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  • Applied Metaproteomics Workshop 2020

    23 - 27 November 2020

    Magdeburg, Germany

    Applied Metaproteomics Workshop 2020 Educators: Dirk Benndorf, Stephan Fuchs, Robert Heyer, Kay Schallert (BiGi/MetaProtServ) Date: 23.11.2020 – 27.11.2020 Location: Magdeburg, Germany (Universitätsplatz 2, Building 28.205) Contents: Microbiome research is rapidly increasing. Meta-Omics data provide insights into microbial community influencing human health and biochemical cycle. Metaproteomics focusses on proteins representing the expressed genes and functions. In contrast to metagenomics or metatranscriptomics, the extraction and the analysis of proteins from human or environmental samples is challenging. The de.NBI course on Applied Metaproteomics trains the participants to apply a complete workflow starting from design of experiments via sample preparation to measurement and bioinformatics analysis of high-resolution MS data. Maximum 10 participants are strongly encouraged to analyze their own samples to start their own metaproteomics project. The workshop takes place at chair of bioprocess engineering at Otto-von-Guericke University Magdeburg. The team of microbial communities head by Dr. Dirk Benndorf is well experienced in metaproteomics. The samples will be measured on newly purchased timsTOF Pro MS. His group devellopes the MetaProteomAnalyzer service facilitating the analysis of of metaproteomic data. The provision of service is supported by the German Network for Bioinformatics Infrastructure - de.NBI. Monday 09:00-10:00 Arrival 10:00-11:00 Introduction to metaproteomics (lecture: Dirk Benndorf) 11:00-12:00 Design of experiments and sample preparation (lecture: Robert Heyer) 12:00-13:00 Lunch 13:00-18:00 Sample preparation I (protein extraction, protein quantification: Robert Heyer) during the afternoon short presentations of participant´s about their samples (each participant max. 10 min) Tuesday 08:00-11:00 Sample preparation II (FASP digest, SDS-PAGE: Robert Heyer) 11:00-13:00 Introduction to high resolution mass spectrometry (lecture: Dirk Benndorf) 13:00-14:00 Lunch 13:00-14:00 Sample preparation II (peptide extraction: Robert Heyer) 14:00-16:00 Introduction to the MPA 2.0 (lecture: Kay Schallert) non-stop: measurement of participant’s samples I Wednesday non-stop: measurement of participant’s samples I: Dirk Benndorf, Patrick Hellwig 09:00-11:00 MPA 2.0 exercise I (working with a small reference dataset, db searching, metaprotein concept: Kay Schallert, Robert Heyer) 11:00-13:00 MPA 2.0 exercise II (loading user db, BLAST search of unknown hits, quantitative comparison of datasets, visualization and data export: Kay Schallert, Robert Heyer) 13:00-14:00 Lunch 14:00-17:00 Prophane workflow (lecture and training: Stefan Fuchs, external speaker) Thursday 09:00-13:00 MPA 2.0 exercise III (analyzing own data: Kay Schallert, Robert Heyer) 13:00-14:00 Lunch 14:00-17:00 Training at MS (calibration, setup of LC-MS methods, solvent exchange, trouble shooting, Patrick Hellwig) Friday 08:00-10:00 MPA 2.0 exercise IV (visualization of data: Robert Heyer) 10:00-12:00 Individual discussion of results and preparation of presentations (Dirk Benndorf, Robert Heyer, Kay Schallert) 12:00-13:00 Short presentations of participant‘s results and conclusion 13:00 Lunch and departure Learning goals: To apply a complete workflow starting from design of experiments via sample preparation to measurement and bioinformatics analysis of high-resolution MS data. Prerequisites: Fee: 30 € Keywords: Microbiome, MPA, Metaproteomics, MetaProteomeAnalyzer, Tools: MetaproteomeAnalyzer 2020-11-23 09:00:00 UTC 2020-11-27 17:00:00 UTC de.NBI Magdeburg, Magdeburg, Germany Magdeburg Magdeburg Germany [] [] [] workshops_and_courses [] []
  • ELIXIR-UK All Hands 2020

