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Sponsors: The Plant Cell  or H3ABioNet 

  • H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 1

    20 August 2018

    H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 1 https://tess.elixir-europe.org/events/h3abionet-2018-genotyping-chip-data-analysis-and-gwas-lecture-series #### Computational requirements for running the H3ABioNet GWAS workflows The first of a series of seven online lectures for Genome Wide Association Studies (GWAS) will cover the technical requirements for setting up a your computational environment for running the H3ABioNet GWAS workflows. In this inaugural lecture of the series, Prof. Hazelhurst will cover the the following topics: Installing and using Nextflow Installing and using Github Use of containers for packaging and running tools Pulling the GWAS pipeline from Github and running it As this lecture aims to provide attendees with an environment to the run the H3ABioNet GWAS workflow at their own pace, there are some preliminary software requirements: Either a Linux machine or an Apple running macOS Ideally you should have machine with at least 2-4 cores and 8GB of RAM. Java 8 Nextflow installed (see installation instructions at https://www.nextflow.io/) Python 3 Please also install either Docker OR the following dependencies using pip3: Pandas, Matplotlib, Openpyxl, SciPy, NumPy PLINK 1.9 [Please also refer to the following documentation to obtain the H3ABioNet GWAS workflow]: https://github.com/h3abionet/h3agwas/blob/master/README.md 2018-08-20 15:00:00 UTC 2018-08-20 16:30:00 UTC H3ABioNet Population genomics Genotyping experiment Computational biology Workflows GWAS study Bioinformatics H3ABioNet info@h3abionet.org H3ABioNet Anyone intersted in GWAS and using the H3Africa genotyping chipAnyone who wants to learn more about GWAS workshops_and_courses [] NextflowDockerH3ABioNetGWASWorkflowsGenotyping array analysis bioinformaticsAfricaPopulation GenomicsReproducible ScienceH3Africa genotyping arrayHigh performance computingCloud computingGWAS workflowH3ABioNet GWAS 2018 Lecture Series
  • H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 2

    22 August 2018

    H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 2 https://tess.elixir-europe.org/events/h3abionet-2018-genotyping-chip-data-analysis-and-gwas-lecture-series-4a0ce1cc-f48d-4f1e-98b5-2bfc55abce6a ### Overview of Genome Wide Association Studies and study designs The second of a series of seven online lectures for Genome Wide Association Studies (GWAS) will provide a basic overview on genome-wide association studies (GWAS). This is timely with the H3Africa genotyping data becoming available for several of the H3Africa research groups. To enable genomic research, the H3Africa Consortium had to build some of its own resources. This has involved sequencing whole genomes from selected under-represented African populations, analyzing the data to identify common genetic variation across major groups and developing a unique GWAS array, enriched for common African variation. The H3Africa SNP genotyping array, is currently the best-suited array available for African genome-wide association studies. What does it take to develop a genome study to understand genetic and environmental contributions to complex disease traits? This GWAS lecture will cover the research process from protocol development to data quality control and GWAS analysis approaches. It will include the two main study designs: Case : control (e.g. diabetes, hypertension, kidney disease) Continuous trait (e.g. LDL-Cholesterol, body mass index, height) GWAS. I will discuss the basic building blocks of a GWAS study, the different study designs, power of a study to detect genetic association (based in sample size, allele frequency and expected effect size), and replication studies. The advantages and disadvantages of doing GWAS in African populations will be discussed. 2018-08-22 15:00:00 UTC 2018-08-22 17:00:00 UTC H3ABioNet Genotyping experiment Population genetics Population genomics GWAS study H3ABioNet info@h3abionet.org H3ABioNet Anyone intersted in GWAS and using the H3Africa genotyping chip workshops_and_courses [] H3ABioNetGWASH3AfricaH3Africa genotyping arrayAfrican populationsStudy designAWI-GENAfrican genomicsPopulations GenomicsGenetic DiversityH3Africa genotyping arrayCase Control studyGenotypesComplex traitsGWAS study designsAllelic associationGenotype associationGWAS workflowQuantitative traitsSample sizeCommon and rare variantsAfrican genome structureH3ABioNet GWAS 2018 Lecture Series
  • H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 3

