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Event types: Workshops and courses 

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Organizer: de.NBI 

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City: Bogota  or Heidelberg  or Fribourg  or Basel  or Amsterdam UMC (location AMC)  or Norwich  or Utrecht  or Lund  or Kiel  or Gießen 

  • 6th de.NBI Genomics training course

    4 - 6 May 2020

    Gießen, Germany

    6th de.NBI Genomics training course https://tess.elixir-europe.org/events/6th-de-nbi-genomics-training-course Educators: Alexander Goesmann, Sven Griep, Oliver Rupp, Tobias Zimmermann, Oliver Schwengers, Andreas Hoek, Jochen Blom, Sebastian Jaenicke (BiGi) Date: 04.-06.05.2020 Location: Heinrich-Buff-Ring 58, Justus-Liebig-Universität, Gießen, Seminar Rm. 0024a Content: The first day will introduce software tools for the quality control of short read data. For conducting basic sequence read processing steps we will use our local Galaxy server. In the last session we will explain the basic approaches for genome assembly and run various genome assemblers: - Introduction to quality control - Automatic trimming of sequencing reads - The basics of genome assembly On the second day we will take a closer look at the genome assembly results. Afterwards, we provide a comprehensive introduction to microbial genome annotation. Available tools and algorithms for the different genome analysis and annotation steps will be explained and used within Galaxy. The GenDB genome annotation pipeline will be presented as well as a new stand-alone high-throughput genome analyses pipeline (ASA3P) that can be used to process thousands of genomes on a single day: - The basic principles of region and function annotation - Genome annotation tools and pipelines in Galaxy - Manual and distributed genome annotation using GenDB - Introduction to the ASA3P software The third day will give an introduction into comparative genomics with a focus on differential gene content analysis using the EDGAR platform. The use of the BLAST score ratio values (SRVs) to measure the similarity between coding sequences and to assess orthology will be introduced. All features of EDGAR will be introduced in detail: - Genomic subset calculations (core-genome, pan-genome, singleton genes) - Core-genome-based phylogenetic analyses - Sequence based phylogeny - ANI, AAI - Statistical analyses (core/pan/singleton development plots) - The public EDGAR database Agenda: Day 1: Quality control & genome assembly 13:00 – 13:15 Welcome & introduction (Alexander Goesmann) 13:15 – 13:30 Transfer of data sets 13:30 – 15:00 Read quality control & trimming incl. hands-on session (Sven Griep, Oliver Rupp) 15:00 – 15:30 Coffee Break 15:30 – 17:00 Introduction to genome assembly incl. hands-on session (Sven Griep, Oliver Rupp) Day 2: Microbial Genome Annotation 9:00 – 10:30 Introduction to microbial genome annotation (Alexander Goesmann) 10:30 – 11:00 Coffee Break 11:00 – 12:30 Genome annotation with Galaxy & GenDB hands-on session (Sven Griep, Oliver Rupp, Tobias Zimmermann) 12:30 – 13:30 Lunch 13:30 – 14:30 Introduction to ASA3P (Oliver Schwengers) 14:30 – 15:00 Coffee Break 15:00 – 17:00 ASA3P practical session (Oliver Schwengers, Andreas Hoek) 17:30 Optional: Tour to Gleiberg castle Day 3: Comparative Genomics 9:00 – 10:30 EDGAR (Jochen Blom) 10:30 – 11:00 Coffee Break 11:00 – 12:00 EDGAR hands-on (Jochen Blom, Sebastian Jaenicke) 12:00 – 12:30 Course evaluation & closing remarks (all hands) During the afternoon of the last day participants are invited to discuss open questions or individual projects or data sets with the course experts. Learning goals: On all three days the program includes extensive hands-on training to allow participants to get familiar with the presented software. Participants can bring their own datasets that fit the topics of the workshop. The data can be analyzed in the hands-on sessions. Prerequisites: None Keywords: Quality control, genome assembly, Microbial Genome Annotation, Comparative Genomics Tools: Galaxy, GenDB, ASA3P, EDGAR 2020-05-04 09:00:00 UTC 2020-05-06 17:00:00 UTC de.NBI Gießen, Gießen, Germany Gießen Gießen Gießen Germany [] [] [] workshops_and_courses [] []
  • Phylogenetic reconstruction course 2020

    20 May 2020

    Kiel, Germany

    Phylogenetic reconstruction course 2020 https://tess.elixir-europe.org/events/phylogenetic-reconstruction-course-2020 Educators: Tal Dagan (CAU) Date: 20.05.2019, 08:15 - 16:00 Location: Kiel Contents/Learning goals: The evolutionary history of genes or species is best studied and described by phylogenetic trees. The accumulating sequence data enable the reconstruction of phylogenetic trees for many diverse gene and species. A robust phylogeny is helpful in identifying phyletic groups as well as ancestral relations in the data and lateral gene transfer. Notwithstanding phylogenetic reconstruction is sensitive to various biases in the analysis that originate in alignment and phylogenetic reconstruction artifacts. Students in the workshop will learn to reconstruct multiple sequence alignment and phylogenetic trees of focal sequences. This includes a quantification of possible biases in the phylogenetic reconstruction and ways to counteract their influence on the results. In addition, the students will learn how to identify the root of phylogenetic trees and extract ancestral-descendent relations. The workshop is intended to address scientists from undergraduate to postdoc level and does not expect previous experience in phylogenetics. Basic knowledge on how to work with Linux will be advantageous. Prerequisites: None Keywords: Tree phylogeny, gene homology, rooting, robustness Tools: MAFFT, Guidance, PhyML, IQTree, MAD rooting, FigTree. 2020-05-20 09:00:00 UTC 2020-05-20 17:00:00 UTC de.NBI Kiel, Kiel, Germany Kiel Kiel Germany [] [] [] workshops_and_courses [] []

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