific topics: Pathway or network

Keywords: Pathway

Resource type: Video

enRoute: BioVis Best Paper https://tess.elixir-europe.org/materials/enroute-biovis-best-paper Alexander Lex (http://alexander-lex.com/) talks about enRoute, a tool for dynamic path extraction from biological pathway maps for in-depth experimental data analysis. He highlights the inherent problem with correlating real-life heterogeneous data to pathways, namely that they rarely follow the same topography. enRoute tool allows the investigator to select defined sections of the pathway and display them in a linear fashion to allow for easier navigation of collated data. This talk was presented at VIZBI 2013, an international conference series on visualizing biological data (http://vizbi.org). This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Dr. Alexander Lex Pathway or network Pathway 2017-01-31
Visualizing RNA Structures and Alignments

Paul Gardner (http://www.biol.canterbury.ac.nz/people/gardner.shtml) talks about the challenges of visualization of RNA Structures and Alignments in the context of Rfam database (http://rfam.sanger.ac.uk/), a collection of non-coding RNAs, structured cis-regulatory elements and self-splicing...

Keywords: Genome, Molecular

Resource type: Video

Visualizing RNA Structures and Alignments https://tess.elixir-europe.org/materials/visualizing-rna-structures-and-alignments Paul Gardner (http://www.biol.canterbury.ac.nz/people/gardner.shtml) talks about the challenges of visualization of RNA Structures and Alignments in the context of Rfam database (http://rfam.sanger.ac.uk/), a collection of non-coding RNAs, structured cis-regulatory elements and self-splicing RNAs. This talk was presented at VIZBI 2013, an international conference series on visualizing biological data (http://vizbi.org). This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Genome, Molecular 2017-01-31
Non-coding RNA

Gisela Storz (http://irp.nih.gov/pi/gisela-storz) talks about the reliability and co-relation of anti-sense RNA sequence data. Comparison of RNA seq data across different experiments and from various sources reveals some discrepancies and lack of overlap. While the majority of RNA seq data is...

Keywords: Genome, Molecular

Resource type: Video

Non-coding RNA https://tess.elixir-europe.org/materials/non-coding-rna Gisela Storz (http://irp.nih.gov/pi/gisela-storz) talks about the reliability and co-relation of anti-sense RNA sequence data. Comparison of RNA seq data across different experiments and from various sources reveals some discrepancies and lack of overlap. While the majority of RNA seq data is robust and reliable, low abundance transcripts can be difficult to account for and the variations in experimental as well as computational conditions can lead to vastly different data.This talk was presented at VIZBI 2013, an international conference series on visualizing biological data (http://vizbi.org). This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Genome, Molecular 2017-01-31
Visualizing Protein Structures and Features

Sean O'Donoghue (http://odonoghuelab.org) talks about molecular spatial data and the emerging issue of sequence-structure gap mainly due to experimental difficulties in obtaining reliable (or any) structure information for a given protein. In his talk, he also gives a brief demonstration of...

Keywords: Molecular

Resource type: Video

Visualizing Protein Structures and Features https://tess.elixir-europe.org/materials/visualizing-protein-structures-and-features Sean O'Donoghue (http://odonoghuelab.org) talks about molecular spatial data and the emerging issue of sequence-structure gap mainly due to experimental difficulties in obtaining reliable (or any) structure information for a given protein. In his talk, he also gives a brief demonstration of Aquaria (http://aquaria.ws), a web-based 3D molecular structure visualization tool that combines data from various databases and allows investigators to easily view and explore proteins and protein-protein interactions.This talk was presented at VIZBI 2013, an international conference series on visualizing biological data (http://vizbi.org). This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Dr. Sean I O’Donoghue Molecular 2017-01-31
RNA-Seq

Cole Trapnell (http://www.broadinstitute.org/~cole/) focuses on isoform-resolution analysis of RNA -seq. RNA-seq is an assay used to determine the abundance of RNA transcripts in a transcriptome, the results of which can be used for gene discovery and expression analysis. In his talk Cole...

