Transcriptomics - De novo transcriptome reconstruction with RNA-Seq

Training material for all kinds of transcriptomics analysis.

Questions of the tutorial:

  • What genes are differentially expressed between G1E cells and megakaryocytes?
  • How can we generate a transcriptome de novo from RNA sequencing data?

Objectives of the tutorial:

  • Analysis of RNA sequencing data using a reference genome
  • Reconstruction of transcripts without reference transcriptome (de novo)
  • Analysis of differentially expressed genes

Resource type: Tutorial

Authors: malloryfreeberg, moheydarian

External resources:
Transcriptomics - De novo transcriptome reconstruction with RNA-Seq https://tess.elixir-europe.org/materials/transcriptomics-de-novo-transcriptome-reconstruction-with-rna-seq Training material for all kinds of transcriptomics analysis. Questions of the tutorial: - What genes are differentially expressed between G1E cells and megakaryocytes? - How can we generate a transcriptome de novo from RNA sequencing data? Objectives of the tutorial: - Analysis of RNA sequencing data using a reference genome - Reconstruction of transcripts without reference transcriptome (de novo) - Analysis of differentially expressed genes