RNA-seq Analysis for Beginners
Start: Monday, 04 March 2019 @ 09:30
End: Monday, 04 March 2019 @ 17:00
Venue: Pam Liversidge Building, Design Studio 1, D06
Country: United Kingdom
Postcode: S3 7RDDescription:
High-throughput RNA-sequencing is now the standard technique for quantifying transcript abundance in a biological sample of interest. In this course we will describe the processes that take place once you submit a library for RNA sequencing, and what data you should expect to receive from the Bioinformatics Core. We will describe the steps involved to go from sequencing library to a list of genes that show statistically significant differences between your biological conditions of interest. Practical sessions will use the user-friendly Galaxy interface (https://usegalaxy.org/) to demonstrate tasks such as alignment, quality control and assessing differential expression. We will also showcase some web sites you can use for enrichment and pathways analysis.
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