Keywords: Genome

Resource type: Video

RNA-Seq https://tess.elixir-europe.org/materials/rna-seq Cole Trapnell (http://www.broadinstitute.org/~cole/) focuses on isoform-resolution analysis of RNA -seq. RNA-seq is an assay used to determine the abundance of RNA transcripts in a transcriptome, the results of which can be used for gene discovery and expression analysis. In his talk Cole highlights the inherent challenges associated with the analysis of isoform-resolution data and proposes several different areas that are open for improvement.This talk was presented at VIZBI 2013, an international conference series on visualizing biological data (http://vizbi.org). This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Genome 2017-01-31
Comparison and assemblies

Thomas Ferrin (http://www.cgl.ucsf.edu/home/tef/) talks about visualization tools for interactive modeling of biological structures with an emphasis on Chimera (http://www.cgl.ucsf.edu/chimera/), an extensible molecular modeling software.This talk was presented at VIZBI 2013, an international...

Keywords: Molecular

Resource type: Video

Comparison and assemblies https://tess.elixir-europe.org/materials/comparison-and-assemblies Thomas Ferrin (http://www.cgl.ucsf.edu/home/tef/) talks about visualization tools for interactive modeling of biological structures with an emphasis on Chimera (http://www.cgl.ucsf.edu/chimera/), an extensible molecular modeling software.This talk was presented at VIZBI 2013, an international conference series on visualizing biological data (http://vizbi.org). This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Prof. Thomas Ferrin Molecular 2017-01-31
Visualizing odors with insect brains

Martin Strauch presents a brief overview of his BioVis 2012 Honorable Mention paper on visualising odors with insect brains.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013.

Keywords: Anatomy Physiology and Atlases

Resource type: Video

Visualizing odors with insect brains https://tess.elixir-europe.org/materials/visualizing-odors-with-insect-brains Martin Strauch presents a brief overview of his BioVis 2012 Honorable Mention paper on visualising odors with insect brains.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Martin Strauch Anatomy Physiology and Atlases 2017-01-31
Evolution of Protein Structure and Function

Ian Sillitoe (http://orengogroup.info/) talks about the protein structure classification with particular emphasis on superfamily-centric taxonomy which is used in the CATH (http://www.cathdb.info/) database.This video was filmed and distributed with permission under a Creative Commons...

Keywords: Molecular

Resource type: Video

Evolution of Protein Structure and Function https://tess.elixir-europe.org/materials/evolution-of-protein-structure-and-function Ian Sillitoe (http://orengogroup.info/) talks about the protein structure classification with particular emphasis on superfamily-centric taxonomy which is used in the CATH (http://www.cathdb.info/) database.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Molecular 2017-01-31
Data visualization in proteomics

Martin Miller talks about visualization of proteome data with particular emphasis on modeling phosphorylation sites. He gives a brief introduction into different methods for the analysis of the single cell proteome from antibody-based methods to mass spectrometry and fluorescence tagging. With...

Keywords: Molecular

Resource type: Video

Data visualization in proteomics https://tess.elixir-europe.org/materials/data-visualization-in-proteomics Martin Miller talks about visualization of proteome data with particular emphasis on modeling phosphorylation sites. He gives a brief introduction into different methods for the analysis of the single cell proteome from antibody-based methods to mass spectrometry and fluorescence tagging. With the onset of targeted and shot-gun mass spectrometry, there is an increasing amount of proteomics data which will need to be classified, integrated and available for analysis in a meaningful and accessible manner.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Molecular 2017-01-31
Biological Networks

Scooter Morris (http://www.cgl.ucsf.edu/home/scooter/) talks about the opportunities and challenges in mapping biological networks and gives an brief overview of Cytoscape (http://www.cytoscape.org/), an open source bioinformatics software platform for visualizing molecular interaction...

Scientific topics: Pathway or network

Keywords: Cells and Organisms, Pathway

Resource type: Video

Biological Networks https://tess.elixir-europe.org/materials/biological-networks Scooter Morris (http://www.cgl.ucsf.edu/home/scooter/) talks about the opportunities and challenges in mapping biological networks and gives an brief overview of Cytoscape (http://www.cytoscape.org/), an open source bioinformatics software platform for visualizing molecular interaction networks.(PLEASE NOTE: There is a small formatting problem with the presentation slides for the first 2 min of the talk only). This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Dr. Scooter Morris Pathway or network Cells and Organisms, Pathway 2017-01-31
Keynote on Visual Design Principles

Martin Krzywinski (http://mkweb.bcgsc.ca/) gives a great primer on the key principles of effective visual design in biological sciences. During the talk he highlights the principles with examples from various peer-reviewed publications and discusses the practical aspects of effective figure...

