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Scientific topics: RNA-Seq 

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  • Training Course on Best practices for RNA-Seq data analysis

    27 - 29 September 2017

    Fisciano, Italy

    Training Course on Best practices for RNA-Seq data analysis https://tess.elixir-europe.org/events/training-course-on-best-practices-for-rna-seq-data-analysis Next-generation sequencing (NGS) of RNA libraries (RNA-Seq) has become increasingly common and it largely replaced microarray technology for gene expression profiling. The aim of this course is to get a deeper understanding of RNA-Seq experiments, providing a theoretical introduction to the data processing steps, together with practical sessions illustrating the use of the most popular data analysis tools. The classroom size is limited to 25 participants to optimize the learning and the interaction with the instructors. Starting from the raw sequenced data coming from different phenotypical samples (e.g disease vs healthy control samples), genes which are differentially expressed between the two conditions are determine. Some strategies are illustrated for detecting alternative splicing products, predicting novel isoforms and gene fusion events. Some methods for downstream analysis are described to give insight into how biological knowledge can be generated from RNA-Seq experiments. A lecture on single-cell RNA-Seq will provide an idea of how transcriptome data from individual cells is now emerging as a powerful tool, allowing the study of cell-to-cell gene expression heterogeneity in the same tissue. 2017-09-27 09:15:00 UTC 2017-09-29 16:30:00 UTC Anna Marabotti (University of Salerno and ELIXIR-IIB Training Team, Italy), Roberto Tagliaferri (University of Salerno, Italy), Alessandro Weisz (University of Salerno, Italy), Loredana Le Pera (ELIXIR-IIB Training Team and IIT, Italy), Allegra Via (ELIXIR-IIB Training Coordinator, CNR-IBPM, Italy) Campus di Fisciano, Università degli Studi di Salerno, Via Giovanni Paolo II, n. 132, Fisciano, Italy Campus di Fisciano, Università degli Studi di Salerno, Via Giovanni Paolo II, n. 132 Fisciano Provincia di Salerno Italy 84084 Transcriptomics RNA splicing Sequencing Bioinformatics RNA-Seq Gene expression Campus di Fisciano, University of Salerno, Italy elixir.ita.training@gmail.com, amarabotti@unisa.it ELIXIR Italy PhD students and young researchers in the life science and computational biology field who are planning to use RNA-seq data and are looking for the best practices to analyze these types of data 25 workshops_and_courses first_come_first_servedregistration_of_interest NGSRNA-SeqBioinformaticsData analysisHigh Throughput Sequencing AnalysisGene Expression
  • ELIXIR-EXCELERATE Train the Trainer

    27 - 29 September 2017

    Fisciano, Italy

    ELIXIR-EXCELERATE Train the Trainer https://tess.elixir-europe.org/events/elixir-excelerate-train-the-trainer ELIXIR-IIB, in cooperation with the ELIXIR-EXCELERATE Train-The-Trainer programme, will hold a Train the Trainer (TtT) event in connection with a course on “Best Practices for RNA-Seq analysis”. The course is made up of four sessions, covering: Learning principles and how they apply to training and teaching; Teaching techniques that can be used to enhance learner engagement and participation; Assessment and feedback in training and teaching; Lesson, course, and materials design. 2017-09-27 12:13:00 UTC 2017-09-29 12:13:00 UTC Allegra Via, Pedro Fernandes, Anna Marabotti, Roberto Tagliaferri, Loredana Le Pera Campus di Fisciano, Università di Salerno, Fisciano, Italy Campus di Fisciano, Università di Salerno Fisciano Provincia di Salerno Italy 84084 RNA-Seq RNA Campus di Fisciano, University of Salerno, Italy elixir.ita.training@gmail.com [] teachers / trainersAnyone who wants to become a teacher / trainer or a better one 15 workshops_and_courses registration_of_interest TtT, Train the Trainer, Educational psychology, cognitive science, teaching practices
  • Introduction to RNA-seq Data Analysis

    17 - 19 February 2020

    Edinburgh, United Kingdom

    Elixir node event
    Introduction to RNA-seq Data Analysis https://tess.elixir-europe.org/events/introduction-to-rna-seq-data-analysis-5989e86e-a408-481d-b3c7-5a1413b0e4a6 RNA sequencing (RNA-seq) is quickly becoming the method of choice for transcriptome profiling. Nevertheless, it is a non-trivial task to transform the vast amount of data obtained with high-throughput sequencers into useful information. Thus, RNA-seq data analysis is still a major bottleneck for most researchers in this field. The ability of correctly interpreting RNA-seq results, as well as knowledge on the intrinsic properties of these data, are essential to avoid incorrect experimental designs and the application of inappropriate analysis methodologies. The aim of this workshop is to familiarise researchers with RNA-seq data and to initiate them in the analysis by providing lectures and practicals on analysis methodologies. In the practicals Illumina-generated sequencing data and various widely used software programs will be used. 2020-02-17 09:00:00 UTC 2020-02-19 17:00:00 UTC Kings Buildings, The University of Edinburgh, Edinburgh, United Kingdom Kings Buildings, The University of Edinburgh Edinburgh United Kingdom EH25 9RG RNA-Seq Genomics Bioinformatics Edinburgh Genomics Edinburgh Genomics Training Team - edge-training@ed.ac.uk [] [] workshops_and_courses [] HDRUK

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