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3 events found

Scientific topics: Ontology and terminology  or RNA-Seq 

  • WORKSHOP: Single cell RNAseq analysis in R

    22 - 23 August 2022

    WORKSHOP: Single cell RNAseq analysis in R https://tess.elixir-europe.org/events/workshop-single-cell-rnaseq-analysis-in-r Analysis and interpretation of single cell RNAseq (scRNAseq) data requires dedicated workflows. In this hands-on workshop we will show you how to perform single cell analysis using[ Seurat](https://satijalab.org/seurat/index.html) - an R package for QC, analysis, and exploration of single-cell RNAseq data. We will discuss the ‘why’ behind each step and cover reading in the count data, quality control, filtering, normalisation, clustering, UMAP layout and identification of cluster markers. We will also explore various ways of visualising single cell expression data. **Lead trainers:** Dr Sarah Williams, Senior Bioinformatician, QCIF Mr Nick Matigan, Biostatistician and Bioinformatician, QCIF Dr Ahmed Mehdi, Senior Biostatistician, QCIF **Format** This online workshop will take place over two half-day sessions. You must attend both sessions. Expert trainers will guide you through each of the topics and provide activities to help you put your new skills into action. **Date/Time:** 22 and 23 August 2022, 1-5pm AEST/ ACST/AWST **Location:** Online **Learning outcomes:** By the end of the workshop you should be able to: 1. Load gene counts into a Seurat format 2. Perform QC and select cells for further analysis 3. Filter and normalise scRNAseq data 4. Cluster cells and identify cluster markers 5. Visualise scRNAseq expression data **Who the workshop is for:** This workshop is for Australian researchers who have or will work on scRNAseq data as part of their projects. You must be associated with an Australian organisation for your application to be considered. This workshop will not teach you the basics of R. While you don’t need to be an expert, you require some basic familiarity with R. For example you should be able to work with tables of data, know how to load an R package, make basic plots (ideally with ggplot2). You should also have basic knowledge of single cell RNA sequencing technology. **How to apply:** This workshop is free but participation is subject to application with selection. Applications will be reviewed by the organising committee and all applicants will be informed of the status of their application (successful, waiting list, unsuccessful). Successful applicants will be provided with a Zoom meeting link closer to the date. Applications close at **11:59pm AEDT, Wednesday 10 August 2022**. **[Apply here](https://biocommons-scrnaseq.eventbrite.com.au/)** _This workshop is presented by the[ Australian BioCommons](https://www.biocommons.org.au/) and[ Queensland Cyber Infrastructure Foundation (QCIF)](https://www.qcif.edu.au/) with the assistance of a network of facilitators from the national[ Bioinformatics Training Cooperative](https://www.biocommons.org.au/training-cooperative)._ _This event is part of a series of[ bioinformatics training events](https://www.biocommons.org.au/events). If you'd like to hear when registrations open for other events, please[ subscribe](https://www.biocommons.org.au/subscribe) to Australian BioCommons_ 2022-08-22 13:00:00 UTC 2022-08-23 17:00:00 UTC Australian BioCommons RNA-Seq Australian Biocommons training@biocommons.org.au [] [] 80 workshops_and_courses registration_of_interest Single Cell technologiesscRNAseqSeuratR software
  • WORKSHOP: RNA-Seq: reads to differential genes and pathways