Keywords: Genome, HCI, Information visualisation, Communication

Resource type: Video

Keynote on Visual Design Principles https://tess.elixir-europe.org/materials/keynote-on-visual-design-principles Martin Krzywinski (http://mkweb.bcgsc.ca/) gives a great primer on the key principles of effective visual design in biological sciences. During the talk he highlights the principles with examples from various peer-reviewed publications and discusses the practical aspects of effective figure design.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Martin Krzywinski Genome, HCI, Information visualisation, Communication 2017-01-31
Cellular Image Data

Peter Sorger (http://sorger.med.harvard.edu/) and Bang Wong (http://bang.clearscience.info/) talk about the challenges of visualising large sets of multidimensional data (e.g. single cell imaging, dose-response assays, multiplex biochemistry etc.).This video was filmed and distributed with...

Keywords: Cells and Organisms

Resource type: Video

Cellular Image Data https://tess.elixir-europe.org/materials/cellular-image-data Peter Sorger (http://sorger.med.harvard.edu/) and Bang Wong (http://bang.clearscience.info/) talk about the challenges of visualising large sets of multidimensional data (e.g. single cell imaging, dose-response assays, multiplex biochemistry etc.).This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Bang Wong Cells and Organisms 2017-01-31
Keynote on Communicating Science Visually

Felice Frankel delivers her keynote address in which she talks about how to effectively use visual tools to communicate science.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013.

Keywords: Communication

Resource type: Video

Keynote on Communicating Science Visually https://tess.elixir-europe.org/materials/keynote-on-communicating-science-visually Felice Frankel delivers her keynote address in which she talks about how to effectively use visual tools to communicate science.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Communication 2017-01-31
Developmental Anatomy

Sean Megason (http://megason.med.harvard.edu/) talks about the challenges and caveats of multiscale imaging, visualisation and analysis and highlights some of the tools that are used for processing and analysis of in toto imaging such as GoFigure2 (http://www.gofigure2.org/) and ACME...

Keywords: Anatomy Physiology and Atlases

Resource type: Video

Developmental Anatomy https://tess.elixir-europe.org/materials/developmental-anatomy Sean Megason (http://megason.med.harvard.edu/) talks about the challenges and caveats of multiscale imaging, visualisation and analysis and highlights some of the tools that are used for processing and analysis of in toto imaging such as GoFigure2 (http://www.gofigure2.org/) and ACME (https://wiki.med.harvard.edu/SysBio/Megason/ACME).  This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Anatomy Physiology and Atlases 2017-01-31
Physiology & Function

Daniel Haehn talks about visualising medical and scientific data (such as MRI and CT scans, microscopy scan stacks etc.) in 3D and introduces some of the open-source tools available.This video was filmed and distributed with permission under a Creative Common license. Created at: VIZBI 2013.

Keywords: Anatomy Physiology and Atlases

Resource type: Video

Physiology & Function https://tess.elixir-europe.org/materials/physiology-amp-function Daniel Haehn talks about visualising medical and scientific data (such as MRI and CT scans, microscopy scan stacks etc.) in 3D and introduces some of the open-source tools available.This video was filmed and distributed with permission under a Creative Common license. Created at: VIZBI 2013. Anatomy Physiology and Atlases 2017-01-31
Genes into Geometry

Mark Henkelman gives an introduction on the use of 3D imaging in neuroanatomy to explore consequences of genetic alterations on anatomical structures and infer function.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013.

Keywords: Anatomy Physiology and Atlases

Resource type: Video

Genes into Geometry https://tess.elixir-europe.org/materials/genes-into-geometry Mark Henkelman gives an introduction on the use of 3D imaging in neuroanatomy to explore consequences of genetic alterations on anatomical structures and infer function.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Anatomy Physiology and Atlases 2017-01-31
Infectious Disease Host/Pathogen Relationships

Bruno Sobral demonstrates how collaboration with the bench scientists to learn about their needs, uses and challenges can lead to design of more effective resources for data exploration and analysis. He emphasises the importance of designing in a user-centric manner and highlights the importance...