    27 August 2018

    H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 3 https://tess.elixir-europe.org/events/h3abionet-2018-genotyping-chip-data-analysis-and-gwas-lecture-series-lecture-3 ### Genotype calling from Illumina files The third of a series of seven H3ABioNet online lectures for Genome Wide Association Studies (GWAS) will introduce genotyping SNP array chips with particular emphasis on the H3Africa genotype chip. The lecture will cover common file formats when obtaining genotyping chip data from a service provider such as Illumina and software used for genotype calling. This lecture will also cover genotype calling, sample and probe quality control, probe viewing and exporting of data using GenomeStudio. Converting the data to PLINK format and some tips on troubleshooting and common pitfalls will be discussed. 2018-08-27 15:00:00 UTC 2018-08-27 16:00:00 UTC H3ABioNet Population genomics Bioinformatics Genotyping experiment Genomics Computational biology H3ABioNet info@h3abionet.org H3ABioNet Anyone intersted in GWAS and using the H3Africa genotyping chip 150 workshops_and_courses [] Genotype callingH3ABioNetGenotype callingGenome StudioH3Africa genotyping arrayAfrican populationsArray processingIllumina arraysFile formatsAnnotationsNon-polymorphicTop/Bottom annotationGenotype array probesIntensitiesConvert to PLINK format BioinformaticsGenome Wide Association Studies
  • H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 4

    29 August 2018

    H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 4 https://tess.elixir-europe.org/events/h3abionet-2018-genotyping-chip-data-analysis-and-gwas-lecture-series-lecture-4 ### Quality control and its importance in GWAS In the fourth of a series of seven H3ABioNet online lectures for Genome Wide Association Studies (GWAS), the importance of doing good quality control for a GWAS will be discussed. The lecture will cover the types of errors one should control for, the specific bioinformatics tools used for quality control. Specific sample and SNP quality control steps that should be undertaken in a GWAS will be explored using specific examples. 2018-08-29 15:00:00 UTC 2018-08-29 16:30:00 UTC H3ABioNet Genotyping experiment GWAS study Computational biology Bioinformatics Population genomics Population genetics H3ABioNet info@h3abionet.org H3ABioNet Anyone intersted in GWAS and using the H3Africa genotyping chip 150 workshops_and_courses [] Genome Wide Association StudiesGenotype callingH3Africa genotyping arrayAfrican populationsQuality Control for GWASSample Quality ControlSNP Quality ControlPopulation GenomicsGenotypingPLINK formatPrincipal Component AnalysisLow minor allele frequencyMissingnessGWAS workflowDiscordant Sex informationNextflowGenotyping rate callHeterozygosity rateRelated and duplicate individualsIdentity by DescentHardy Weinberg EquilibriumBioinformaticsH3ABioNet GWAS 2018 Lecture Series
  • H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 5

    3 September 2018

    H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 5 https://tess.elixir-europe.org/events/h3abionet-2018-genotyping-chip-data-analysis-and-gwas-lecture-series-ce96b48a-423a-435c-beae-9ddfcaba38a6 ### Population structure in GWAS The fifth of a series of seven H3ABioNet online lectures for Genome Wide Association Studies (GWAS) will introduce the concept of population structure/stratification (PS) and suggest why it is critical to consider PS in a GWAS. The lecture will begin by familiarizing the types of PS that are encountered and explain the factors that could cause them to appear in a GWAS dataset. It will then cover the methods that are commonly used to identify PS in a dataset and finally discuss the available computational approaches to correct for PS in a GWAS. Date: 3rd September 2018 Time: 2pm WAT / 3pm CAT / 4pm EAT [Url to join the lecture]: http://meeting.uct.ac.za/h3abionet_gwas/ 2018-09-03 15:00:00 UTC 2018-09-03 16:30:00 UTC H3ABioNet Genotyping experiment Bioinformatics GWAS study Population genetics Population genomics H3ABioNet info@h3abionet.org H3ABioNet Anyone intersted in GWAS and using the H3Africa genotyping chip 150 meetings_and_conferencesworkshops_and_courses first_come_first_served Population structureGWASCorrect for population structureH3ABioNetH3AfricaAWI-GEN
  • H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 6

