Statistics and RNA-Seq

Statistics and RNA-Seq

Keywords

FASTQ, Alignment, BAM, Differential-expression, Statistical-model, Exploratory-analysis

Authors

  • Benilton Carvalho @benilton

Top | Keywords | Authors | Description | Aims | Prerequisites | Target audience | Learning objectives | Materials | Data | Technical requirements | Literature references

Description

This lecture and optional practical introduce the relevant statistical concepts along with its computational counterparts used on a common RNA-Seq data analysis workflow.

Aims

Make the participants aware of steps involved in the analysis of RNA-Seq data and comfortable with the statistical concepts used during such analyses, in a way that the attendees are able to critically question results and understand the rationale of statistical procedures used in high-throughput data analyses.

Top | Keywords | Authors | Description | Aims | Prerequisites | Target audience | Learning objectives | Materials | Data | Technical requirements | Literature references

Prerequisites

  • None

Target audience

beginner, biologists, statisticians, mathematicians, computer scientists

Learning objectives

  • Make the attendee comfortable with the statistical nomenclature used in RNA-Seq data analysis;
  • Clarify misconceptions in the use of statistical models;
  • Elaborate the interpretation of parameters in statistical models;
  • Stimulate critical thinking and interpretation of high-throughput data modelling;
  • Conduct basic analysis of RNA-Seq data.

Top | Keywords | Authors | Description | Aims | Prerequisites | Target audience | Learning objectives | Materials | Data | Technical requirements | Literature references

Materials

  • Slides in PPTX
  • Slides in PDF

Data

### Description
Count table for pilot study on rats comparing pilocarpine-induced animals to controls. The objective is to identify a list of genes that are candidates for differential expression when comparing the treated group to the controls.

Availability

exampleRNASeq.txt

Top | Keywords | Authors | Description | Aims | Prerequisites | Target audience | Learning objectives | Materials | Data | Technical requirements | Literature references

Technical requirements

Requirements described on Tutorial

Literature references

  • todo

Top | Keywords | Authors | Description | Aims | Prerequisites | Target audience | Learning objectives | Materials | Data | Technical requirements | Literature references

Keywords: FASTQ, Alignment, BAM, Differential-expression, Statistical-model, Exploratory-analysis

Authors: Benilton Carvalho @benilton

Statistics and RNA-Seq https://tess.elixir-europe.org/materials/statistics-and-rna-seq This lecture and optional practical introduce the relevant statistical concepts along with its computational counterparts used on a common RNA-Seq data analysis workflow. FASTQ, Alignment, BAM, Differential-expression, Statistical-model, Exploratory-analysis