Organizer: ecSeq Bioinformatics GmbH

Start: Monday, 02 November 2020 @ 09:00

End: Thursday, 05 November 2020 @ 17:00

Timezone: Berlin

Contact: ecSeq Bioinformatics GmbH Sternwartenstr. 29 D-04103 Leipzig Germany Email: events@ecSeq.com

Venue: PC-COLLEGE Berlin, 78, Stresemannstraße

City: Berlin

Country: Germany

Postcode: 10963

Scientific topic: Sequencing, Sequence analysis, Bioinformatics

Target audience:
  • Biologists
  • Biologists, Genomicists, Computer Scientists
  • Molecular Biologists
  • PhD students
  • post-docs
  • Pathologists
  • bioinformaticians
Description:

Advance your epigenetics research with NGS methods.

In a nutshell
- Learn how bisulfite sequencing works
- Understand how bisulfite-treated reads are mapped to a reference genome
- Perform basic analyses (call methylated regions, perform basic downstream analyses)
- Use shell scripting to create reusable data pipelines
- Visualize results (ready-to-publish)

The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics.

By the end of this workshop the participants will:
- be familiar with the sequencing method of Illumina
- understand how bisulfite sequencing works
- be aware of the mapping problem of bisulfite-treated data
- understand how bisulfite-treated reads are mapped to a reference genome
- be familiar with common data formats and standards
- know relevant tools for data processing
- automate tasks with shell scripting to create reusable data pipelines
- perform basic analyses (call methylated regions, perform basic downstream analyses)
- plot and visualize results (ready-to-publish)
- be able to reuse all analyses

Event type:
  • Workshops and courses

Capacity: 25

Eligibility:
  • First come first served

Keywords: Epigenetics, DNA Methylation, Bisulfite-Seq

DNA Methylation Data Analysis Workshop https://tess.elixir-europe.org/events/dna-methylation-data-analysis-workshop Advance your epigenetics research with NGS methods. In a nutshell - Learn how bisulfite sequencing works - Understand how bisulfite-treated reads are mapped to a reference genome - Perform basic analyses (call methylated regions, perform basic downstream analyses) - Use shell scripting to create reusable data pipelines - Visualize results (ready-to-publish) The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics. By the end of this workshop the participants will: - be familiar with the sequencing method of Illumina - understand how bisulfite sequencing works - be aware of the mapping problem of bisulfite-treated data - understand how bisulfite-treated reads are mapped to a reference genome - be familiar with common data formats and standards - know relevant tools for data processing - automate tasks with shell scripting to create reusable data pipelines - perform basic analyses (call methylated regions, perform basic downstream analyses) - plot and visualize results (ready-to-publish) - be able to reuse all analyses 2020-11-02 09:00:00 UTC 2020-11-05 17:00:00 UTC ecSeq Bioinformatics GmbH PC-COLLEGE Berlin, 78, Stresemannstraße, Berlin, Germany PC-COLLEGE Berlin, 78, Stresemannstraße Berlin Germany 10963 Sequencing Sequence analysis Bioinformatics [] ecSeq Bioinformatics GmbH Sternwartenstr. 29 D-04103 Leipzig Germany Email: events@ecSeq.com [] BiologistsBiologists, Genomicists, Computer ScientistsMolecular BiologistsPhD studentspost-docsPathologistsbioinformaticians 25 workshops_and_courses first_come_first_served EpigeneticsDNA MethylationBisulfite-Seq