Transcriptomics - Differential abundance testing of small RNAs

Training material for all kinds of transcriptomics analysis.

Questions of the tutorial:

  • What small RNAs are expressed?
  • What RNA features have significantly different numbers of small RNAs targeting them between two conditions?

Objectives of the tutorial:

  • Process small RNA-seq datasets to determine quality and reproducibility.
  • Filter out contaminants (e.g. rRNA reads) in small RNA-seq datasets.
  • Differentiate between subclasses of small RNAs based on their characteristics.
  • Identify differently abundant small RNAs and their targets.

Resource type: Tutorial

Authors: malloryfreeberg

External resources:
Transcriptomics - Differential abundance testing of small RNAs https://tess.elixir-europe.org/materials/transcriptomics-differential-abundance-testing-of-small-rnas Training material for all kinds of transcriptomics analysis. Questions of the tutorial: - What small RNAs are expressed? - What RNA features have significantly different numbers of small RNAs targeting them between two conditions? Objectives of the tutorial: - Process small RNA-seq datasets to determine quality and reproducibility. - Filter out contaminants (e.g. rRNA reads) in small RNA-seq datasets. - Differentiate between subclasses of small RNAs based on their characteristics. - Identify differently abundant small RNAs and their targets.