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City: Borstel 

  • Lipidomics MS Data processing, identification & quantification - Lipidomics Forum 2019

    10 November 2019

    Borstel, Germany

    Lipidomics MS Data processing, identification & quantification - Lipidomics Forum 2019 https://tess.elixir-europe.org/events/lipidomics-ms-data-processing-identification-quantification-lipidomics-forum-2019 Educators: Jacobo Miranda Ackermann, Nils Hoffmann (BioInfra.Prot / LIFS) Date: November 10th, 2019 Location: Forschungszentrum Borstel, Borstel, Germany (as part of Lipidomics Forum 2019) Contents: In this course, we will work through a typical use-case for bioinformatics processing lipidomics data following MS acquisition. The first part of the course will start with PeakStrainer and LipidXplorer for filtering, identification and quantification of lipid signals from shotgun lipidomics. The second part of the course will work through an example for targeted LC-MS lipidomics with LipidCreator and Skyline. The course will consist of a short theory and background overview of the employed programs complemented by the application of the tools to a provided data set. 10:00 - 10:45 Common Introduction 10:45 - 12:30 LipidXplorer Shotgun Lipidomics 12:30 - 13:30 Lunch break 13:30 - 16:30 LipidCreator & Skyline Targeted LC-MS lipidomics Learning goals: Participants will be able to understand and explain the shotgun MS and targeted LC-MS workflows for lipidomics. They will learn the fundamentals of the software tools used and how to choose parameters for them. They will learn to understand and interpret the results of each step of the pipeline. Prerequisites: Basic knowledge of lipidomics, analytical workflows in lipidomics and basic familiarity with web-based and desktop applications. Participants are required to bring their own laptop in order to work on the hands-on exercises. Keywords: Lipidomics, Shotgun, Targeted, LC-MS Tools: PeakStrainer and LipidXplorer, LipidCreator, Skyline 2019-11-10 09:00:00 UTC 2019-11-10 17:00:00 UTC de.NBI Borstel, Borstel, Germany Borstel Borstel Germany [] [] [] [] [] []
  • Statistical analysis & qualitative and quantitative comparison of lipidomics data - Lipidomics Forum 2019

    10 November 2019

    Borstel, Germany

    Statistical analysis & qualitative and quantitative comparison of lipidomics data - Lipidomics Forum 2019 https://tess.elixir-europe.org/events/statistical-analysis-qualitative-and-quantitative-comparison-of-lipidomics-data-lipidomics-forum-2019 Educators: Fadi al Machot, Nils Hoffmann (BioInfra.Prot / LIFS) Date: November 10th, 2019 Location: Forschungszentrum Borstel, Borstel, Germany (as part of Lipidomics Forum 2019) Contents: In this course, we will work through a typical use-case of downstream data processing of shotgun lipidomics data following MS acquisition with LipidXplorer. We will inspect, check and normalize the data as well as calculate absolute quantities using internal class-specific standards. We will then perform a qualitative comparison of the lipidomes using the LUX Score lipidome homology, before finally comparing and visualizing our quantitative lipidome results with ClovR. The course will consist of a short theory and background overview of the employed programs complemented by the application of the tools to provided data sets. 10:00 - 10:45 Common Introduction 10:45 - 12:30 R Scripting for LipidXplorer data quantification Quantitative lipidome comparison wiht ClovR 12:30 - 13:30 Lunch break 13:30 - 16:30 Qualitative lipidome with LUX Score Machine learning, clustering and classification for lipidomics data Learning goals: Participants will be able to understand and explain the shotgun lipidomics data analysis workflow. They will learn the fundamentals of the software tools used and how to choose parameters for them. They will learn to understand and interpret the results of each step of the pipeline. Prerequisites: Basic knowledge of lipidomics, analytical workflows in lipidomics and basic familiarity with web-based applications. Basic knowledge of R and RStudio is a plus to understand the quantitation workflow. Participants are required to bring their own laptop in order to work on the hands-on exercises. Keywords: Lipidomics, Statistical Analysis, Visualization Tools: R, RStudio, LUX Score, ClovR. 2019-11-10 09:00:00 UTC 2019-11-10 17:00:00 UTC de.NBI Borstel, Borstel, Germany Borstel Borstel Germany [] [] [] workshops_and_courses [] []
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