    5 September 2018

    H3ABioNet 2018 Genotyping Chip Data Analysis and GWAS lecture series - Lecture 6 https://tess.elixir-europe.org/events/h3abionet-2018-genotyping-chip-data-analysis-and-gwas-lecture-series-lecture-6 ### Imputation and its importance in GWAS The sixth of a series of seven H3ABioNet online lectures for Genome Wide Association Studies (GWAS) will introduce the fundamentals of genotype imputation and its importance in GWAS. This lecture will cover the reference panels, file formats, algorithms and tools commonly used for imputation. It will discuss the assessment of the quality of imputation as well as the evaluation of reference panel and imputation methods. The lecture will also explain how imputation enhances the scope a GWAS and describe some of the additional analyses that become possible due to the use of imputed data. 2018-09-05 15:00:00 UTC 2018-09-05 16:30:00 UTC H3ABioNet Population genetics Population genomics Computational biology Bioinformatics Genotyping experiment GWAS study H3ABioNet info@h3abionet.org H3ABioNet Anyone intersted in GWAS and using the H3Africa genotyping chip 150 workshops_and_courses first_come_first_served ImputationGWASAfrican populationsImputation panel
  • Regulatory Oxylipins

    1 - 4 April 2019

    Gent, Belgium

    Elixir node event
    Regulatory Oxylipins https://tess.elixir-europe.org/events/regulatory-oxylipins-661da9dc-2159-4a34-8809-7851dfbe0b8a Oxylipins, from plants and other organisms, form a constantly growing group of signalling molecules that comprise oxygenated fatty acids and their metabolites. The most renowned regulatory oxylipins are undoubtedly the jasmonates, which, following their discovery in the 1960s, became recognized as regulators of defence, floral organ development and fertility, as growth inhibitors and modulators of senescence, and as elicitors of specialized metabolism in numerous plant species. As such, the jasmonates currently belong to an elite group of heavily investigated phytohormones. Over the last decade, the understanding of biosynthesis, metabolism, and action of regulatory oxylipins has greatly advanced. The ROXY2019 meeting wishes to bring together the international community and leading researchers in the field. Thereby it aims to provide a timely and exciting forum to present and discuss the recent advances and expertise on the study of these molecules that are so crucial for plant growth, development, metabolism, and interaction with the environment. There will be seven plenary sessions that, through combining lectures from invited speakers and short talks on selected abstracts and posters, will highlight the various aspects involved in Regulatory Oxylipin research. These sessions cover the following major themes: Regulatory oxylipins in emerging model systems and non-plant systems, Biochemistry and structural biology, Control of growth and defence, Control of Metabolism, Reproduction, Jasmonate signalling mechanisms and Long-distance signalling, Ecology. We look forward to your contributions to make this an exciting conference. See you in Gent! The organizers Alain Goossens and Ted Farmer Poster information Format: A0 (841 x 1189 mm / 33.1 x 46.8 in), portrait orientation 2019-04-01 09:00:00 UTC 2019-04-04 17:00:00 UTC VIB Conferences - Alain Goossens & Ted Farmer Ghent University - Aula, 9, Voldersstraat, Gent, Belgium Ghent University - Aula, 9, Voldersstraat Gent Oost-Vlaanderen Belgium 9000 [] conferences@vib.be Faculty of ScienceJournal of Experimental BotanyPCP (Plant & Cell Physiology)The Plant CellThe Plant Journal [] [] [] Regulatory OxylipinsBiochemistrystructural biologyMetabolismreproductionJasmonate signallingEcology
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