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2 events found

Scientific topics: Sequencing 

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City: Gebze  or Seattle  or Oeiras  or Leiden  or Berlin 

  • 3DAROC16

    10 - 14 October 2016

    Oeiras, Portugal

    3DAROC16 https://tess.elixir-europe.org/events/3daroc16 3C-based methods, such as Hi-C, produce a huge amount of raw data as pairs of DNA reads that are in close spatial proximity in the cell nucleus. Overall, those interaction matrices have been used to study how the genome folds within the nucleus, which is one of the most fascinating problems in modern biology. The rigorous analysis of those paired-reads using computational tools has been essential to fully exploit the experimental technique, and to study how the genome is folded in the space. Currently, there is a clear expansion on the wealth of data on genome structure with the availability of many datasets of Hi-C experiments down to 1Kb resolution (see for example: http://hic.umassmed.edu/welcome/welcome.php ; http://promoter.bx.psu.edu/hi-c/view.php or http://www.aidenlab.org/data.html ). In this course, participants will learn to use TADbit, a software designed and developed to manage all dimensionalities of the Hi-C data: 1D - Map paired-end sequences to generate Hi-C interaction matrices 2D - Normalize matrices and identify constitutive domains (TADs, compartments) 3D - Generate populations of structures which satisfy the Hi-C interaction matrices 4D - Compare samples at different time points Participants can bring- specific biological questions and/or their own 3C-based data to analyze during the course. At the end of the course, participants will be familiar with the TADbit software and will be able to fully analyze Hi-C data. Note: Although the TADbit software is central in this course, alternative software will be discussed for each part of the analysis. 2016-10-10 09:00:00 UTC 2016-10-14 00:00:00 UTC Instituto Gulbenkian de Ciência Instituto Gulbenkian de Ciência, Oeiras, Portugal Instituto Gulbenkian de Ciência Oeiras Portugal 901 Sequencing [] [] [] workshops_and_courses [] Hi-Cdata
  • DNA Methylation Data Analysis Workshop

    2 - 5 November 2020

    Berlin, Germany

    DNA Methylation Data Analysis Workshop https://tess.elixir-europe.org/events/dna-methylation-data-analysis-workshop Advance your epigenetics research with NGS methods. In a nutshell - Learn how bisulfite sequencing works - Understand how bisulfite-treated reads are mapped to a reference genome - Perform basic analyses (call methylated regions, perform basic downstream analyses) - Use shell scripting to create reusable data pipelines - Visualize results (ready-to-publish) The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics. By the end of this workshop the participants will: - be familiar with the sequencing method of Illumina - understand how bisulfite sequencing works - be aware of the mapping problem of bisulfite-treated data - understand how bisulfite-treated reads are mapped to a reference genome - be familiar with common data formats and standards - know relevant tools for data processing - automate tasks with shell scripting to create reusable data pipelines - perform basic analyses (call methylated regions, perform basic downstream analyses) - plot and visualize results (ready-to-publish) - be able to reuse all analyses 2020-11-02 09:00:00 UTC 2020-11-05 17:00:00 UTC ecSeq Bioinformatics GmbH PC-COLLEGE Berlin, 78, Stresemannstraße, Berlin, Germany PC-COLLEGE Berlin, 78, Stresemannstraße Berlin Germany 10963 Sequencing Sequence analysis Bioinformatics [] ecSeq Bioinformatics GmbH Sternwartenstr. 29 D-04103 Leipzig Germany Email: events@ecSeq.com [] BiologistsBiologists, Genomicists, Computer ScientistsMolecular BiologistsPhD studentspost-docsPathologistsbioinformaticians 25 workshops_and_courses first_come_first_served EpigeneticsDNA MethylationBisulfite-Seq

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