    27 September 2022

    WORKSHOP: RNA-Seq: reads to differential genes and pathways https://tess.elixir-europe.org/events/workshop-rna-seq-reads-to-differential-genes-and-pathways RNA sequencing (RNA-seq) is a common method used to understand the differences in gene expression and molecular pathways between two or more groups. This workshop introduces the fundamental concepts of RNA sequencing experiments and will allow you to try out the analysis using data from a study of Williams-Beuren Syndrome, a rare disease. In the first part of the workshop you will learn how to convert sequence reads into analysis ready count data. To do this we will use[ nf-core/rnaseq](https://nf-co.re/rnaseq/usage) - a portable, scalable, reproducible and publicly available workflow on[ Pawsey Nimbus Cloud](https://pawsey.org.au/systems/nimbus-cloud-service/). In the second part of the workshop you will use the count data you created to identify differential genes and pathways using R/Rstudio. By the end of the workshop, you should be able to perform your own RNA-seq analysis for differential gene expression and pathway analysis! **Lead Trainers:** Dr Nandan Deshpande, Senior Research Bioinformatician, Sydney Informatics Hub **Date/Time**: 27 & 28 September 2022, 2-5pm AEST/1:30-4:30pm ACST/12-3pm AWST **Format:** This online workshop will take place over two three-hour sessions. You must attend both sessions in order to get the most out of the workshop. Expert trainers will introduce new topics and guide you through hands-on activities to help you put your new skills into action. **Learning outcomes:** By the end of the workshop you should be able to: 1. List the steps involved in analysis of RNA-seq data 2. Describe key concepts and considerations for RNA-seq experiments 3. Describe the benefits of using nf-core workflows 4. Deploy an RNA-seq nf-core workflow on Pawsey’s Nimbus Cloud to perform: 1. Quality control 2. Alignment 3. Quantification to generate raw counts 5. Use R/RStudio on Pawsey’s Nimbus Cloud to perform 4. Quality control 5. Identify differentially expressed genes using DESeq2 6. Perform functional enrichment/pathway analysis **Who the workshop is for:** This workshop is for Australian researchers who have or will work on RNAseq data as part of their projects. You must be associated with an Australian organisation for your application to be considered. The workshop will be conducted in a Unix environment and will use R/RStudio. Basic command line knowledge is required. You must know how to navigate the directory structure and copy files between the computers. If you need a refresher on Unix/Linux try[ this online tutorial](https://linuxjourney.com/lesson/the-shell). Basic knowledge of R/RStudio is required. You must know how to set up directories, run commands, reading in and outputting files. If you need a refresher on R/RStudio try the[ Introduction to R and RStudio section](https://swcarpentry.github.io/r-novice-gapminder/01-rstudio-intro/index.html) of this online tutorial. It’s recommended that you watch the webinar[ Portable, reproducible and scalable bioinformatics workflows using Nextflow and Pawsey Nimbus Cloud](http://biocommons.org.au/events/nextflow-nimbus) in advance. You may join this webinar live on 20 September 2022. A recording will be posted on the[ Australian BioCommons YouTube Channel](https://www.youtube.com/c/AustralianBioCommonsChannel). **How to apply:** This workshop is free but participation is subject to application with selection. Applications close at **11:59pm AEST, Sunday 11 September 2022**. Applications will be reviewed by the organising committee and all applicants will be informed of the status of their application (successful, waiting list, unsuccessful). Successful applicants will be provided with a Zoom meeting link closer to the date. **[Apply here](https://biocommons-rnaseq-2022.eventbrite.com.au/)** _This workshop is presented by the[ Australian BioCommons](https://www.biocommons.org.au/), Sydney Informatics Hub and Pawsey Supercomputing Research Centre with the assistance of a network of facilitators from the national[ Bioinformatics Training Cooperative](https://www.biocommons.org.au/training-cooperative)._ _This event is part of a series of[ bioinformatics training events](https://www.biocommons.org.au/events). If you'd like to hear when registrations open for other events, please[ subscribe](https://www.biocommons.org.au/subscribe) to Australian BioCommons_ 2022-09-27 14:00:00 UTC 2022-09-27 17:00:00 UTC Australian BioCommons RNA-Seq Workflows Gene expression Functional genomics Bioinformatics Australian Biocommons training@biocommons.org.au [] [] 100 workshops_and_courses registration_of_interest BioinformaticsRNA-seqGene ExpressionPathway analysisNextflownf-coreWorkflowsFunctional analysis
  • scRNA-seq Workshop

    28 - 30 September 2022

    Torino, Italy

    scRNA-seq Workshop https://tess.elixir-europe.org/events/scrna-seq-workshop This course aims to provide participants with theoretical and practical knowledge on how to perform bioinformatic analyses of scRNA-seq data using open-source software solutions. scRNA-seq analyses will be performed using the tools available as part of the Reproducible Bioinformatics Project (rCASC). This course is suitable for biologists who are new to single cell gene expression technology. Knowledge of statistics as well as computing skills are not necessary prior to attending the course. 2022-09-28 09:00:00 UTC 2022-09-30 17:00:00 UTC B&Gu, University of Turin, EuroClone Via Nizza, 52, Torino, Italy Via Nizza, 52 Torino Città Metropolitana di Torino Italy 10126 Sequencing RNA-Seq University of Turin, Italy raffaele.calogero@unito.it [] biologistsbioinformaticians 20 workshops_and_courses first_come_first_served scRNA-seqNext generation sequencing data analysis

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