Keywords: Populations, HCI

Resource type: Video

Infectious Disease Host/Pathogen Relationships https://tess.elixir-europe.org/materials/infectious-disease-host-pathogen-relationships Bruno Sobral demonstrates how collaboration with the bench scientists to learn about their needs, uses and challenges can lead to design of more effective resources for data exploration and analysis. He emphasises the importance of designing in a user-centric manner and highlights the importance of human-computer interface professionals in the design process.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Populations, HCI 2017-01-31
Metagenomics

Susannah Tringe talks about metagenomics analysis tools and the challenges of gaining a systems-level understanding of a microbial community. She notes that there are some good analysis tools available for analysis at the 16S sequence and unassembled metagenome level, but more effort into...

Scientific topics: Phylogenetics

Keywords: Genome, Phylogenetics, Populations

Resource type: Video

Metagenomics https://tess.elixir-europe.org/materials/metagenomics-6625dde2-a7da-4cc2-a51d-14c1c31b51b2 Susannah Tringe talks about metagenomics analysis tools and the challenges of gaining a systems-level understanding of a microbial community. She notes that there are some good analysis tools available for analysis at the 16S sequence and unassembled metagenome level, but more effort into analysis and visualisation solutions are required at the microbial community and ecosystem level.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Phylogenetics Genome, Phylogenetics, Populations 2017-01-31
Making Science Beautiful

In this presentation David Goodsell reveals the beauty of science through his scientific and artistic endeavors. He explores the visual techniques which have been used to communicate the detail of the molecular biology world to different audiences.David Goodsell (http://bit.ly/DGoodsell) is an...

Keywords: Molecular, Communication

Resource type: Video

Making Science Beautiful https://tess.elixir-europe.org/materials/making-science-beautiful In this presentation David Goodsell reveals the beauty of science through his scientific and artistic endeavors. He explores the visual techniques which have been used to communicate the detail of the molecular biology world to different audiences.David Goodsell (http://bit.ly/DGoodsell) is an Associate Professor of Molecular Biology at the Scripps Institute, California Campus. Created at: VIZBI 2014. Molecular, Communication 2017-01-31
Geographic & Evolutionary Visualization

Daniel Janies showcases SupraMap, a web-based app that integrates genetic sequences of pathogens with geographic data points to map the spread of diseases and track mutations.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013.

Scientific topics: Phylogenetics

Keywords: Phylogenetics, Populations

Resource type: Video

Geographic & Evolutionary Visualization https://tess.elixir-europe.org/materials/geographic-amp-evolutionary-visualization Daniel Janies showcases SupraMap, a web-based app that integrates genetic sequences of pathogens with geographic data points to map the spread of diseases and track mutations.This video was filmed and distributed with permission under a Creative Commons license. Created at: VIZBI 2013. Phylogenetics Phylogenetics, Populations 2017-01-31
BiVi theme: Anatomy, Physiology and Atlases

Prof Richard Baldock discusses bio-visualisation within the Anatomy, Physiology and Atlases theme Created at: 1st BiVi Annual Meeting.

Keywords: Anatomy Physiology and Atlases

Resource type: Video

BiVi theme: Anatomy, Physiology and Atlases https://tess.elixir-europe.org/materials/bivi-theme-anatomy-physiology-and-atlases Prof Richard Baldock discusses bio-visualisation within the Anatomy, Physiology and Atlases theme Created at: 1st BiVi Annual Meeting. Prof. Richard Baldock Anatomy Physiology and Atlases 2017-02-01
Foldsynth

Prof. Frederic Fol Leymarie discusses the Foldsynth project at 1st BiVi in 2014. A poster presented at the meeting is also included. Created at: 1st BiVi Annual Meeting.

Keywords: Molecular

Resource type: Poster, Video

Foldsynth https://tess.elixir-europe.org/materials/foldsynth Prof. Frederic Fol Leymarie discusses the Foldsynth project at 1st BiVi in 2014. A poster presented at the meeting is also included. Created at: 1st BiVi Annual Meeting. Prof. Frederic Fol Leymarie Ph.D. Molecular 2017-02-01