    10 - 11 December 2020

    Norwich, United Kingdom

    ELIXIR-UK All Hands 2020 The annual ELIXIR-UK All Hands brings together people working at ELIXIR-UK consortium member institutions to - Share knowledge and experience from the previous year of ELIXIR activities - Work together to develop a strategy for the future of ELIXIR-UK - Updates from ELIXIR-UK and Technopolis on BioFAIR All members of ELIXIR-UK consortium member institutions working on ELIXIR-related projects are welcome to join the two-day meeting, which this year will be a virtual event. 2020-12-10 11:00:00 UTC 2020-12-11 17:00:00 UTC Earlham Institute Virtual Event, Norwich, United Kingdom Virtual Event Norwich United Kingdom NR4 7UZ Earlham Institute [] [] [] registration_of_interest []
  • Metagenomics

    31 May - 1 June 2021

    Leiden, Netherlands

    Elixir node event
    Metagenomics In this workshop, a general background to metagenomics is provided. The workflow of sample preparation, DNA isolation, platform selection, read mapping, annotation and metagenome assembly is covered. Attention is paid to taxonomic classification, quantification of microbial communities and species richness.… The post Metagenomics appeared first on Dutch Techcentre for Life Sciences. 2021-05-31 09:00:00 UTC 2021-06-01 00:00:00 UTC Leiden, Netherlands, Leiden, Netherlands Leiden, Netherlands Leiden Netherlands [] [] [] workshops_and_courses [] []
  • Long Reads Workshop 2021

    8 - 16 June 2021

    Norwich, United Kingdom

    Long Reads Workshop 2021 **This event has been postponed from 2020 due to the COVID-19 (coronavirus) pandemic. The speakers were confirmed for our 2020 event, and we are hopeful they will be able to attend in 2021.** This two-day workshop will provide a combination of presentation of use cases of long read RNAseq, technology developments from the Chief Scientific Officers of leading long read technology companies, sample processing hints and tips from the experts in sequencing facilities at Earlham Institute and Uppsala’s SciLifeLab. In addition we will provide some hands on examples of quantification, differential expression and resistance gene enrichment sequencing (e.g. RenSeq). Please email if you would like to be notified as soon as registrations and abstract submissions open. Who is this event for? Research staff and students who are current users of long read technology for the production of transcriptomic data and those analysing long read RNAseq data to produce genome annotations, differential expression analyses and those using RenSeq. We particularly encourage early career researchers and PhD students to submit abstracts for selection for oral presentation. This training forms part of our BBSRC National Capability in Advanced Training 2021-06-08 10:00:00 UTC 2021-06-16 17:00:00 UTC Earlham Institute Earlham Institute, Norwich, United Kingdom Earlham Institute Norwich United Kingdom NR4 7UZ Earlham Institute [] [] workshops_and_courses [] []
  • Norwich Single-Cell Symposium 2021

    15 - 16 September 2021

    Norwich, United Kingdom

    Norwich Single-Cell Symposium 2021 *This event has been postponed from 2020 due to the COVID-19 (coronavirus) pandemic. The speakers were confirmed for our 2020 event, and we are hopeful they will be able to attend in 2021.** Single-cell genomic technologies continue to develop at pace, generating new insights into cellular diversity within living systems. This event aims to bring together researchers applying single-cell technologies across a wide range of species. The symposium covers single-cell genomics technologies and their application in microbial, plant, animal and human health and disease. Offering a forum for researchers to discuss the latest developments in single-cell genomics, whilst networking with other researchers with the intention of catalysing future development and application of single-cell genomics across the UK. The event will feature talks from invited speakers and selected abstracts, and we are keen to capture as broad a range of single-cell applications as possible. Topics to be covered include: - Single-cell genomics in plant and microbial research - Single-cell genomics in health, disease and development - Single-cell informatics - Single-cell technology development Abstracts We are offering the opportunity to present your research at the symposium. This will either be as a 15-20 minute talk, or as a poster (you can select your preference during the registration). Submission deadline for abstracts is 1 August 2021. All abstracts must be submitted electronically as part of your registration. Submissions via email will not be accepted Abstract limits are 250 words (excluding title, authors and affiliations) Presenting author should be highlighted in bold Abstracts will be reviewed within 14 days of the submission deadline and you will be informed of the outcome shortly thereafter 2021-09-15 10:00:00 UTC 2021-09-16 17:00:00 UTC Earlham Institute Earlham institute, Norwich, United Kingdom Earlham institute Norwich United Kingdom NR4 7UZ Earlham Institute Molecular Machines & Industries (MMI) [] meetings_and_conferences [] []

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